سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Basardeh, Eilnaz, Piri-Gavgani, Somayeh, Soltanmohammadi, Behnoush, Ghanei, Mostafa, Omrani, Mir Davood, Soezi, Mahdieh, Shokrgozar, Mohammad Ali, Azizi, Maasoumeh, Fateh, Abolfazl, Vaziri, Farzam, Siadat, Seyed Davar, Sharifzadeh, Zahra, Rahimi-Jamnani, Fatemeh. (1401). Anti-Acinetobacter baumannii single-chain variable fragments show direct bactericidal activity. , 25(9), 1141-1149. doi: 10.22038/ijbms.2022.64062.14106
Eilnaz Basardeh; Somayeh Piri-Gavgani; Behnoush Soltanmohammadi; Mostafa Ghanei; Mir Davood Omrani; Mahdieh Soezi; Mohammad Ali Shokrgozar; Maasoumeh Azizi; Abolfazl Fateh; Farzam Vaziri; Seyed Davar Siadat; Zahra Sharifzadeh; Fatemeh Rahimi-Jamnani. "Anti-Acinetobacter baumannii single-chain variable fragments show direct bactericidal activity". , 25, 9, 1401, 1141-1149. doi: 10.22038/ijbms.2022.64062.14106
Basardeh, Eilnaz, Piri-Gavgani, Somayeh, Soltanmohammadi, Behnoush, Ghanei, Mostafa, Omrani, Mir Davood, Soezi, Mahdieh, Shokrgozar, Mohammad Ali, Azizi, Maasoumeh, Fateh, Abolfazl, Vaziri, Farzam, Siadat, Seyed Davar, Sharifzadeh, Zahra, Rahimi-Jamnani, Fatemeh. (1401). 'Anti-Acinetobacter baumannii single-chain variable fragments show direct bactericidal activity', , 25(9), pp. 1141-1149. doi: 10.22038/ijbms.2022.64062.14106
Basardeh, Eilnaz, Piri-Gavgani, Somayeh, Soltanmohammadi, Behnoush, Ghanei, Mostafa, Omrani, Mir Davood, Soezi, Mahdieh, Shokrgozar, Mohammad Ali, Azizi, Maasoumeh, Fateh, Abolfazl, Vaziri, Farzam, Siadat, Seyed Davar, Sharifzadeh, Zahra, Rahimi-Jamnani, Fatemeh. Anti-Acinetobacter baumannii single-chain variable fragments show direct bactericidal activity. , 1401; 25(9): 1141-1149. doi: 10.22038/ijbms.2022.64062.14106

Anti-Acinetobacter baumannii single-chain variable fragments show direct bactericidal activity

Iranian Journal of Basic Medical Sciences
مقاله 14، دوره 25، شماره 9، آذر 2022، صفحه 1141-1149    اصل مقاله (775.57 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.64062.14106
نویسندگان
Eilnaz Basardeh1، 2؛ Somayeh Piri-Gavgani1، 2؛ Behnoush Soltanmohammadi1، 2؛ Mostafa Ghanei3؛ Mir Davood Omrani4؛ Mahdieh Soezi1، 2؛ Mohammad Ali Shokrgozar5؛ Maasoumeh Azizi6؛ Abolfazl Fateh1، 2؛ Farzam Vaziri1، 2؛ Seyed Davar Siadat1، 2؛ Zahra Sharifzadeh7؛ Fatemeh Rahimi-Jamnani* 1، 2
1Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
2Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran
3Chemical Injuries Research Center, Systems Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
4Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
6Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
7Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
چکیده
Objective(s): The high resistance rate of Acinetobacter baumannii and the limited number of available antibiotics have prompted a worldwide effort to develop effective antimicrobial agents. Accordingly, identifying single-chain variable fragment antibodies (scFvs), capable of exerting direct antibacterial activity in an immune system-independent manner, may be making immunocompromised patients more susceptible to A. baumannii infections.
Materials and Methods: To isolate bactericidal scFvs targeting A. baumannii, we panned a large human scFv phage display library against whole-cell extensively drug-resistant (XDR) A. baumannii strains grown as biofilm or cultured with human blood or human peripheral blood mononuclear cells plus plasma. The binding of scFv-phages to A. baumannii was assessed by the dot-blot assay. Soluble scFvs, derived from the selected phages, were assessed based on their ability to bind and inhibit the growth of A. baumannii. 
Results: Five phage clones showed the highest reactivity toward A. baumannii. Among five soluble scFvs, derived from positive phage clones, two scFvs, EB211 and EB279, had high expression yields and displayed strong binding to A. baumannii compared with the controls. Moreover, XDR A. baumannii strains treated with positively-charged scFvs, including EB211, EB279, or a cocktail of EB211 and EB279 (200 µg/ml), displayed lower viability (approximately 50%, 78%, and 40% viability, respectively) compared with PBS-treated bacteria.
Conclusion: These results suggest that combining last-resort antibiotics with bactericidal scFvs could provide promising outcomes in immunocompromised individuals with A. baumannii infections.
کلیدواژه‌ها
Acinetobacter baumannii؛ Antibacterial agents؛ Colistin؛ Monoclonal antibody؛ Phage display library؛ Single-chain variable-fragment
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