سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Hooshangi, Pegah, Kazemzadeh, Yaser, Shirvani, Hossein, Sedaghati, Saeid, Molanoruzi, Keyvan. (1401). The Effect of Nanoselenium Consumption during High Intensity Interval Training on IL-4 and IFN-γ Gene Expression in Thymus Organ of Dexamethasone-Induced Immunosuppressive Rats. , 10(2), 129-135. doi: 10.22038/jnfh.2022.65454.1387
Pegah Hooshangi; Yaser Kazemzadeh; Hossein Shirvani; Saeid Sedaghati; Keyvan Molanoruzi. "The Effect of Nanoselenium Consumption during High Intensity Interval Training on IL-4 and IFN-γ Gene Expression in Thymus Organ of Dexamethasone-Induced Immunosuppressive Rats". , 10, 2, 1401, 129-135. doi: 10.22038/jnfh.2022.65454.1387
Hooshangi, Pegah, Kazemzadeh, Yaser, Shirvani, Hossein, Sedaghati, Saeid, Molanoruzi, Keyvan. (1401). 'The Effect of Nanoselenium Consumption during High Intensity Interval Training on IL-4 and IFN-γ Gene Expression in Thymus Organ of Dexamethasone-Induced Immunosuppressive Rats', , 10(2), pp. 129-135. doi: 10.22038/jnfh.2022.65454.1387
Hooshangi, Pegah, Kazemzadeh, Yaser, Shirvani, Hossein, Sedaghati, Saeid, Molanoruzi, Keyvan. The Effect of Nanoselenium Consumption during High Intensity Interval Training on IL-4 and IFN-γ Gene Expression in Thymus Organ of Dexamethasone-Induced Immunosuppressive Rats. , 1401; 10(2): 129-135. doi: 10.22038/jnfh.2022.65454.1387

The Effect of Nanoselenium Consumption during High Intensity Interval Training on IL-4 and IFN-γ Gene Expression in Thymus Organ of Dexamethasone-Induced Immunosuppressive Rats

Journal of Nutrition,Fasting and Health
مقاله 7، دوره 10، شماره 2، شهریور 2022، صفحه 129-135    اصل مقاله (1.13 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/jnfh.2022.65454.1387
نویسندگان
Pegah Hooshangi1؛ Yaser Kazemzadeh* 1؛ Hossein Shirvani2؛ Saeid Sedaghati1؛ Keyvan Molanoruzi1
1Department of Sport Physiology, Faculty of Physical Education, Islamic Azad University, Islamshahr Branch, Islamshahr, Iran.
2Associate Professor of Exercise Physiology Exercise Physiology Research Center Life Style Institute Baqiyatallah University of Medical Sciences, Tehran, Iran.
چکیده
Introduction: The thymus is a specialized lymphatic organ in the immune system and plays a vital role in the normal functioning of the immune system. Observed 24 and 48 hours after dexamethasone, thymocytes are reduced by 55% and 84%, respectively, which can be associated with a decrease in anti-inflammatory cytokines such as IL4 and IFN-γ. The aim of present study was to evaluate the The effect of nanoselenium consumption during high intensity interval training on IL-4 and IFN-γ gene expression in thymus organ of dexamethasone-induced immunosuppressive rats. Methods: The study samples in the present study consisted of 40 male Wistar rats that were randomly divided into 5 groups: healthy control group (CON), immunosuppression group (DEX), immunosuppression + exercise group (DEX + TRA), immunosuppression group + nanosillenium (DEX + SEL), immunosuppression group + nanoselnium + training (DEX + TRA + SEL) were divided. Suppression of the immune system of the samples was performed by injecting of 0.4 mg / kg per day dexamethasone for three days. The training program included 4 weeks of intense intermittent training (HIIT) in the DEX + TRA and DEX + TRA + SEL groups, and supplementation with 100 mg / kg selenium nanoparticles in the DEX + SEL and DEX + TRA + SEL groups. Data were analyzed using one way ANOVA in SPSS 26 software at significance level of α <0.05. Results: The data showed that IFN-γ gene expression decreased in all groups compared to the control group (p = 0.0001). The difference between DEX + SEL and DEX + SEL + TRA groups was less than other groups. Also, IL4 gene expression in thymus tissue was significantly reduced in DEX and DEX + TRA groups compared to healthy controls (p = 0.048 and p = 0.013, respectively). Conclusion: In the present study, it was found that intense exercise activity in high-intensity interval training (HIIT) may inhibit immune reactions and anti-inflammatory cytokines in the thymus tissue of rats whose immune system was suppressed by DEX. Therefore, exercise for strengthening the immune system in these people should be done with more caution.
کلیدواژه‌ها
Nanoselnium؛ IL-4, IFN-γ؛ Dexamethasone؛ Interval training
مراجع
  1. Avery JC, Hoffmann PR. Selenium, Selenoproteins, and Immunity. Nutrients. 2018 1;10(9):1203.
  2. Chen L, Jondal M, Yakimchuk K. Regulatory effects of dexamethasone on NK and T cell immunity. Inflammopharmacology. 2018; 26(5):1331-8.
  3. Cheng WH, Holmstrom A, Li X, Wu RT, Zeng H, Xiao Z. Effect of dietary selenium and cancer cell xenograft on peripheral T and B lymphocytes in adult nude mice. Biol Trace Elem Res. 2012;146:230-5.
  4. Castro F, Cardoso AP, Gonçalves RM, Serre K, Oliveira MJ. Interferon-Gamma at the Crossroads of Tumor Immune Surveillance or Evasion. Front Immunol. 2018; 9:847.
  5. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Preventive Cardiology. 2007;14(6):753-60.
  6. Hoffmann FW, Hashimoto AC, Shafer LA, Dow S, Berry MJ, Hoffmann PR. Dietary selenium modulates activation and differ- entiation of CD4+ T-cells in mice through a mechanism involving cellular free thiols. J Nutr. 2010; 140:1155-61.
  7. Hawkes WC, Kelley DS, Taylor PC. The effects of dietary selenium on the immune system in healthy men. Biol Trace Ele 2001; 81:189-213.
  8. Hussar P, Tokin I, Hussar U, Filimonova G, Suuroja T. Application of Photoshop-based image analysis and TUNEL for the distri- bution and quantification of dexametha- sone-induced apoptotic cells in rat thymus. Medicina (Kaunas). 2006; 42:504-12.
  9. Ito SH. High-intensity interval training for health benefits and care of cardiac diseases - The key to an efficient exercise protocol. World J Cardiol. 2019; 11(7): 171-188.
  10. Jameson SC, Lee YJ, Hogquist KA. Innate Memory T Cells. Adv Immunol (2015) 126:173–213.
  11. Jeklova E, Leva L, Jaglic Z, Faldyna M. Dexamethasone-induced immunosuppression: a rabbit model. Vet Immunol Immu- nopathol. 2008; 122:231-40.
  12. Khorasani Gh, Hosseini M , Divkan B , Riyahi Malayeri  Interactive Effect of High Intensity Interval Training with Vitamin E Consumption on the Serum Levels of Hsp70 and SOD in Male Wistar Rats. Iran J Nutr Sci Food Technol. 2019, 13(4), 21-28.
  13. Kiremidjian-Schumacher L, Roy M. Effect of selenium on the immunocompetence of patients with head and neck cancer and on adoptive immunotherapy of early and estab- lished lesions. Biofactors. 2001; 14(1-4):161
  14. Kojouri GA, Sadeghian S, Mohebbi A, Dezfouli MRM. The effects of oral consumption of selenium nanoparticles on chemotactic and respiratory burst activities of neutrophils in comparison with sodium selenite in sheep. Biol Trace Elem Res. 2012; 146(2):160-6.
  15. Khurana A, Tekula S, Saifi MA, Venkatesh P, Godugu C. Therapeutic applications of selenium nanoparticles. Biomed Pharmacother. 2019; 111:802-12.
  16. Little JP, Safdar A, Wilkin GP, Tarnopolsky MA, Gibala MJ. A practical model of low‐volume high‐intensity interval training induces mitochondrial biogenesis in human skeletal muscle: potential mechanisms. J Physiol. 2010; 588(6):1011-22.
  17. Nunes-Cabac¸o H, Ramalho-dos-Santos A, Pires AR, Martins LR, Barata JT, Sousa AE. Human CD4 T Cells From Thymus and Cord Blood Are Convertible Into CD8 T Cells by IL-4. Immunol. 2022; 13:834033.
  18. Oliver JA, Stolberg VR, Chensue SW, King PD. IL-4 acts as a potent stimulator of IFN-g expression in CD8+ T cells through STAT6- dependent and independent induction of eomesodermin and T-Bet. 2012; 57:191–9.
  19. Papp G, Szabo´ K, Ja´ mbor I, Mile M, Berki AR, Arany AC, Makra G, Szodoray P, Csiki Z, Balogh L. Regular exercise may restore certain age-related alterations of adaptive immunity and rebalance. Immune Regulation Front Immunol. 2021; 12:639308.
  20. Pearse G. Normal structure, function and histology of the thymus. Toxi- col Pathol. 2006; 34(5):504-14.
  21. Radomska D, Czarnomysy R, Radomski D, Bielawska A, Bielawski K. Selenium as a bioactive micronutrient in the human diet and its cancer chemopreventive activity. Nutrients. 2021; 13(5): 1649.
  22. Saeedy M, Bijeh N, Moazzami M. The effect of six weeks of high-intensity interval training with zinc supplementation on some humoral immunity markers in female futsal players. Ann Appl Sport Sci. 2018; 6(1):11-9.
  23. Salimian J, Arefpour MA, Riazipour M, Poursasan N. Immunomodulatory effects of selenium and vitamin E on alterations in T lymphocyte subsets induced by T-2 toxin. Immunopharmacol Immunotoxicol. 2014. 36(4):275-81.
  24. Simpson RJ, Lowder TW, Spielmann G, Bigley AB, LaVoy EC, Kunz H. Exercise and the aging immune system. Ageing Res Rev. 2012; 11:404–20.
  25. Vernec A, Slack A, Harcourt PR, Budgett R, Duclos M, Kinahan A, et al. Glucocorticoids in elite sport: current status, controversies and innovative management strategies-a narrative review. Br J Sports Med. 2020; 54(1):8-12.
  26. Wang W, Thomas R, Sizova O, Su DM. Thymic function associated with cancer development, relapse, and antitumor immunity - a mini-review. Front Immunol. 2020; 11:773.
  27. Wijesundara DK, Tscharke DC, Jackson RJ, Ranasinghe C. reduced interleukin-4 receptor a expression on cd8+ t cells correlates with higher quality anti-viral immunity. PloS One. 2013; 8 (1):e55788
  28. Yazdi MH, Mahdavi M, Faghfuri E, Faramarzi MA, Sepehrizadeh Z, Hassan ZM, et al. Th1 immune response induction by biogenic selenium nanoparticles in mice with breast cancer: preliminary vaccine model. Iran J Biotechnol. 2015;13(2):1.
  29. Yang WC, Hwang YS, Chen YY, Liu CL, Shen CN, Hong WH, Lo SM, Shen CR. Interleukin-4 Supports the Suppressive Immune Responses Elicited by Regulatory T Cells. Front Immunol. 2017; 8:1508.
  30. Zhang S-Y, Zhang J, Wang H-Y, Chen H-Y. Synthesis of selenium nanoparticles in the presence of polysaccharides. Mater Lett. 2004;58(21):2590-4.
آمار
تعداد مشاهده مقاله: 1,404
تعداد دریافت فایل اصل مقاله: 1,042