Rezakhani, Nakisa, Goliaei, Bahram, Mahdizade, Fereshte, Nikoofar, Alireza, Keshmiri Neghab, Hoda, Soheilifar, -Mohammad Hasan, Azadeh, Seyedeh Sara, Bigdeli, Bahareh. (1401). Evaluation of Sinensetin Derived From Flavonoids-Rich Orthosiphon Stamineus as a New Radiosensitizer in MDA-MB-231 and T47D as Breast Cancer Cell Lines. , 20(2), 114-119. doi: 10.22038/ijmp.2022.64397.2104
Nakisa Rezakhani; Bahram Goliaei; Fereshte Mahdizade; Alireza Nikoofar; Hoda Keshmiri Neghab; -Mohammad Hasan Soheilifar; Seyedeh Sara Azadeh; Bahareh Bigdeli. "Evaluation of Sinensetin Derived From Flavonoids-Rich Orthosiphon Stamineus as a New Radiosensitizer in MDA-MB-231 and T47D as Breast Cancer Cell Lines". , 20, 2, 1401, 114-119. doi: 10.22038/ijmp.2022.64397.2104
Rezakhani, Nakisa, Goliaei, Bahram, Mahdizade, Fereshte, Nikoofar, Alireza, Keshmiri Neghab, Hoda, Soheilifar, -Mohammad Hasan, Azadeh, Seyedeh Sara, Bigdeli, Bahareh. (1401). 'Evaluation of Sinensetin Derived From Flavonoids-Rich Orthosiphon Stamineus as a New Radiosensitizer in MDA-MB-231 and T47D as Breast Cancer Cell Lines', , 20(2), pp. 114-119. doi: 10.22038/ijmp.2022.64397.2104
Rezakhani, Nakisa, Goliaei, Bahram, Mahdizade, Fereshte, Nikoofar, Alireza, Keshmiri Neghab, Hoda, Soheilifar, -Mohammad Hasan, Azadeh, Seyedeh Sara, Bigdeli, Bahareh. Evaluation of Sinensetin Derived From Flavonoids-Rich Orthosiphon Stamineus as a New Radiosensitizer in MDA-MB-231 and T47D as Breast Cancer Cell Lines. , 1401; 20(2): 114-119. doi: 10.22038/ijmp.2022.64397.2104
Evaluation of Sinensetin Derived From Flavonoids-Rich Orthosiphon Stamineus as a New Radiosensitizer in MDA-MB-231 and T47D as Breast Cancer Cell Lines
1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
3Department of Biology, Islamshahr Branch, Islamic Azad University, Tehran, Iran
4Department of Radiotherapy, Iran University of Medical Sciences (IUMS), Tehran, Iran
5Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
6Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, IRAN
7Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
چکیده
Introduction: Breast cancer is one of the most prevalent diseases around the world. Breast cancer patients treated with radiation may face Side effects as well as cancer recurrence. Some polyphenols exhibit antioxidant effects. Citrus and Orthosiphon Stamineus have source of methylated flavone called Sinensetin (SIN). In this research, we designation of Sinensetin as increasing radiation sensitivity. Material and Methods: The cytotoxic effect of Sinensetin was examined in MDA-MB-231 and T47D by MTT assay. As well as, the clonogenic ability of cells were Assessmented in the presence of Sinensetin and combination with radiation. To quantify expression alterations of apoptosis related genes, utilized Real-Time PCR method. Results: In a dose and time-dependent manner, Sinensetin decreased the viability of MDA-MB-231 and T47D. The survival fraction was decreased in cells treated with Sinensetin (SIN) prior to X-irradiation compared to cells treated with X-ray only. More ever, expression level of, Bcl-2, STAT3, and increased P53 via treated cells with Sinensetin (SIN) and X-ray. Conclusion: Due to the results, Sinensetin (SIN) can be mentioned as a novel radiosensitizer and its effects may considered increasing apoptosis following DNA damage induced by irradiation.
Jones S, Hogan B, Patel K, Ooi S, Turton P, Achuthan R, et al. Identification of factors that influence the decision to take chemoprevention in patients with a significant family history of breast cancer: results from a patient questionnaire survey. Breast Cancer Research and Treatment. 2021;187(1):207-13.
Mohd Ali N, Yeap SK, Ho WY, Boo L, Ky H, Satharasinghe DA, et al. Adipose MSCs suppress MCF7 and MDA-MB-231 breast cancer metastasis and EMT pathways leading to dormancy via exosomal-miRNAs following co-culture interaction. Pharmaceuticals. 2020;14(1):8.
Cao K, Abbassi L, Romano E, Kirova Y. Radiation therapy and immunotherapy in breast cancer treatment: preliminary data and perspectives. Expert Review of Anticancer Therapy. 2021;21(5):501-10.
Sato Y, Yoshino H, Kashiwakura I, Tsuruga E. DAP3 is involved in modulation of cellular radiation response by RIG-I-like receptor agonist in human lung adenocarcinoma cells. International Journal of Molecular Sciences. 2021;22(1):420.
Budanov AV. The role of tumor suppressor p53 in the antioxidant defense and metabolism. Mutant p53 and MDM2 in Cancer. 2014:337-58.
Zinkel S, Gross A, Yang E. BCL-2 family in DNA damage and cell cycle control. Cell Death & Differentiation. 2006;13(8):1351-9.
Kortylewski M, Yu H. Role of Stat3 in suppressing anti-tumor immunity. Current opinion in immunology. 2008;20(2):228-33.
van Vuuren RJ, Visagie MH, Theron AE, Joubert AM. Antimitotic drugs in the treatment of cancer. Cancer chemotherapy and pharmacology. 2015;76(6):1101-12.
Li S, Wu H, Tollefsbol TO. Combined broccoli sprouts and green tea polyphenols contribute to the prevention of estrogen receptor–negative mammary cancer via cell cycle arrest and inducing apoptosis in HER2/neu mice. The Journal of Nutrition. 2021;151(1):73-84.
Poggi MM, Coleman CN, Mitchell JB. Sensitizers and protectors of radiation and chemotherapy. Current problems in cancer. 2001;25(6):334-411.
Hossain MA, Ismail Z. Quantification and enrichment of sinensetin in the leaves of Orthosiphon stamineus. Arabian Journal of Chemistry. 2016;9:S1338-S41.
Samidurai D, Pandurangan AK, Krishnamoorthi SK, Perumal MK, Nanjian R. Sinensetin isolated from Orthosiphon aristatus inhibits cell proliferation and induces apoptosis in hepatocellular carcinoma cells. Process Biochemistry. 2020;88:213-21.
Bigdeli B, Goliaei B, Masoudi-Khoram N, Jooyan N, Nikoofar A, Rouhani M, et al. Enterolactone: A novel radiosensitizer for human breast cancer cell lines through impaired DNA repair and increased apoptosis. Toxicology and applied pharmacology. 2016;313:180-94.
Khoram NM, Bigdeli B, Nikoofar A, Goliaei B. Caffeic acid phenethyl ester increases radiosensitivity of estrogen receptor-positive and-negative breast cancer cells by prolonging radiation-induced DNA damage. Journal of breast cancer. 2016;19(1):18-25.
Taghizadeh B, Ghavami L, Nikoofar A, Goliaei B. Equol as a potent radiosensitizer in estrogen receptor-positive and-negative human breast cancer cell lines. Breast Cancer. 2015;22(4):382-90.
Keshmiri-Neghab H, Goliaei B, Nikoofar A. Gossypol enhances radiation induced autophagy in glioblastoma multiforme. Gen Physiol Biophys. 2014;33(4):433-42.
Movahedi A, Basir R, Rahmat A, Charaffedine M, Othman F. Orthosiphon stamineus: an Asian tea with substantial anticancer properties. Journal of Nutritional Sciences and Dietetics. 2015:44-52.
Pauzi N, Mohd KS, Halim NHA, Ismail Z. Orthosiphon stamineus extracts inhibits proliferation and induces apoptosis in uterine fibroid cells. Asian Pacific Journal of Cancer Prevention: APJCP. 2018;19(10):2737.
Dienaitė L, Pukalskas A, Pukalskienė M, Pereira CV, Matias AA, Venskutonis PR. Phytochemical composition, antioxidant and antiproliferative activities of defatted sea buckthorn (Hippophaë rhamnoides L.) berry pomace fractions consecutively recovered by pressurized ethanol and water. Antioxidants. 2020;9(4):274.
Al-Sadi AM, Al-Oweisi FA, Edwards SG, Al-Nadabi H, Al-Fahdi AM. Genetic analysis reveals diversity and genetic relationship among Trichoderma isolates from potting media, cultivated soil and uncultivated soil. BMC microbiology. 2015;15(1):1-11.
Mahdizade VF, Goliaei B, Parivar K, Nikoofar A. Effect of a Neoflavonoid (Dalbergin) on T47D Breast Cancer Cell Line and mRNA Levels of p53, Bcl-2, and STAT3 Genes. 2019.
