Neuroprotective effects of the fractions of Ocimum basilicum in seizures induced by pentylenetetrazole in mice | ||
Avicenna Journal of Phytomedicine | ||
دوره 12، شماره 6، بهمن و اسفند 2022، صفحه 614-626 اصل مقاله (1.28 M) | ||
نوع مقاله: Original Research Article | ||
شناسه دیجیتال (DOI): 10.22038/ajp.2022.20470 | ||
نویسندگان | ||
Somaieh Mansouri1؛ Mahmoud Hosseini* 2؛ Fatemeh Alipour3؛ Farimah Beheshti4؛ Hassan Rakhshandeh5؛ Abbas Mohammadipour6؛ Akbar Anaeigoudari* 7؛ Mohammad Jalili-nik8؛ Mohammad Reza Khazdair9؛ Amirali Jahani10 | ||
1Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Department of Anatomy, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran | ||
2Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
3Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
4Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran Department of Physiology, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran | ||
5Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran Science, Mashhad, Iran. | ||
6Department of Anatomy, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
7Department of Physiology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran | ||
8Department of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
9Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran | ||
10Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran | ||
چکیده | ||
Objective: Neuroprotective and antioxidant effects of Ocimum basilicum (O. basilicum) against pentylenetetrazole (PTZ)-induced seizures were investigated. Materials and Methods: Mice were divided as follows: (Group 1) Control, (Group 2) PTZ, (Groups 3-5) 50,100 and 200 mg/kg hydro-ethanolic (HE) extract, and (Groups 6-8) 200 mg/kg ethyl-acetate (EAF), N-hexane (NHF) and water (WF) fractions. Minimal clonic seizures (MCS) and generalized tonic-clonic seizures (GTCS) latencies were measured. Biochemical and histological studies were done. Results: MCS and GTCS latency in HE groups were longer than the PTZ group (p<0.05 to p<0.001). EAF and NHF prolonged the onset of MCS and GTCS (p<0.001). PTZ increased malondialdehyde (MDA) and dark neuron (DN) production while decreased thiol, catalase (CAT) and superoxide dismutase (SOD) (p<0.05 to p<0.001). Pre-treatment by HE and all fractions of the plant attenuated MDA and DN while increased thiol, CAT and SOD (p<0.01 to p<0.001). Conclusion: EAF and NHF had anticonvulsant properties. The extract and fractions protected the brain from PTZ-induced oxidative damages and showed neuroprotective effects. | ||
کلیدواژهها | ||
Neuroprotective؛ Ocimum basilicum؛ Oxidative stress؛ Pentylenetetrazole؛ Seizures | ||
مراجع | ||
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