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Imanparast, Armin, Attaran, Neda, Eshghi, Hossein, Sazgarnia, Ameneh. (1401). Surface modification of gold nanoparticles with 6-mercapto-1-hexanol to facilitate dual conjugation of protoporphyrin IX and folic acid for improving the targeted photochemical internalization. , 25(8), 970-979. doi: 10.22038/ijbms.2022.63622.14033
Armin Imanparast; Neda Attaran; Hossein Eshghi; Ameneh Sazgarnia. "Surface modification of gold nanoparticles with 6-mercapto-1-hexanol to facilitate dual conjugation of protoporphyrin IX and folic acid for improving the targeted photochemical internalization". , 25, 8, 1401, 970-979. doi: 10.22038/ijbms.2022.63622.14033
Imanparast, Armin, Attaran, Neda, Eshghi, Hossein, Sazgarnia, Ameneh. (1401). 'Surface modification of gold nanoparticles with 6-mercapto-1-hexanol to facilitate dual conjugation of protoporphyrin IX and folic acid for improving the targeted photochemical internalization', , 25(8), pp. 970-979. doi: 10.22038/ijbms.2022.63622.14033
Imanparast, Armin, Attaran, Neda, Eshghi, Hossein, Sazgarnia, Ameneh. Surface modification of gold nanoparticles with 6-mercapto-1-hexanol to facilitate dual conjugation of protoporphyrin IX and folic acid for improving the targeted photochemical internalization. , 1401; 25(8): 970-979. doi: 10.22038/ijbms.2022.63622.14033

Surface modification of gold nanoparticles with 6-mercapto-1-hexanol to facilitate dual conjugation of protoporphyrin IX and folic acid for improving the targeted photochemical internalization

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 25، شماره 8، آبان 2022، صفحه 970-979    اصل مقاله (903.44 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.63622.14033
نویسندگان
Armin Imanparast1؛ Neda Attaran* 2؛ Hossein Eshghi3؛ Ameneh Sazgarnia1
1Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2Department of Medical Nanotechnology, Applied Biophotonics Research Center, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
3Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده
Objective(s): Photochemical internalization (PCI) is an important type of photodynamic therapy for delivering macromolecules into the cytosol by the endocytosis process. In this study, 6-mercapto-1-hexanol (MH) was used to functionalize the gold nanostructure as a primer for surface modification to improve conjugation of multi-agents such as protoporphyrin IX (Pp-IX) and folic acid with gold nanoparticles (PpIX/FA-MH-AuNP) to facilitate the photochemical internalization.
Materials and Methods: After surface modification of AuNPs with MH, PpIX and FA are bonded to the surface of the MH-AuNPs through the coupling reaction to produce the desired conjugated AuNPs. In the next step, the synthesized nanostructures were characterized by different methods. Finally, after selecting specific concentrations, light treatments were applied and cell survival was measured based on MTT analysis. Also, in order to better study the morphology of the cells, they were stained by the Giemsa method. The SPSS 16 software was used for data analysis
Results: By surface modification of the nanostructure with MH and then conjugation of FA to it, the incubation time of the drug in PpIX/FA-MH-AuNP was reduced from 3 hr to 30 min. Also, at each light dose, cell death in the presence of PpIX/FA-MH-AuNP was significantly reduced compared with unconjugated conditions (P<0.001). Under these conditions, the ED50 for PpIX and PpIX-MH-AuNP and PpIX/FA-MH-AuNP at a concentration of 2.5 μg/ml is 8.9, 9.1, and 6.17 min, respectively.
Conclusion: The results show that the PCI of PpIX/FA-MH-AuNP increases the selective phototoxicity efficiency on cancer cells compared with the conventional process of photodynamic therapy.
کلیدواژه‌ها
6-mercapto-1-hexanol؛ Cervical cancer؛ Folic acid؛ Gold nanoparticle؛ Photochemical - internalization؛ Photodynamic therapy؛ Protoporphyrin-IX
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