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Firouzi, Zohreh, Jaafari, Mahmoud, Sankian, Mojtaba, Zare, Sirwan, Tafaghodi, Mohsen. (1400). A novel nanomicelle composed from PEGylated TB di-peptide could be successfully used as a BCG booster. , 25(2), 223-231. doi: 10.22038/ijbms.2022.61373.13583
Zohreh Firouzi; Mahmoud Reza Jaafari; Mojtaba Sankian; Sirwan Zare; Mohsen Tafaghodi. "A novel nanomicelle composed from PEGylated TB di-peptide could be successfully used as a BCG booster". , 25, 2, 1400, 223-231. doi: 10.22038/ijbms.2022.61373.13583
Firouzi, Zohreh, Jaafari, Mahmoud, Sankian, Mojtaba, Zare, Sirwan, Tafaghodi, Mohsen. (1400). 'A novel nanomicelle composed from PEGylated TB di-peptide could be successfully used as a BCG booster', , 25(2), pp. 223-231. doi: 10.22038/ijbms.2022.61373.13583
Firouzi, Zohreh, Jaafari, Mahmoud, Sankian, Mojtaba, Zare, Sirwan, Tafaghodi, Mohsen. A novel nanomicelle composed from PEGylated TB di-peptide could be successfully used as a BCG booster. , 1400; 25(2): 223-231. doi: 10.22038/ijbms.2022.61373.13583

A novel nanomicelle composed from PEGylated TB di-peptide could be successfully used as a BCG booster

Iranian Journal of Basic Medical Sciences
مقاله 12، دوره 25، شماره 2، اردیبهشت 2022، صفحه 223-231    اصل مقاله (648.94 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.61373.13583
نویسندگان
Zohreh Firouzi1، 2، 3؛ Mahmoud Reza Jaafari1، 3؛ Mojtaba Sankian4؛ Sirwan Zare4؛ Mohsen Tafaghodi* 1، 3
1Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
3Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
4Immunobiochemistry Laboratory, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Tuberculosis affects one-third of the world’s population and leads to a high rate of morbidity and mortality. Bacillus Chalmette–Guerin (BCG) as the only approved vaccine for the Mycobacterium tuberculosis (Mtb) does not show enough protection in the vaccinated population. 
Materials and Methods: The main aim of this study was to prepare a self-assembled nanomicelle composed from a di-block polymer in which, a di-fusion peptide was the hydrophobic block and polyethylene glycol (PEG) was the hydrophilic block. The micelles were characterized in vitro and in vivo as an antigen delivery system/adjuvant both with and without a prime BCG. 
Results: The micellar nanovaccine was able to elicit good dendritic cell maturation. Nanomicelles could efficiently induce systemic cytokines as well as nasal secretory predominant antibody titers (sIgA). The expression pattern of cytokines indicated the superiority of cellular immunity. Nasal administration of two doses of nanomicelles after a prime subcutaneous administration of BCG induced the highest mucosal and systemic immune responses. 
Conclusion: Based on our results PEG-HspX/EsxS self-assembled nanomicelle is highly immunogenic and can be considered a potential vaccine candidate against Mtb to boost BCG efficiency.
کلیدواژه‌ها
Nanovaccine؛ Nasal and parenteral immunization؛ Recombinant fusion peptide؛ Self-assembled nanomicelles؛ Tuberculosis
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