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Hadavi, Mirhossein, H. Najdegerami, Ebrahim, Nikoo, Mehdi, Nejati, Vahid. (1401). Protective effect of protein hydrolysates from Litopenaeus vannamei waste on oxidative status, glucose regulation, and autophagy genes in non-alcoholic fatty liver disease in Wistar rats. , 25(8), 954-963. doi: 10.22038/ijbms.2022.62167.13761
Mirhossein Hadavi; Ebrahim H. Najdegerami; Mehdi Nikoo; Vahid Nejati. "Protective effect of protein hydrolysates from Litopenaeus vannamei waste on oxidative status, glucose regulation, and autophagy genes in non-alcoholic fatty liver disease in Wistar rats". , 25, 8, 1401, 954-963. doi: 10.22038/ijbms.2022.62167.13761
Hadavi, Mirhossein, H. Najdegerami, Ebrahim, Nikoo, Mehdi, Nejati, Vahid. (1401). 'Protective effect of protein hydrolysates from Litopenaeus vannamei waste on oxidative status, glucose regulation, and autophagy genes in non-alcoholic fatty liver disease in Wistar rats', , 25(8), pp. 954-963. doi: 10.22038/ijbms.2022.62167.13761
Hadavi, Mirhossein, H. Najdegerami, Ebrahim, Nikoo, Mehdi, Nejati, Vahid. Protective effect of protein hydrolysates from Litopenaeus vannamei waste on oxidative status, glucose regulation, and autophagy genes in non-alcoholic fatty liver disease in Wistar rats. , 1401; 25(8): 954-963. doi: 10.22038/ijbms.2022.62167.13761

Protective effect of protein hydrolysates from Litopenaeus vannamei waste on oxidative status, glucose regulation, and autophagy genes in non-alcoholic fatty liver disease in Wistar rats

Iranian Journal of Basic Medical Sciences
مقاله 4، دوره 25، شماره 8، آبان 2022، صفحه 954-963    اصل مقاله (640.38 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.62167.13761
نویسندگان
Mirhossein Hadavi1؛ Ebrahim H. Najdegerami* 1؛ Mehdi Nikoo2؛ Vahid Nejati1
1Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
2Department of Pathobiology and Quality Control, Artemia & Aquaculture Research institute, Urmia University, Urmia, Iran
چکیده
Objective(s): The effects of protein hydrolysates (FP) from Litopenaeus vannamei on oxidative stress, and autophagy gene expression was investigated in the NAFLD-induced rats.  
Materials and Methods: For this purpose, twenty-four male rats were divided into four groups: Control, High-fat diet (HFD), FP20+HFD, and FP300+HFD (20 and 300 mg FP /kg rat body weight) and fed for 70 days. 
Results: The results indicated that the rat body and relative weight of the liver were not affected by experimental treatments (P>0.05) although the highest relative weight of the liver was observed in HFD treatment. The highest and lowest values for antioxidant enzymes and MDA concentration were observed in FP treatments (P<0.05). Also, the results showed that FP significantly decreased liver enzymes (ALT, AST) in the liver in comparison with HFD treatment (P<0.05). Plasma biochemical indices were investigated and the lowest amylase, ALP, fasting glucose, insulin, HOMA-IR, triglycerides, cholesterol, and inflammation cytokines (TNF-α, IL-6) were seen in the FP treatments which had a significant difference with HFD (P<0.05). Autophagy gene expression in the liver cells was affected by experimental diets and the lowest expression of Beclin-1 and Atg7 was observed in HFD and FP300 treatments. Interestingly, the highest expression of LC3-ɪ and P62 was seen in HFD and FP treatments, not in the control. 
Conclusion: Overall, the results of this experiment indicated that FPs extracted from Whiteleg shrimp at 50 °C improve the oxidative status, glucose metabolism, and autophagy gene expression and could be used as a useful nutritional strategy in fatty liver prevention.
کلیدواژه‌ها
Autophagy؛ Bioactive peptides؛ Fatty liver؛ Insulin؛ Oxidative enzymes؛ Whiteleg shrimp
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