سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Zhao, Chunming, Liu, Jialin, Xu, Yong, Guo, Jiamei, Wang, Liping, Chen, Linfen, Xu, Lina, Dong, Guokai, Zheng, Wei, Li, Zhouru, Cai, Hongxing, Li, Shanshan. (1401). MiR-574-5p promotes cell proliferation by negatively regulating small C-terminal domain phosphatase 1 in esophageal squamous cell carcinoma. , 25(10), 1243-1250. doi: 10.22038/ijbms.2022.65886.14492
Chunming Zhao; Jialin Liu; Yong Xu; Jiamei Guo; Liping Wang; Linfen Chen; Lina Xu; Guokai Dong; Wei Zheng; Zhouru Li; Hongxing Cai; Shanshan Li. "MiR-574-5p promotes cell proliferation by negatively regulating small C-terminal domain phosphatase 1 in esophageal squamous cell carcinoma". , 25, 10, 1401, 1243-1250. doi: 10.22038/ijbms.2022.65886.14492
Zhao, Chunming, Liu, Jialin, Xu, Yong, Guo, Jiamei, Wang, Liping, Chen, Linfen, Xu, Lina, Dong, Guokai, Zheng, Wei, Li, Zhouru, Cai, Hongxing, Li, Shanshan. (1401). 'MiR-574-5p promotes cell proliferation by negatively regulating small C-terminal domain phosphatase 1 in esophageal squamous cell carcinoma', , 25(10), pp. 1243-1250. doi: 10.22038/ijbms.2022.65886.14492
Zhao, Chunming, Liu, Jialin, Xu, Yong, Guo, Jiamei, Wang, Liping, Chen, Linfen, Xu, Lina, Dong, Guokai, Zheng, Wei, Li, Zhouru, Cai, Hongxing, Li, Shanshan. MiR-574-5p promotes cell proliferation by negatively regulating small C-terminal domain phosphatase 1 in esophageal squamous cell carcinoma. , 1401; 25(10): 1243-1250. doi: 10.22038/ijbms.2022.65886.14492

MiR-574-5p promotes cell proliferation by negatively regulating small C-terminal domain phosphatase 1 in esophageal squamous cell carcinoma

Iranian Journal of Basic Medical Sciences
مقاله 12، دوره 25، شماره 10، دی 2022، صفحه 1243-1250    اصل مقاله (1.29 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.65886.14492
نویسندگان
Chunming Zhao1، 2؛ Jialin Liu3، 2؛ Yong Xu3، 2؛ Jiamei Guo3، 2؛ Liping Wang4؛ Linfen Chen3، 2؛ Lina Xu5؛ Guokai Dong3، 2؛ Wei Zheng6؛ Zhouru Li3، 2؛ Hongxing Cai* 3، 2؛ Shanshan Li* 3، 2
1Department of Human Anatomy, Xuzhou Medical University, Xuzhou, Jiangsu, China
2Jiangsu Medical Engineering Research Center of Gene Detection, Xuzhou Medical University, Xuzhou, Jiangsu, China
3Department of Forensic Medicine, Xuzhou Medical University, Xuzhou, Jiangsu, China
4Department of Basic Pathology, Pathology College, Qiqihar Medical University, Qiqihar, Heilongjiang, China
5NGS center, Hangzhou D.A. Medical Laboratory Co., Ltd., Hangzhou, Zhejiang, China
6Department of Orthopedics, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, China
چکیده
Objective(s): Esophageal cancer is one of the most common cancers with high incidence and mortality rates, especially in China. MicroRNA (miRNA) can be used as a prognostic marker for various human cancers. This study aims to detect suitable miRNA markers for esophageal squamous cell carcinoma (ESCC). 
Materials and Methods: Our previous gene expression data of ESCC cells and the data from GSE43732 and GSE112840 were analyzed. The expression of miR-574-5p in ESCC patients and controls was analyzed by real-time quantitative PCR. The effect of miR-574-5p on proliferation was detected by real-time cell analysis (RTCA) and EdU proliferation assay after cell transfections. The target gene small C-terminal domain phosphatase 1 (CTDSP1) of miR-574-5p was validated by luciferase reporter assay and western blotting.
Results: In the current study, the bioinformatics analysis found miR-574-5p up-regulated in ESCC. The qPCR assay of 26 ESCC and 13 adjacent/ normal tissues confirmed these results. We further demonstrated that miR-574-5p overexpression promoted cell proliferation. Then the dual-luciferase reporter assay and the rescue experiment suggested that CTDSP1 was a direct target of miR-574-5p.
Conclusion: MiR-574-5p played an oncological role in ESCC by interacting and negatively regulating CTDSP1. These results provided a deeper understanding of the effect of miR-574-5p on ESCC.
کلیدواژه‌ها
Cell proliferation؛ CTDSP1 protein؛ Esophageal squamous cell carcinoma؛ MiR-574-5p؛ Oncogene Protein v-akt
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