Nigella sativa and its main constituent, thymoquinone protect against glycerol-induced acute kidney injury in rats | ||
| Avicenna Journal of Phytomedicine | ||
| دوره 12، شماره 6، بهمن و اسفند 2022، صفحه 638-648 اصل مقاله (1.18 M) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2022.20921 | ||
| نویسندگان | ||
| Elham Naderi1؛ Abolfazl Khajavi Rad* 2؛ Somayeh Nazari3؛ Majid Khazaei4؛ Samira Shahraki5؛ Sara Hosseinian* 6 | ||
| 1Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. | ||
| 2Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3Medicinal & Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 4Department of Physiology, Mashhad University of Medical Sciences, Mashhad, Iran. Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 5Department of Physiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran | ||
| 6Department of physiology, School of medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| چکیده | ||
| Objective: Rhabdomyolysis is a life-threatening disease caused by releasing myoglobin from injured myocytes, which results in acute kidney injury. In this study, the effect of aqueous-alcoholic extract of Nigella sativa (NS) and thymoquinone (TQ) on rhabdomyolysis-induced kidney damage in rats was investigated. Materials and Methods: There were five groups of rats (n=8): Control, rhabdomyolysis, rhabdomyolysis treated with NS aqueous-alcoholic extract (200 and 400 mg/kg) and TQ (15 mg/kg). Treatments were given for 7 days (two days before and four days after glycerol injection). Glycerol was injected intramuscularly on the third day of the experiment for induction of rhabdomyolysis. Renal function parameters on the first, fourth, and seventh days of the experiment and renal oxidative stress and histological changes at the end of this study were assessed. Results: Glycerol injection caused a significant increase in serum level of urea, creatinine, creatine phosphokinase, urine output and tissue MDA compared to the control animals (p<0.05-0.001). Administration of NS extract and TQ significantly decreased serum urea and creatinine on days 4 and 7, creatine phosphokinase on day 4, and urine output on day 7 compared to the rhabdomyolysis group (p<0.05-0.001). Compared to the rhabdomyolysis group, treatment with NS extract and TQ improved kidney histological abnormalities (p<0.01-0.001). The catalase enzyme activity in the group treated with NS 400 mg/kg and thiol content in the NS 400 mg/kg and TQ groups were significantly higher than those of the rhabdomyolysis group (p<0.05-0.01). Conclusion: NS extract and to some extent TQ protect the kidney from rhabdomyolysis-induced injury. | ||
| کلیدواژهها | ||
| Nigella sativa؛ Thymoquinone؛ Rhabdomyolysis | ||
| مراجع | ||
|
Aebi H. 1984. Catalase in vitro. Methods Enzymol, 105: 121-126. Aydogdu N, Atmaca G, Yalcin O, Taskiran R, Tastekin E, Kaymak K. 2006. Protective effect of L-carnitine on myoglobinuric acute renal failure in rats. Clin Exp Pharmacol Physiol, 33: 119-124. Baliga R, Ueda N, Walker PD, Shhah SV. 1999. Oxidant mechanisms in toxic acute renal failure. Drug Metab Rev, 31: 971-997. Benhelima A, Kaid-Omar Z, Hemida H, Benmahdi T, Addou A. 2016. Nephroprotective and diuretic effect of Nigella sativa L seeds oil on lithiasic wistar rats. Afr J Tradit Complement Altern Med, 13: 204-214. Chander V, Singh D, Chopra K. 2003. Catechin, a natural antioxidant protects against rhabdomyolysis-induced myoglobinuric acute renal failure. Pharmacol Res, 48: 503-509. Coco TJ, Klasner AE. 2004. Drug-induced rhabdomyolysis. Curr Opin Pediatr, 16: 206-210. Ghorbanli M, Babaie A, Babakhanloo P, Mirza M. 1999. Effects of water stress on Nigella sativa L. growth and development, quantity and quality of essential oil, and amount of seed oil. Iran J Agric Sci, 30: 585-593. Gburek J, Birn H, Verroust PJ, Goj B, Jacobsen C, Moestrup SK, Willnow TE, Christensen EI. 2003. Renal uptake of myoglobin is mediated by the endocytic receptors megalin and cubilin. Am J Physiol Renal Physiol, 285: F451-F458. Homsi E, Dias EPO, Garcia WE, Gontijo JA, Figueiredo JF. 1996. Effects of nifedipine and platelet activating factor antagonist (BN 52021) in glycerol-induced acute renal failure in rats. Ren Fail, 18: 883-892. Hosseini SM, Taghiabadi E, Abnous K, Timcheh Hariri A, Pourbakhsh H, Hosseinzadeh H. 2017. Protective effect of thymoquinone, the active constituent of Nigella sativa fixed oil, against ethanol toxicity in rats. Iran J Basic Med Sci, 20: 927-939. Hosseinian S, Ebrahimzadeh Bideskan A, Shafei MN, Sadeghnia HR, Soukhtanloo M, Shahraki S, Smadi Noshahr Z, Khajavi Rad A. 2018. Nigella sativa extract is a potent therapeutic agent for renal inflammation, apoptosis, and oxidative stress in a rat model of unilateral ureteral obstruction. Phytother Res, 32: 2290- 2298. Hosseinian S, Khajavi Rad A, Ebrahimzadeh Bideskan A, Soukhtanloo M, Sadeghnia Naderi et al. AJP, Vol. 12, No. 6, Nov-Dec 2022 648 HR, Shafei MN, Motejadded F, Mohebbati R, Shahraki S, Beheshti F. 2017. Thymoquinone ameliorates renal damage in unilateral ureteral obstruction in rats. Pharmacol Rep, 69: 648-657. J Sedlak, R H Lindsay. 1968, Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem, 25: 192-205. Khan F. 2009. Rhabdomyolysis: a review of the literature. Neth J Med, 67: 272-283. Korrapati MC, Shaner BE, Schnellmann RG. 2012. Recovery from glycerol-induced acute kidney injury is accelerated by suramin. J Pharmacol Exp Ther, 341: 126- 136. Madesh M, Balasubramanian K. 1998. Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J Biochem Biophys, 35: 184-188. Mousleh R, Al Laham S, Al-Manadili A. 2018. The preventive role of pioglitazone in glycerol-induced acute kidney injury in rats during two different treatment periods. Iran J Med Sci, 43: 184-194. Paller MS. 1988. Hemoglobin-and myoglobininduced acute renal failure in rats: role of iron in nephrotoxicity. Am J Physiol Renal Physiol, 255: F539-F544. Panizo N, Rubio-Navarro A, Amaro-Villalobos JM, Egido J, Moreno JA. 2015. Molecular mechanisms and novel therapeutic approaches to rhabdomyolysis-induced acute kidney injury. Kidney Blood Press Res, 40: 520-532. Parekh R, Care DA, Tainter CR. 2012. Rhabdomyolysis: advances in diagnosis and treatment. Emerg Med Pract, 14: 1-15. Petejova N, Martinek A. 2014. Acute kidney injury due to rhabdomyolysis and renal replacement therapy: a critical review. Crit Care, 18: 224. Ragheb A, Attia A, Eldin WS, Elbarbry F, Gazarin S, Shoker A. 2009. The protective effect of thymoquinone, an anti-oxidant and anti-inflammatory agent, against renal injury: a review. Saudi J Kidney Dis Transpl, 20: 741-752. Rao Konda V, Arunachalam R, Eerike M, Rao R, Radhakrishnan AK, Raghuraman LP, Devi S. 2016. Nephroprotective effect of ethanolic extract of Azima tetracantha root in glycerol induced acute renal failure in Wistar albino rats. J Trad Complement Med, 6: 347-354. Singh AP, Muthuraman A, Jaggi AS, Singh N, Grover K, Dhawan R. 2012. Animal models of acute renal failure. Pharmacol Rep, 64: 31-44. Uchiyama M, Mihara M. 1978. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem, 86: 271-278. Wang YD, Zhang L, Cai GY, Zhang X, Lv Y, Hong Q, Shi S, Yin Z, Liu X, Chen X. 2011. Fasudil ameliorates rhabdomyolysis-induced acute kidney injury via inhibition of apoptosis. Ren Fail, 33: 811-818. Yavuz OY, Aydoğdu N, Taştekin E, Süt N. 2018. The effects of baicalin on myoglobinuric acute renal failure in rats. Balkan Med J, 35: 68-76 Yildiz F, Coban S, Terzi A, Savas M, Bitiren M, Celik H, Aksoy N. 2010. Protective effects of Nigella sativa against ischemiareperfusion injury of kidneys. Ren Fail, 32: 126-131. Zager RA. 1996. Rhabdomyolysis and myohemoglobinuric acute renal failure. Kidney Int, 49: 314-326 | ||
|
آمار تعداد مشاهده مقاله: 24,282 تعداد دریافت فایل اصل مقاله: 9,645 |
||