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Yuan, Qing, Wang, Jing, Guo, Lichen, Xu, Yao, Hu, Limin, Mao, Haoping, Miao, Lin, Zhang, Han, Chai, Lijuan. (1401). Neobavaisoflavone ameliorates LPS-induced RAW264.7 cell inflammations by suppressing the activation of NF-κB and MAPKs signaling pathways. , 25(8), 1021-1027. doi: 10.22038/ijbms.2022.65372.14389
Qing Yuan; Jing Wang; Lichen Guo; Yao Xu; Limin Hu; Haoping Mao; Lin Miao; Han Zhang; Lijuan Chai. "Neobavaisoflavone ameliorates LPS-induced RAW264.7 cell inflammations by suppressing the activation of NF-κB and MAPKs signaling pathways". , 25, 8, 1401, 1021-1027. doi: 10.22038/ijbms.2022.65372.14389
Yuan, Qing, Wang, Jing, Guo, Lichen, Xu, Yao, Hu, Limin, Mao, Haoping, Miao, Lin, Zhang, Han, Chai, Lijuan. (1401). 'Neobavaisoflavone ameliorates LPS-induced RAW264.7 cell inflammations by suppressing the activation of NF-κB and MAPKs signaling pathways', , 25(8), pp. 1021-1027. doi: 10.22038/ijbms.2022.65372.14389
Yuan, Qing, Wang, Jing, Guo, Lichen, Xu, Yao, Hu, Limin, Mao, Haoping, Miao, Lin, Zhang, Han, Chai, Lijuan. Neobavaisoflavone ameliorates LPS-induced RAW264.7 cell inflammations by suppressing the activation of NF-κB and MAPKs signaling pathways. , 1401; 25(8): 1021-1027. doi: 10.22038/ijbms.2022.65372.14389

Neobavaisoflavone ameliorates LPS-induced RAW264.7 cell inflammations by suppressing the activation of NF-κB and MAPKs signaling pathways

Iranian Journal of Basic Medical Sciences
مقاله 13، دوره 25، شماره 8، آبان 2022، صفحه 1021-1027    اصل مقاله (961.59 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.65372.14389
نویسندگان
Qing Yuan1، 2؛ Jing Wang3؛ Lichen Guo1، 2؛ Yao Xu1، 2؛ Limin Hu1، 2؛ Haoping Mao1، 2؛ Lin Miao1، 2؛ Han Zhang* 1، 2؛ Lijuan Chai* 1، 2
1State Key Laboratory of Component-based Chinese Medicine, Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, China
2Tianjin Key Laboratory of Traditional Chinese medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin, China
3China Resources Sanjiu Medical & Pharmaceutical Co., Ltd, Shenzhen, China
چکیده
Objective(s): Neobavaisoflavone (NBIF) is an isoflavone isolated from Psoralea corylifolia L. It can effectively regulate the redox state as a natural anti-oxidant and show some anti-inflammatory activity. However, its molecular mechanism is poorly studied. In this study, RAW264.7 cells were treated with lipopolysaccharide (LPS) to investigate the anti-inflammatory activity and potential NBIF mechanism.
Materials and Methods: RAW264.7 cells were treated with LPS (62.5 ng/ml) and exposed to different concentrations of NBIF (0.01, 0.1, and 1 μM) for 24 hr. Inflammatory cytokines of RAW264.7 cells were measured by the Griess method, ELISA, and western blot. Phagocytosis of RAW264.7 macrophages was measured by FITC-dextran uptake assay. The phosphorylation protein expression levels of MAPKs (JNK, p38, and ERK), NF-κB p65, IκBα, and IκB kinase were analyzed by western blot. The results were analyzed using one-way ANOVA with Tukey’s multiple comparison test.
Results: NBIF significantly inhibited NO and ROS production by down-regulation of iNOS and COX-2 protein expression. Additionally, the amount of release and protein levels of inflammation cytokines IL-6, IL-1β, and TNF-α were significantly decreased by NBIF. Moreover, FITC–dextran uptake assay by flow cytometry presented that NBIF significantly enhanced the phagocytic capacity of RAW264.7. Mechanistically, NBIF significantly down-regulated MAPK activation and inhibited the nuclear translocation of NF-κB p65. 
Conclusion: The present study demonstrates that NBIF inhibited inflammation and enhanced the phagocytic capacity of RAW264.7 cell-related MAPKs and NF-κB signaling pathways induced by LPS. These findings suggest that NBIF may have clinical utility as an anti-inflammatory agent.
کلیدواژه‌ها
Inflammation؛ MAP kinase signaling - system؛ Neobavaisoflavone؛ NF-kappa B
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