Mao, Anyun, Qin, Qinghong, Yang, Weiping, Wei, Changyuan. (1401). Synergistic anticancer mechanisms of curcumol and paclitaxel in triple-negative breast cancer treatment may involve down-regulating ZBTB7A expression via the NF-B signaling pathway. , 25(5), 652-658. doi: 10.22038/ijbms.2022.64571.14218
Anyun Mao; Qinghong Qin; Weiping Yang; Changyuan Wei. "Synergistic anticancer mechanisms of curcumol and paclitaxel in triple-negative breast cancer treatment may involve down-regulating ZBTB7A expression via the NF-B signaling pathway". , 25, 5, 1401, 652-658. doi: 10.22038/ijbms.2022.64571.14218
Mao, Anyun, Qin, Qinghong, Yang, Weiping, Wei, Changyuan. (1401). 'Synergistic anticancer mechanisms of curcumol and paclitaxel in triple-negative breast cancer treatment may involve down-regulating ZBTB7A expression via the NF-B signaling pathway', , 25(5), pp. 652-658. doi: 10.22038/ijbms.2022.64571.14218
Mao, Anyun, Qin, Qinghong, Yang, Weiping, Wei, Changyuan. Synergistic anticancer mechanisms of curcumol and paclitaxel in triple-negative breast cancer treatment may involve down-regulating ZBTB7A expression via the NF-B signaling pathway. , 1401; 25(5): 652-658. doi: 10.22038/ijbms.2022.64571.14218

Synergistic anticancer mechanisms of curcumol and paclitaxel in triple-negative breast cancer treatment may involve down-regulating ZBTB7A expression via the NF-B signaling pathway

Iranian Journal of Basic Medical Sciences
مقاله 14، دوره 25، شماره 5، مرداد 2022، صفحه 652-658    اصل مقاله (759.37 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.64571.14218
نویسندگان
Anyun Mao1؛ Qinghong Qin2؛ Weiping Yang2؛ Changyuan Wei* 2
1Department of Thyroid and Breast Surgery, Guangdong Medical University Affiliated Houjie Hospital, Dongguan 523945, People’s Republic of China
2Department of Breast Surgery, Guangxi Medical University Cancer Hospital, Nanning 530021, People’s Republic of China
چکیده
Objective(s): This study aimed to verify whether curcumol combined with paclitaxel exerted synergistic antiproliferative and proapoptotic effects in MDA-MB-231 mammary cancer cells.
Materials and Methods: The effects of different concentrations of CC, PTX, and their combination on the proliferation of MDA-MB-231 mammary cancer cells were determined by CCK-8 laboratory tests. Combination index (CI) was calculated using CompuSyn software. Colony formation assays, Hoechst 33258 immunofluorescence staining, and flow cytometry were carried out to observe proliferation and apoptosis in each group. The protein expression of PCNA, Bcl-2, Bax, ZBTB7A, p-p65, and NF-ƙB p65 was detected by western blotting. The xenograft tumor volume and body mass of nude mice were measured. Immunohistochemistry was used to detect the expression of PCNA , NF-B p65 and ZBTB7A. TUNEL and DAPI staining were used to detect the apoptosis of tumor cells.
Results: Curcumol combined with paclitaxel exerted a significant inhibitory effect on proliferation of MDA-MB-231 cells in the CCK-8 laboratory test. Hoechst 33258 immunofluorescence staining, flow cytometry, TUNEL, and DAPI apoptosis staining demonstrated that cell apoptosis was the highest in the CC+PTX group in vivo and in vitro. Expression of PCNA, Bcl-2, ZBTB7A, p-p65, and NF-B p65 was lowest in the CC+PTX group, while the expression of Bax was highest. The growth of xenograft tumors in the CC+PTX group was most notably suppressed. Immunohistochemistry showed that expression of PCNA, ZBTB7A, and NF-ƙB p65 was the lowest in the CC+PTX group.
Conclusion: Curcumol combined with paclitaxel exerted a synergistic antiproliferative and proapoptotic effect on triple-negative breast cancer cells.
کلیدواژه‌ها
Curcumol؛ NF-ƙB؛ Paclitaxel؛ TNBC؛ ZBTB7A
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