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Razi, Omid, Parnow, Abdolhossein, Rashidi, Iraj, Pakravan, Nafiseh, Nedaei, Seyed Ershad, Motl, Robert. (1400). Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis. , 25(2), 245-253. doi: 10.22038/ijbms.2022.61671.13645
Omid Razi; Abdolhossein Parnow; Iraj Rashidi; Nafiseh Pakravan; Seyed Ershad Nedaei; Robert Motl. "Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis". , 25, 2, 1400, 245-253. doi: 10.22038/ijbms.2022.61671.13645
Razi, Omid, Parnow, Abdolhossein, Rashidi, Iraj, Pakravan, Nafiseh, Nedaei, Seyed Ershad, Motl, Robert. (1400). 'Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis', , 25(2), pp. 245-253. doi: 10.22038/ijbms.2022.61671.13645
Razi, Omid, Parnow, Abdolhossein, Rashidi, Iraj, Pakravan, Nafiseh, Nedaei, Seyed Ershad, Motl, Robert. Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis. , 1400; 25(2): 245-253. doi: 10.22038/ijbms.2022.61671.13645

Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis

Iranian Journal of Basic Medical Sciences
مقاله 15، دوره 25، شماره 2، اردیبهشت 2022، صفحه 245-253    اصل مقاله (541.33 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.61671.13645
نویسندگان
Omid Razi1؛ Abdolhossein Parnow* 1؛ Iraj Rashidi2؛ Nafiseh Pakravan3؛ Seyed Ershad Nedaei4؛ Robert Motl5
1Department of Exercise Physiology, Faculty of Physical Education and Sports Science, Razi University, Kermanshah, Iran
2Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
3Department of Immunology, School of Medicine, Alborz University of Medical Science, Karaj, Iran
4Department of Physiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
5Department of Kinesiology and Nutrition, College of Applied Health Sciences, University of Illinois Chicago, United States of America
چکیده
Objective(s): Blood-brain barrier (BBB) permeability is central in multiple sclerosis (MS) pathophysiology, and exercise may improve BBB integrity. The current study investigated the prophylactic and/ or therapeutic role of aerobic exercise (EX) training on BBB integrity in experimental autoimmune encephalomyelitis (EAE). 
Materials and Methods: Forty female Lewis rats were randomly divided into four groups. The experimental groups included: no-EAE induction+ no-exercise (no-EAE+ no-EX), no-EAE induction+ exercise (no-EAE+EX), EAE induction+ no-exercise (EAE+ no-EX), and EAE induction+ exercise (EAE+EX). The no-EAE+EX and EAE+EX groups performed six weeks of progressive aerobic exercise training. GFAP, angiopoietin 1 (Ang-1) expression, tight-junction (TJ) proteins of claudin-5 and occludin were measured as components of BBB integrity and the rate of neuronal apoptosis was evaluated in hippocampi. 
Results: A significant increase in GFAP and Ang-1 expression (P<0.001) and conversely a down-regulation in TJ proteins (P<0.05) was found in the brains of the no-EAE+EX group compared with the no-EAE+ no-EX group. The expression of GFAP and Ang-1 proteins significantly increased in the hippocampi of the EAE+ no-EX group (P<0.001), whereas aerobic training (in the EAE+EX group) meaningfully reversed such increases (P<0.001). Besides, down-regulated TJ proteins and increased neuronal apoptosis induced by EAE induction (EAE+ no-EX group) were restored and reduced, respectively, by aerobic training in the CNS of the EAE+EX group (P<0.001). 
Conclusion: The provision of a six-week treadmill aerobic training buffered the detrimental effects of EAE on BBB integrity and consequently neuronal apoptosis. 
کلیدواژه‌ها
Aerobic training؛ Astrogliosis؛ Brain barrier dysfunction؛ Claudin-5؛ EAE model؛ Occludin
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