Cetin, Nihal, Dasdelen, Dervis, Mogulkoc, Rasim, Menevse, Esma, Baltaci, Abdulkerim. (1401). Role of exogenous putrescine in the status of energy, DNA damage, inflammation, and spermidine/spermine-n(1)- acetyltransferase in brain ischemia-reperfusion in rats. , 25(5), 597-603. doi: 10.22038/ijbms.2022.63733.14046
Nihal Cetin; Dervis Dasdelen; Rasim Mogulkoc; Esma Menevse; Abdulkerim Kasim Baltaci. "Role of exogenous putrescine in the status of energy, DNA damage, inflammation, and spermidine/spermine-n(1)- acetyltransferase in brain ischemia-reperfusion in rats". , 25, 5, 1401, 597-603. doi: 10.22038/ijbms.2022.63733.14046
Cetin, Nihal, Dasdelen, Dervis, Mogulkoc, Rasim, Menevse, Esma, Baltaci, Abdulkerim. (1401). 'Role of exogenous putrescine in the status of energy, DNA damage, inflammation, and spermidine/spermine-n(1)- acetyltransferase in brain ischemia-reperfusion in rats', , 25(5), pp. 597-603. doi: 10.22038/ijbms.2022.63733.14046
Cetin, Nihal, Dasdelen, Dervis, Mogulkoc, Rasim, Menevse, Esma, Baltaci, Abdulkerim. Role of exogenous putrescine in the status of energy, DNA damage, inflammation, and spermidine/spermine-n(1)- acetyltransferase in brain ischemia-reperfusion in rats. , 1401; 25(5): 597-603. doi: 10.22038/ijbms.2022.63733.14046

Role of exogenous putrescine in the status of energy, DNA damage, inflammation, and spermidine/spermine-n(1)- acetyltransferase in brain ischemia-reperfusion in rats

Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 25، شماره 5، مرداد 2022، صفحه 597-603    اصل مقاله (547.6 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.63733.14046
نویسندگان
Nihal Cetin* 1؛ Dervis Dasdelen2؛ Rasim Mogulkoc2؛ Esma Menevse3؛ Abdulkerim Kasim Baltaci2
1Department of Pharmacology, Faculty of Medicine, Selcuk University, Konya, Turkey
2Department of Physiology, Faculty of Medicine, Selcuk University, Konya, Turkey
3Department of Biochemistry, Faculty of Medicine, Selcuk University, Konya, Turkey
چکیده
Objective(s): This study aims to investigate the role of putrescine against brain ischemia-reperfusion (IR) injured rats administered with 250 µmol/kg exogenous putrescine and highlight the IR-associated mechanisms in energy metabolism and inflammatory pathway. 
Materials and Methods: The rats were divided into six groups: 1-Sham group; 2-IR group, 30 min of ischemia and 30 min of reperfusion was performed with bilateral carotid occlusion (BCAO); 3-IPR group, a single oral dose of putrescine was administered at the start of the 30-minute reperfusion; while in the other treatment groups, 4 doses of putrescine were given within 12-hour intervals. After 30 min of reperfusion, the first dose was administered immediately in the IR-PI (group 4), after 3 hr in IR-PII (group 5), and after 6 hr in IR-PIII (group 6). Interleukin-6 (IL-6), Nuclear factor NF-kappa-B (NF-kB), Adenosine triphosphate (ATP), total Nitric oxide (NO), 8-hydroxyguanosine (8-OHdG), Spermidine/Spermin N-acetyltransferase (SSAT) levels were analyzed in brain tissues.
Results: IR reduced brain ATP levels; however, putrescine treatment reversed this state. Brain NO and 8-OHdG levels, and NF-kB and IL-6 levels increased significantly in the IR group and these elevations were decreased in putrescine administered groups. SSAT levels were higher in the IR-PII group. The lowest levels were observed in the IR-PIII group. 
Conclusion: The exogenous putrescine supplementation after cerebral IR creates neuroprotective effects independent of the time of administration; according to conditions such as formation of radicals in the brain, the spread of the inflammation and the need for consumption of energy are considered as a whole. 
کلیدواژه‌ها
8-OHdG؛ Brain ischemia؛ IL-6؛ Nitric oxide؛ NF-kB؛ Putrescine؛ Rat؛ Spermidine/Spermine-N(1)
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