سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Khorsandi, Layasadat, Orazizadeh, Mahmoud, Bijan Nejad, Darioush, Heidari Moghadam, Abbas, Nejaddehbashi, Freshteh, Asadi Fard, Yousef. (1401). Spleen extracellular matrix provides a supportive microenvironment for β-cell function. , 25(9), 1159-1165. doi: 10.22038/ijbms.2022.65233.14360
Layasadat Khorsandi; Mahmoud Orazizadeh; Darioush Bijan Nejad; Abbas Heidari Moghadam; Freshteh Nejaddehbashi; Yousef Asadi Fard. "Spleen extracellular matrix provides a supportive microenvironment for β-cell function". , 25, 9, 1401, 1159-1165. doi: 10.22038/ijbms.2022.65233.14360
Khorsandi, Layasadat, Orazizadeh, Mahmoud, Bijan Nejad, Darioush, Heidari Moghadam, Abbas, Nejaddehbashi, Freshteh, Asadi Fard, Yousef. (1401). 'Spleen extracellular matrix provides a supportive microenvironment for β-cell function', , 25(9), pp. 1159-1165. doi: 10.22038/ijbms.2022.65233.14360
Khorsandi, Layasadat, Orazizadeh, Mahmoud, Bijan Nejad, Darioush, Heidari Moghadam, Abbas, Nejaddehbashi, Freshteh, Asadi Fard, Yousef. Spleen extracellular matrix provides a supportive microenvironment for β-cell function. , 1401; 25(9): 1159-1165. doi: 10.22038/ijbms.2022.65233.14360

Spleen extracellular matrix provides a supportive microenvironment for β-cell function

Iranian Journal of Basic Medical Sciences
مقاله 16، دوره 25، شماره 9، آذر 2022، صفحه 1159-1165    اصل مقاله (949.59 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.65233.14360
نویسندگان
Layasadat Khorsandi1، 2؛ Mahmoud Orazizadeh1، 2؛ Darioush Bijan Nejad1، 2؛ Abbas Heidari Moghadam3؛ Freshteh Nejaddehbashi1، 2؛ Yousef Asadi Fard* 1، 2
1Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3Department of Anatomical Sciences, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
چکیده
Objective(s): Type 1 diabetes mellitus is a common autoimmune and multifactorial disorder. Researchers have been interested in making a favorable islet-like tissue model for the treatment of diabetes. The main objective of this study was to determine the effects of the spleen extracellular matrix (S-ECM) on the function of the MIN6 cell line (a β-cell model). 
Materials and Methods: In this experimental research, Wistar rat spleens were decellularized by sodium dodecyl sulfate (SDS) and Triton X-100. S-ECM was characterized by histological assessments, scanning electron microscopy, determination of residua DNA, and examination of the mechanical tensile property. Then, MIN6 cells were seeded on S-ECM scaffold. Glucose-stimulated insulin secretion and mRNA expression of insulin-related genes were examined to confirm the function of the cells. 
Results: The main components of S-ECM such as collagen and glycosaminoglycan remained after decellularization. Furthermore, very low residual DNA and appropriate mechanical behavior of S-ECM provided an ideal extracellular microenvironment for the MIN6 cells. GSIS results showed that the seeded cells in S-ECM secreted more insulin than the traditional two-dimensional (2D) culture. The expression of specific insulin-related genes such as PDX-1, insulin, Maf-A, and Glut-2 in the recellularized scaffold was more significant than in the 2D traditional cultured cells. Also, MTT assay results showed that S-ECM were no cytotoxic effects on the MIN6 cells. 
Conclusion: These results collectively have evidenced that S-ECM is a suitable scaffold for stabilizing artificial pancreatic islands. 
کلیدواژه‌ها
Artificial organs؛ Extracellular matrix؛ Insulin؛ Insulin-secreting cells؛ Spleen
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