Inhibition of CCR8 attenuates Ang Ⅱ-induced vascular smooth muscle cell injury by suppressing the MAPK/NF-κB pathway | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 8، دوره 25، شماره 9، آذر 2022، صفحه 1097-1103 اصل مقاله (901.88 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2022.64524.14191 | ||
| نویسندگان | ||
| Lisi Liao؛ Di Song؛ Bobo Shi؛ Ming Chen؛ Linghu Wu؛ Jinfeng Xu* ؛ Fajin Dong* | ||
| The Second Clinical Medical College, Jinan University; Department of Ultrasound, The First Affiliated Hospital, Southern University of Science and Technology; Department of Ultrasound, Shenzhen People’s Hospital, Shenzhen 518020, Guangdong, China | ||
| چکیده | ||
| Objective(s): Hyperinsulinemia, secondary to insulin resistance, may lead to vascular smooth muscle cell dysfunction. In the present research, we aimed to investigate the effect of Chemokine receptor 8 (CCR8) on angiotensin II (Ang II)-induced dysfunction of vascular smooth muscle cells (VSMCs) and to explore the underlying molecular mechanism. Materials and Methods: The expression of CCR8 was analyzed in diabetics and normal people by RT-PCR and ELISA. CCK-8 assay and transwell were used to explore cell proliferation and migration, and ELISA was used to measure the content of IL-6 and TNF-α. Reactive oxygen species (ROS) kit was employed to measure ROS generation. Results: The results revealed that CCR8 was highly expressed in diabetics and Ang Ⅱ-induced VSMCs. Further studies found that interfering with the expression of CCR8 significantly reduced the production of ROS and the levels of inflammatory factors in AngⅡ-induced VSMCs. Interfering with CCR8 increased the glucose uptake induced by AngⅡ+IR. More importantly, inhibition of CCR8 alleviated Ang II-induced dysfunction of VSMCs. Inhibition of CCR8 inactivated the MAPK/NF-κB signaling pathway. Conclusion: Inhibition of CCR8 attenuates Ang II-induced VSMCs injury by inhibiting the MAPK/NF-κB pathway. CCR8 may be a new biomarker related to hypertension and insulin resistance and is a new target for the treatment of human cardiovascular diseases. | ||
| کلیدواژهها | ||
| CCR8؛ Hypertension؛ Inflammation؛ Insulin resistance؛ MAPK/NF-κB signaling؛ Oxidative | ||
| مراجع | ||
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