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Wen, Mingxiao, Chen, Wenjun, Zhou, Qun, Dou, Xiaoqing. (1401). Astragaloside IV regulates autophagy-mediated proliferation and apoptosis in a rat model of PCOS by activating the PPARγ pathway. , 25(7), 882-889. doi: 10.22038/ijbms.2022.64475.14179
Mingxiao Wen; Wenjun Chen; Qun Zhou; Xiaoqing Dou. "Astragaloside IV regulates autophagy-mediated proliferation and apoptosis in a rat model of PCOS by activating the PPARγ pathway". , 25, 7, 1401, 882-889. doi: 10.22038/ijbms.2022.64475.14179
Wen, Mingxiao, Chen, Wenjun, Zhou, Qun, Dou, Xiaoqing. (1401). 'Astragaloside IV regulates autophagy-mediated proliferation and apoptosis in a rat model of PCOS by activating the PPARγ pathway', , 25(7), pp. 882-889. doi: 10.22038/ijbms.2022.64475.14179
Wen, Mingxiao, Chen, Wenjun, Zhou, Qun, Dou, Xiaoqing. Astragaloside IV regulates autophagy-mediated proliferation and apoptosis in a rat model of PCOS by activating the PPARγ pathway. , 1401; 25(7): 882-889. doi: 10.22038/ijbms.2022.64475.14179

Astragaloside IV regulates autophagy-mediated proliferation and apoptosis in a rat model of PCOS by activating the PPARγ pathway

Iranian Journal of Basic Medical Sciences
مقاله 12، دوره 25، شماره 7، مهر 2022، صفحه 882-889    اصل مقاله (1.37 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.64475.14179
نویسندگان
Mingxiao Wen1؛ Wenjun Chen2؛ Qun Zhou2؛ Xiaoqing Dou* 2
1Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Hangzhou 310000, China
2Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Hangzhou 310000, China
چکیده
Objective(s): Astragaloside IV (AS-IV) is a bioactive saponin with a wide range of pharmacological effects. This study was aimed at investigating its potential effect on polycystic ovary syndrome (PCOS). 
Materials and Methods: Female Sprague-Dawley rats were randomly divided into five groups (control, PCOS, PCOS+AS-IV 20 mg/kg, PCOS+AS-IV 40 mg/kg, and PCOS+AS-IV 80 mg/kg). The pathological injury level of rat ovary was observed with hematoxylin-eosin (H&E) staining; enzyme-linked immunosorbent assay (ELISA) kit was utilized to measure the levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone in rat serum; western blot detected autophagy-associated or peroxisome proliferator-activated receptor γ (PPARγ) pathway-related protein expression; immunofluorescence was performed to observe LC3 level in rat ovarian tissue. After co-treatment with AS-IV and PPARγ inhibitor, the proliferation in ovarian granulosa cell line KGN was examined employing cell counting kit-8 (CCK-8), EdU staining, and colony formation; cell apoptosis was observed with TdT-mediated dUTP nick-end labeling (TUNEL); apoptosis-related protein expression was assayed by western blot. 
Results: Treatment with AS-IV inhibited the ovarian pathological damage in PCOS rats. It also promoted the level of autophagy and activated PPARγ signaling in the rat PCOS model. In KGN cells, the level of autophagy and expression of PPARγ-related proteins were also elevated by AS-IV treatment. Furthermore, AS-IV facilitated autophagy, thus inhibiting KGN cell proliferation and promoting its apoptosis, through activating the PPARγ signaling pathway. 
Conclusion: AS-IV-activated PPARγ inhibits proliferation and promotes the apoptosis of ovarian granulosa cells, enhancing ovarian function in rats with PCOS.
کلیدواژه‌ها
Apoptosis؛ Astragaloside IV؛ Autophagy؛ Polycystic ovary syndrome؛ PPARγ؛ Rat
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