Treadmill training attenuates pyroptosis in rats with cerebral ischemia/reperfusion injury | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 8، دوره 25، شماره 10، دی 2022، صفحه 1215-1221 اصل مقاله (1.14 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2022.64668.14231 | ||
| نویسندگان | ||
| Fang Luo؛ Mingjin Zhu؛ Kunkun Lv؛ Di Sun؛ Guifen Yang؛ Guoyuan Pan* | ||
| Tongde Hospital of Zhejiang Province, No. 234, Gucui Road, Hangzhou, Zhejiang, China | ||
| چکیده | ||
| Objective(s): Few studies have investigated the mechanism by which exercise training promotes neural repair during rehabilitation after stroke. In this study, we evaluated the neuroprotective effects of exercise training and pyroptosis-associated factors in the penumbra and elucidated the possible mechanisms. Materials and Methods: Neurological deficits, body weight, and the infarct size were evaluated, and haematoxylin-eosin (HE) staining was performed. Western blotting and immunofluorescence staining were used to assess NOD-like receptor family pyrin domain-containing 3 (NLRP3) and caspase-1 levels. Interleukin-1β (IL-1β) and interleukin-18 (IL-18) levels were assessed by enzyme-linked immunosorbent assay (ELISA). B-cell lymphoma 2 (bcl-2) and bax protein levels were measured by Western blotting, and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) staining was used to evaluate apoptotic cells. Results: Exercise training decreased neurological deficits and the infarct size in MCAO rats Moreover, NLRP3 inflammasome-associated protein levels in the peri-infarct cortex were decreased by exercise training. Exercise training decreased the serum concentrations of IL‑1β and IL‑18, upregulated bcl-2, downregulated bax, and reduced the TUNEL index. Conclusion: Exercise training suppresses NLRP3 inflammasome activity and inhibits pyroptosis to protect against cerebral ischaemic injury. Exercise training can also suppress apoptosis, which may be the target of exercise-induced neuroprotection, thereby reducing brain injury. | ||
| کلیدواژهها | ||
| Apoptosis؛ Cerebral infarction؛ Exercise؛ NLR family؛ Protein؛ Pyroptosis؛ Pyrin domain containing 3 | ||
| مراجع | ||
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