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Akbarin, Mohammad, Rafatpanah, Houshang, Soleimanpour, Saman, Amini, Abbas, Arian, Amirali, Mosavat, Arman, Rezaee, Seyed Abdolrahim. (1401). TAX and HBZ: hFc Ɣ 1 proteins as targets for passive immunotherapy. , 25(5), 586-596. doi: 10.22038/ijbms.2022.64787.14266
Mohammad Mehdi Akbarin; Houshang Rafatpanah; Saman Soleimanpour; Abbas Ali Amini; Amirali Arian; Arman Mosavat; Seyed Abdolrahim Rezaee. "TAX and HBZ: hFc Ɣ 1 proteins as targets for passive immunotherapy". , 25, 5, 1401, 586-596. doi: 10.22038/ijbms.2022.64787.14266
Akbarin, Mohammad, Rafatpanah, Houshang, Soleimanpour, Saman, Amini, Abbas, Arian, Amirali, Mosavat, Arman, Rezaee, Seyed Abdolrahim. (1401). 'TAX and HBZ: hFc Ɣ 1 proteins as targets for passive immunotherapy', , 25(5), pp. 586-596. doi: 10.22038/ijbms.2022.64787.14266
Akbarin, Mohammad, Rafatpanah, Houshang, Soleimanpour, Saman, Amini, Abbas, Arian, Amirali, Mosavat, Arman, Rezaee, Seyed Abdolrahim. TAX and HBZ: hFc Ɣ 1 proteins as targets for passive immunotherapy. , 1401; 25(5): 586-596. doi: 10.22038/ijbms.2022.64787.14266

TAX and HBZ: hFc Ɣ 1 proteins as targets for passive immunotherapy

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 25، شماره 5، مرداد 2022، صفحه 586-596    اصل مقاله (1.38 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.64787.14266
نویسندگان
Mohammad Mehdi Akbarin1؛ Houshang Rafatpanah1؛ Saman Soleimanpour2؛ Abbas Ali Amini3؛ Amirali Arian4؛ Arman Mosavat5؛ Seyed Abdolrahim Rezaee* 1
1Inflammation and Inflammatory Diseases Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3Cancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
4Animal Laboratory, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5Blood Borne Infections Research Center, Academic Center for Education, Culture, and Research (ACECR), Razavi Khorasan, Mashhad, Iran
چکیده
Objective(s): Human T leukemia virus type one (HTLV-1) causes two life-threatening diseases in around five percent of infected subjects, a T cell malignancy and a neurodegenerative disease. TAX and HBZ are the main virulence agents implicated in the manifestation of HTLV-1–associated diseases. Therefore, this study aims to produce these HTLV-1 factors as recombinant Fc fusion proteins to study the structures, their immunogenic properties as vaccines, and their capability to produce specific neutralization antibodies.
Materials and Methods: TAX and HBZ sequences were chosen from the NCBI-nucleotide database, then designed as human Fc chimers and cloned into Pichia pastoris. Produced proteins were purified by HiTrap affinity chromatography and subcutaneously injected into rabbits. Rabbit Abs were purified by batch chromatography, and their neutralization activities for the HTLV-1-infected MT-2 cell line were assessed. Furthermore, the protective abilities of recombinant proteins were evaluated in Tax or HBZ immunized rabbits by MT-2 cell line inoculation and measurement of HTLV-1-proviral load.
Results: Specific Abs against Tax and HBZ can eliminate 2 million MT-2 cells in 1/1000 dilution in vitro. In challenging assays, the immunization of the animals using Tax or HBZ had no protective activity as HTLV-1 PVL was still positive.
Conclusion: The result suggests that recombinant TAX and HBZ: hFcγ1 proteins can produce a proper humoral immune response. Therefore, they could be considered a passive immunotherapy source for HTLV-1-associated diseases, while total TAX and HBZ proteins are unsuitable as HTLV-1 vaccine candidates. 
کلیدواژه‌ها
ATLL؛ HBZ؛ HTLV؛ Immunization passive؛ Pichia pastoris؛ Recombinant proteins؛ TAX
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