سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Etemad, Leila, Montazeri, Danial, Moshiri, Mahammad, Jaafari, Mahmoud Reza, Arabzadeh, Sepideh, Karimi, Gholamreza. (1401). Protective effects of nanomicelle curcumin on the phosphine-induced oxidative stress and apoptosis in human liver HepG2 cells. , 9(2), 156-163. doi: 10.22038/nmj.2022.63370.1662
Leila Etemad; Danial Montazeri; Mahammad Moshiri; Mahmoud Reza Jaafari; Sepideh Arabzadeh; Gholamreza Karimi. "Protective effects of nanomicelle curcumin on the phosphine-induced oxidative stress and apoptosis in human liver HepG2 cells". , 9, 2, 1401, 156-163. doi: 10.22038/nmj.2022.63370.1662
Etemad, Leila, Montazeri, Danial, Moshiri, Mahammad, Jaafari, Mahmoud Reza, Arabzadeh, Sepideh, Karimi, Gholamreza. (1401). 'Protective effects of nanomicelle curcumin on the phosphine-induced oxidative stress and apoptosis in human liver HepG2 cells', , 9(2), pp. 156-163. doi: 10.22038/nmj.2022.63370.1662
Etemad, Leila, Montazeri, Danial, Moshiri, Mahammad, Jaafari, Mahmoud Reza, Arabzadeh, Sepideh, Karimi, Gholamreza. Protective effects of nanomicelle curcumin on the phosphine-induced oxidative stress and apoptosis in human liver HepG2 cells. , 1401; 9(2): 156-163. doi: 10.22038/nmj.2022.63370.1662

Protective effects of nanomicelle curcumin on the phosphine-induced oxidative stress and apoptosis in human liver HepG2 cells

Nanomedicine Journal
مقاله 6، دوره 9، شماره 2، تیر 2022، صفحه 156-163    اصل مقاله (858.37 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2022.63370.1662
نویسندگان
Leila Etemad1، 2؛ Danial Montazeri3؛ Mahammad Moshiri4، 5؛ Mahmoud Reza Jaafari6؛ Sepideh Arabzadeh7؛ Gholamreza Karimi* 1، 3
1Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
2Department of Drug Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
4Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
5Department of Clinical Toxicology, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
6Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
7Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Aluminium phosphide is used worldwide as a rodenticide and fumigant against sorted grain. Accidental or intentional exposure to aluminium phosphide (determined as PH3) results in extreme toxic effects on human. Due to the lack of a specific antidote, management of PH3 poisoning remains supportive and symptomatic. Curcumin, the main compounds of turmeric, has been reported to possess strong antioxidant and anti-apoptotic properties. This study was conducted to evaluate the effect of nanocurcumin on PH3 toxicity in HepG2 cell line. 
Materials and Methods: For this study, the cells pretreated with nanocurcumin (nCUR) and then exposed to PH3 for 6 hr. The cytotoxic and apoptotic effects of PH3 and nCUR were evaluated by MTT and propidium iodide flow cytometry. Indeed, the level of reactive oxygen species and glutathione were determined by fluorometric and colorimetric method. The oxidative DNA damage (8-OHdG) marker was also measured by ELISA kit. 
Results: Pretreatment with nCUR elevated the cell viability in PH3-treated cells and antagonized the PH3-induced glutathione depletion at high doses. Indeed, a significant decrease in the level of ROS and 8-OHdG as well as apoptotic activities were observed following exposure to nCUR.
Conclusion: These results indicated that nCUR could protect HepG2 cells against PH3 induced cell injury by attenuation of ROS and increasing GSH level. The nCUR efficiently suppressed increased apoptosis activity and formation of 8-OHdG and ultimately improved cell viability. Therefore, nCUR can be considered as promising therapeutic agents in treatment of aluminium phosphide poisoning.
کلیدواژه‌ها
Aluminium phosphide؛ Curcumin؛ Nanomicelle؛ Phosphin؛ Toxicity
مراجع
1.    Soltani M, Shetab-Boushehri SF, Mohammadi H, Shetab-Boushehri SV. Proposing boric acid as an antidote for aluminium phosphide poisoning by investigation of the chemical reaction between boric acid and phosphine. JMHI. 2013;7:21-24.
2.    Kariman H, Heydari K, Fakhri M, Shahrami A, Dolatabadi AA, Mohammadi HA, et al. Aluminium phosphide poisoning and oxidative stress: serum biomarker assessment. J Med Toxicol. 2012;8(3):281-284.
3.    Pokhrel D, Pant S, Pradhan A, Mansoor S. A comparative retrospective study of poisoning cases in central, zone and district hospitals. Kathmandu Univ J Sci Eng Tech. 2008;1:40-48.
4.    Proudfoot AT. Aluminium and zinc phosphide poisoning. Clin Toxicol 2009;47(2):89-100.
5.    Rashedinia M, Jamshidzadeh A, Mehrabadi AR, Niknahad H. Prevention of phosphine-induced cytotoxicity by nutrients in HepG2 cells. Indian J Med Res. 2016;144(4):560-565.
6.    Hsu CH, Quistad GB, Casida JE. Phosphine-induced oxidative stress in Hepa 1c1c7 cells. Toxicological sciences: an official journal of the Society of Toxicology. 1998;46(1):204-210.
7.    Nath NS, Bhattacharya I, Tuck AG, Schlipalius DI, Ebert PR. Mechanisms of phosphine toxicity. J Toxicol. 2011;2011:494168.
8.    Moghadamnia AA. An update on toxicology of aluminum phosphide. Daru. 2012;20(1):25.
9.    Karimani A, Mohammadpour AH, Zirak MR, Rezaee R, Megarbane B, Tsatsakis A, et al. Antidotes for aluminum phosphide poisoning - An update. Toxicol Rep. 2018;5:1053-1059.
10.    Asghari G, Mostajeran A, Shebli M. Curcuminoid and essential oil components of turmeric at different stages of growth cultivated in Iran. Res Pharm Sci. 2009;4(1):55-61.
11.    Valokola MG, Karimi G, Razavi BM, Kianfar M, Jafarian AH, Jaafari MR, et al. The protective activity of nanomicelle curcumin in bisphenol A-induced cardiotoxicity following subacute exposure in rats. Environ Toxicol. 2019;34(3):319-329.
12.    Li S, Yuan W, Deng G, Wang P, Yang P, Aggarwa BB. Chemical composition and product quality control of Turmeric (Curcuma longa L.). Crops. 2011;2:28-54.
13.    Jurenka JS. Anti-inflammatory properties of curcumin, a major constituent of Curcuma longa: a review of preclinical and clinical research. Altern Med Rev. 2009;14(2):141-153.
14.    Duvoix A, Blasius R, Delhalle S, Schnekenburger M, Morceau F, Henry E, et al. Chemopreventive and therapeutic effects of curcumin. Cancer letters. 2005;223(2):181-190.
15.    Gupta SC, Patchva S, Aggarwal BB. Therapeutic roles of curcumin: lessons learned from clinical trials. AAPS J. 2013;15(1):195-218.
16.    Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB. Bioavailability of curcumin: problems and promises. Molecular pharmaceutics. 2007;4(6):807-818.
17.    Rahmani m, Golian A, Kermanshahi H, Bassami MR. Effects of curcumin or nanocurcumin on blood biochemical parameters, intestinal morphology and microbial population of broiler chickens reared under normal and cold stress conditions. J APPL ANIM RES. 2018;46(1):200-209.
18.    Ma Z, Shayeganpour A, Brocks DR, Lavasanifar A, Samuel J. High-performance liquid chromatography analysis of curcuminin rat plasma: application to pharmacokinetics of polymericmicellarformulation of curcumin. Biomed Chromatogr. 2007;21:546-552.
19.    Hatamipour M, Sahebkar A, Alavizadeh SH, Dorri M, Jaafari MR. Novel nanomicelle formulation to enhance bioavailability and stability of curcuminoids. Iran J Basic Med Sci. 2019;22(3):282-289.
20.    Moallem SA, Imenshahidi M, Shahini N, Javan AR, Karimi M, Alibolandi M, et al. Synthesis, anti-inflammatory and anti-nociceptive activities and cytotoxic effect of novel thiazolidin-4-ones derivatives as selective cyclooxygenase (COX-2) inhibitors. Iran J Basic Med Sci. 2013;16(12):1238.
21.    Rashidi R, Moallem SA, Moshiri M, Hadizadeh F, Etemad L. Protective Effect of Cinnamaldehyde on METH-induced Neurotoxicity in PC12 Cells via Inhibition of Apoptotic Response and Oxidative Stress. Iran J Pharm Res. 2021;20(2):135-143.
22.    Mansouri m, Moallem SA, Asili J, Etemad L. Cytotoxic and Apoptotic Effects of Scrophularia Umbrosa Dumort Extract on MCF-7 Breast Cancer and 3T3 Cells. RBMB. 2019;8(1).
23.    Khorashadizadeh N, Neamati A, Moshiri M, Etemad L. Verbascoside inhibits paraquate-induced pulmonary toxicity via modulating oxidative stress, inflammation, apoptosis and DNA damage in A549 cell. Drug Chem Toxicol. 2021:1-9.
24.    Hosseinzadeh L, Malekshahi A, Ahmadi F, Emami SA, Hajialyani M, Mojarrab M. The Protective Effect of Different Extracts of Three Artemisia Species against H2O2-Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells. Pharmacognosy Res. 2018;10(1):64-71.
25.    Ahmadimanesh M, Abbaszadegan MR, Morshedi Rad D, Moallem SA, Mohammadpour AH, Ghahremani MH, et al. Effects of selective serotonin reuptake inhibitors on DNA damage in patients with depression. J Psychopharmacol. 2019;33(11):1364-1376.
26.    Anand R, Kumari P, Kaushal A, Bal A, Wani WY, Sunkaria A, et al. Effect of acute aluminum phosphide exposure on rats: a biochemical and histological correlation. Toxicol Lett. 2012;215(1):62-69.
27.    Asghari MH, Abdollahi M, de Oliveira MR, Nabavi SM. A review of the protective role of melatonin during phosphine-induced cardiotoxicity: focus on mitochondrial dysfunction, oxidative stress and apoptosis. J Pharm Pharmacol. 2017;69(3):236-243.
28.    Dinkova-Kostova AT, Talalay P. Direct and indirect antioxidant properties of inducers of cytoprotective proteins. Molecular nutrition & food research. 2008;52 Suppl 1:S128-S138.
29.    Trujillo J, Chirino YI, Molina-Jijon E, Anderica-Romero AC, Tapia E, Pedraza-Chaverri J. Renoprotective effect of the antioxidant curcumin: Recent findings. Redox biology. 2013;1:448-456.
30.    Barclay LR, Vinqvist MR, Mukai K, Goto H, Hashimoto Y, Tokunaga A, et al. On the antioxidant mechanism of curcumin: classical methods are needed to determine antioxidant mechanism and activity. Org Lett. 2000;2(18):2841-2843.
31.    Menon VP, Sudheer AR. Antioxidant and anti-inflammatory properties of curcumin. Adv Exp Med Biol. 2007;595:105-125.
32.    Hewlings SJ, Kalman DS. Curcumin: A Review of Its’ Effects on Human Healt. Foods. 2017;6:1-11.
33.    Ayobola A. Assessment of lipid peroxidation and activities of antioxidant enzymes in phosphide‐powder residue exposed rats. J Drug Metab Toxicol 2012;3:1-4.
34.    Loganes C, Lega S, Bramuzzo M, Vecchi Brumatti L, Piscianz E, Valencic E, et al. Curcumin Anti-Apoptotic Action in a Model of Intestinal Epithelial Inflammatory Damage. Nutrients. 2017;9(6).
35.    Park SI, Lee EH, Kim SR, Jang YP. Anti-apoptotic effects of Curcuma longa L. extract and its curcuminoids against blue light-induced cytotoxicity in A2E-laden human retinal pigment epithelial cells. The Journal of Pharmacy and Pharmacology. 2017;69(3):334-340.
36.    Topcu-Tarladacalisir Y, Akpolat M, Uz YH, Kizilay G, Sapmaz-Metin M, Cerkezkayabekir A, et al. Effects of curcumin on apoptosis and oxidoinflammatory regulation in a rat model of acetic acid-induced colitis: the roles of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase. J Med Food. 2013;16(4):296-305.
37.    Lee SC, Jee SC, Kim M, Kim S, Shin MK, Kim Y, et al. Curcumin Suppresses the Lipid Accumulation and Oxidative Stress Induced by Benzo[a]pyrene Toxicity in HepG2 Cells. Antioxidants. 2021;10(8).
38.    Syng-Ai C, Kumari AL, Khar A. Effect of curcumin on normal and tumor cells: role of glutathione and bcl-2. Molecular cancer therapeutics. 2004;3(9):1101-1108.
39.    Wang JL, Wang JJ, Cai ZN, Xu CJ. The effect of curcumin on the differentiation, apoptosis and cell cycle of neural stem cells is mediated through inhibiting autophagy by the modulation of Atg7 and p62. Int J Mol Med. 2018;42(5):2481-2488.
40.    Cao J, Liu Y, Jia L, Jiang LP, Geng CY, Yao XF, et al. Curcumin attenuates acrylamide-induced cytotoxicity and genotoxicity in HepG2 cells by ROS scavenging. J Agric Food Chem. 2008;56(24):12059-12063.
41.    Roy M, Sinha D, Mukherjee S, Biswas J. Curcumin prevents DNA damage and enhances the repair potential in a chronically arsenic-exposed human population in West Bengal, India. Eur J Cancer Prev. 2011;20(2):123-131.
42.    Hosseinzadehdehkordi M, Adelinik A, Tashakor A. Dual effect of curcumin targets reactive oxygen species, adenosine triphosphate contents and intermediate steps of mitochondria-mediated apoptosis in lung cancer cell lines. Eur J Pharmacol. 2015;769:203-210.
43.    Chen Q, Wang Y, Xu K, Lu G, Ying Z, Wu L, et al. Curcumin induces apoptosis in human lung adenocarcinoma A549 cells through a reactive oxygen species-dependent mitochondrial signaling pathway. Oncol Rep. 2010;23(2):397-403.
44.    Banerjee A, Kunwar A, Mishra B, Priyadarsini KI. Concentration dependent antioxidant/pro-oxidant activity of curcumin studies from AAPH induced hemolysis of RBCs. Chemico-biological interactions. 2008;174(2):134-139.
45.    Liu W, Zhai Y, Heng X, Che FY, Chen W, Sun D, et al. Oral bioavailability of curcumin: problems and advancements. J Drug Target. 2016;24(8):694-702.
46.    Rahimi HR, Mohammadpour AH, Dastani M, Jaafari MR, Abnous K, Ghayour Mobarhan M, et al. The effect of nano-curcumin on HbA1c, fasting blood glucose, and lipid profile in diabetic subjects: a randomized clinical trial. Avicenna J Phytomed. 2016;6(5):567-577.

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