سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Aslani, Mohammad Reza, Jafari, Zahra, Rahbarghazi, Reza, Rezaie, jafar, Delkhosh, Aref, Ahmadi, Mahdi. (1401). Effects of crocin on T-bet/GATA-3 ratio, and miR-146a and miR-106a expression levels in lung tissue of ovalbumin-sensitized mice. , 25(10), 1267-1274. doi: 10.22038/ijbms.2022.65622.14433
Mohammad Reza Aslani; Zahra Jafari; Reza Rahbarghazi; jafar Rezaie; Aref Delkhosh; Mahdi Ahmadi. "Effects of crocin on T-bet/GATA-3 ratio, and miR-146a and miR-106a expression levels in lung tissue of ovalbumin-sensitized mice". , 25, 10, 1401, 1267-1274. doi: 10.22038/ijbms.2022.65622.14433
Aslani, Mohammad Reza, Jafari, Zahra, Rahbarghazi, Reza, Rezaie, jafar, Delkhosh, Aref, Ahmadi, Mahdi. (1401). 'Effects of crocin on T-bet/GATA-3 ratio, and miR-146a and miR-106a expression levels in lung tissue of ovalbumin-sensitized mice', , 25(10), pp. 1267-1274. doi: 10.22038/ijbms.2022.65622.14433
Aslani, Mohammad Reza, Jafari, Zahra, Rahbarghazi, Reza, Rezaie, jafar, Delkhosh, Aref, Ahmadi, Mahdi. Effects of crocin on T-bet/GATA-3 ratio, and miR-146a and miR-106a expression levels in lung tissue of ovalbumin-sensitized mice. , 1401; 25(10): 1267-1274. doi: 10.22038/ijbms.2022.65622.14433

Effects of crocin on T-bet/GATA-3 ratio, and miR-146a and miR-106a expression levels in lung tissue of ovalbumin-sensitized mice

Iranian Journal of Basic Medical Sciences
مقاله 15، دوره 25، شماره 10، دی 2022، صفحه 1267-1274    اصل مقاله (858.3 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.65622.14433
نویسندگان
Mohammad Reza Aslani1، 2؛ Zahra Jafari3، 4؛ Reza Rahbarghazi5، 6؛ jafar Rezaie7؛ Aref Delkhosh6؛ Mahdi Ahmadi* 6
1Lung Diseases Research Center, Ardabil University of Medical Sciences, Ardabil, Iran
2Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
4Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
5Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
6Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
7Solid Tumor Research Center, Research Institute for Cellular and Molecular Medicine, Urmia University of Medical Sciences, Urmia, Iran
چکیده
Objective(s): Although various studies have revealed the beneficial effects of crocin (derived from saffron), such as anti-inflammatory, anti-cancer, antioxidant, and immune modulator, however, its exact mechanism is unknown. The present study aimed to investigate the effect of crocin on the expression ratio of T-bet/GATA-3 as an indicator of altered immune responses in the lung tissue of ovalbumin (OVA)-sensitized mice. In addition, the effect of crocin on the expression level of miR-146a and miR-106a in the lung tissue OVA-sensitized mice was investigated.
Materials and Methods: Mice were randomly divided into five groups (n=6): Control; OVA, OVA + Crocin 25, OVA + Cro 50, and OVA + Cro100 groups. Crocin was administrated intraperitoneally at doses of 25, 50, and 100 mg/kg for five consecutive days. One day after asthma induction, animals were euthanized, and lungs were sampled for pathological and gene expression analysis.
Results: OVA-sensitization led to increased inflammation and histopathological changes in the lung tissue of mice. In addition, GATA-3 expression increased (P<0.001) and T-bet expression decreased (P<0.001) in OVA-sensitized groups. The T-bet/GATA3 ratio was also reduced markedly in asthma groups (P<0.001). Furthermore, increased expression of miR-146a and miR-106a levels was evident in the lung tissue of OVA-sensitized mice (P<0.001 for both). Intervention with high concentrations of crocin (50 and 100 mg/kg) significantly reduced airway inflammation, GATA-3 expression, miR-146a expression, and miR-106a expression and corrected the T-bet/GATA-3 ratio (P<0.05 to P<0.001).
Conclusion: Treatment with crocin led to a decrease in the severity of lung inflammation in OVA-sensitized mice, which is probably through the reduction of the T-bet/GATA-3 ratio, and mir-146a and mir-106a expression level.
کلیدواژه‌ها
Asthma؛ GATA-3؛ microRNA-106a؛ microRNA-146a؛ T-box transcription factor
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