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Bahreyni-Toossi, Mohammad-Taghi, Dayyani, Mahdieh, Mahmoudi, Mahmoud, Azimian, Hosein. (1401). Flow cytometric assessment of DNA double-strand break and repair kinetics in prediction of intrinsic radiosensitivity. , 25(9), 1104-1109. doi: 10.22038/ijbms.2022.65178.14350
Mohammad-Taghi Bahreyni-Toossi; Mahdieh Dayyani; Mahmoud Mahmoudi; Hosein Azimian. "Flow cytometric assessment of DNA double-strand break and repair kinetics in prediction of intrinsic radiosensitivity". , 25, 9, 1401, 1104-1109. doi: 10.22038/ijbms.2022.65178.14350
Bahreyni-Toossi, Mohammad-Taghi, Dayyani, Mahdieh, Mahmoudi, Mahmoud, Azimian, Hosein. (1401). 'Flow cytometric assessment of DNA double-strand break and repair kinetics in prediction of intrinsic radiosensitivity', , 25(9), pp. 1104-1109. doi: 10.22038/ijbms.2022.65178.14350
Bahreyni-Toossi, Mohammad-Taghi, Dayyani, Mahdieh, Mahmoudi, Mahmoud, Azimian, Hosein. Flow cytometric assessment of DNA double-strand break and repair kinetics in prediction of intrinsic radiosensitivity. , 1401; 25(9): 1104-1109. doi: 10.22038/ijbms.2022.65178.14350

Flow cytometric assessment of DNA double-strand break and repair kinetics in prediction of intrinsic radiosensitivity

Iranian Journal of Basic Medical Sciences
مقاله 9، دوره 25، شماره 9، آذر 2022، صفحه 1104-1109    اصل مقاله (856.02 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.65178.14350
نویسندگان
Mohammad-Taghi Bahreyni-Toossi1؛ Mahdieh Dayyani2؛ Mahmoud Mahmoudi3؛ Hosein Azimian* 1
1Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2Reza Radiotherapy Oncology Center, Mashhad, Iran
3Immunology Research Center, Bu-Ali Research Institute, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): To enhance the efficiency of radiotherapy (RT), implementation of individual-based treatment is essential. In this way, determining individual intrinsic radiosensitivity (IRS) can be useful to achieve minimal adverse effects of RT. The present study aimed to identify IRS of breast cancer (BC) patients through determination of radiation-induced DNA double-strand breaks (DSBs), repair kinetics, and acute normal tissue complications induced by RT.
Materials and Methods: DSBs induction and its repair kinetics in 50 BC patients’ lymphocytes were analyzed by flow cytometric analysis of H2AX Ser-139 phosphorylation at 30 min, 3 and 24 hr after in vitro irradiation. In vivo skin dosimetry was done by GAFChromic films and acute skin toxicity was scored by radiation oncologists according to the criteria of Radiation Therapy and Oncology Group (RTOG) in all patients with similar prescribed treatment.
Results: The average surface dose for patients ranged from 0.92 to 1.9 Gy and correlation analysis showed no significant relationship with weekly acute skin reactions. Formation of γH2AX after 30 min, slope of dose-response curve and repair kinetics of DSBs after 3 and 24 hr (intrinsic radiosensitivity) were significantly correlated with the RTOG scores following irradiation (clinical radiosensitivity) (r=0.48 and P-value<0.0001, r=0.72 and P-value<0.0001, r=0.48 and P-value<0.001, and finally r=0.53 and P-value<0.001, respectively; (using Pearson’s correlation test). 
Conclusion: Flow cytometric analysis of DNA DSBs by γH2AX measurement has the potential to be developed into a clinical predictor for identifying the overreactor patients prior to RT. Our result suggests that the slope-related quantity based on the linear pattern of the dose-response curve has the merit to predict overreactor patients with a sensitivity of 89% and a specificity of 94%.
کلیدواژه‌ها
Breast Cancer؛ DNA Damage؛ Double-Strand Break؛ Radiosensitivity؛ Radiotherapy
سایر فایل های مرتبط با مقاله
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