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Hassan, snur, Saeed, Azad K, Rahim, Omed Omer, Mahmood, Shler A F. (1401). Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine. , 25(7), 897-903. doi: 10.22038/ijbms.2022.65427.14395
snur Mohammad Amen Hassan; Azad K Saeed; Omed Omer Rahim; Shler A F Mahmood. "Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine". , 25, 7, 1401, 897-903. doi: 10.22038/ijbms.2022.65427.14395
Hassan, snur, Saeed, Azad K, Rahim, Omed Omer, Mahmood, Shler A F. (1401). 'Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine', , 25(7), pp. 897-903. doi: 10.22038/ijbms.2022.65427.14395
Hassan, snur, Saeed, Azad K, Rahim, Omed Omer, Mahmood, Shler A F. Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine. , 1401; 25(7): 897-903. doi: 10.22038/ijbms.2022.65427.14395

Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine

Iranian Journal of Basic Medical Sciences
مقاله 14، دوره 25، شماره 7، مهر 2022، صفحه 897-903    اصل مقاله (1.81 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.65427.14395
نویسندگان
snur Mohammad Amen Hassan* 1؛ Azad K Saeed1؛ Omed Omer Rahim2؛ Shler A F Mahmood3
1Department of Anatomy and Histopathology, College of Veterinary Medicine, Sulaimani University, Sulaimani, 4601, KRG, Iraq
2Department of Food Science and Quality Control, Bakrajo Technical Institute, Sulaimani Polytechnic University, Sulaimani, 4601, KRG, Iraq
3Department of Microbiology, School of Medicine, Sulaimani University, Sulaimani, 4601, KRG, Iraq
چکیده
Objective(s): To assess the protective effect of L-carnitine in reducing cisplatin toxicity via estimating biochemical tests, histomorphometric, and immunohistochemistry (IHC) of β-catenin and cyclin D.
Materials and Methods: Fifteen adult male rabbits were used in this study and allocated into 3 groups; Group 1 (Control negative), rabbits of this group were not given any treatment. In group 2, the animals were injected with cisplatin single-dose/per week. Group 3 rabbits were treated with Cisplatin+L-carnitine orally by gavage tube for 29 days. At the end of the experiments, blood samples from all rabbits were taken from the earlobe, and then the biochemical test was done, the kidney and tissue sections were prepared for both H& E and IHC for both β-catenin and cyclin D genes.
Results: Treatment with L-carnitine reduced the injury effect of cisplatin via a decline in serum creatinine, urea, bilirubin, GPT, GOP, and ALP significantly (P<0.05). Also, administration of LC attenuates the histopathologic abnormality in the kidney (15.71% vs 85.18%) and liver (score 3 vs 15 ) induced by cisplatin. L-carnitine elevates the expression of β-catenin and cyclin D in renal and hepatic parenchyma by diffuse, moderate-strong positivity vs cisplatin that showed local-weak staining.
Conclusion: These findings imply that L-carnitine, by its pleiotropic actions in activating Wnt signaling, alleviates cisplatin-induced renal and hepatic destruction. It might be a method of preventing cisplatin-related nephrotoxicity and hepatotoxicity.
کلیدواژه‌ها
β-catenin؛ Cisplatin؛ Cyclin D؛ Hepatic regeneration؛ L-carnitine؛ Tubular necrosis
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