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Hakimizadeh, Elham, Kaeidi, Ayat, Rahmani, Mohammad reza, Allahtavakoli, Mohammad, Hassanshahi, Jalal. (1400). Calcium dobesilate ameliorates hepatorenal injury induced by carbon tetrachloride in mice. , 25(2), 148-154. doi: 10.22038/ijbms.2022.61499.13606
Elham Hakimizadeh; Ayat Kaeidi; Mohammad reza Rahmani; Mohammad Allahtavakoli; Jalal Hassanshahi. "Calcium dobesilate ameliorates hepatorenal injury induced by carbon tetrachloride in mice". , 25, 2, 1400, 148-154. doi: 10.22038/ijbms.2022.61499.13606
Hakimizadeh, Elham, Kaeidi, Ayat, Rahmani, Mohammad reza, Allahtavakoli, Mohammad, Hassanshahi, Jalal. (1400). 'Calcium dobesilate ameliorates hepatorenal injury induced by carbon tetrachloride in mice', , 25(2), pp. 148-154. doi: 10.22038/ijbms.2022.61499.13606
Hakimizadeh, Elham, Kaeidi, Ayat, Rahmani, Mohammad reza, Allahtavakoli, Mohammad, Hassanshahi, Jalal. Calcium dobesilate ameliorates hepatorenal injury induced by carbon tetrachloride in mice. , 1400; 25(2): 148-154. doi: 10.22038/ijbms.2022.61499.13606

Calcium dobesilate ameliorates hepatorenal injury induced by carbon tetrachloride in mice

Iranian Journal of Basic Medical Sciences
مقاله 2، دوره 25، شماره 2، اردیبهشت 2022، صفحه 148-154    اصل مقاله (602.09 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.61499.13606
نویسندگان
Elham Hakimizadeh1؛ Ayat Kaeidi2؛ Mohammad reza Rahmani3، 2؛ Mohammad Allahtavakoli2؛ Jalal Hassanshahi* 2
1Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan,Iran
2Department of Physiology and Pharmacology, School of medicine. Rafsanjan University of Medical Sciences, Rafsanjan, Iran
3Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
چکیده
Objective(s): Calcium dobesilate (CaD) has anti-oxidant, anti-inflammatory, and anti-apoptotic effects. In this study, the protective effects of CaD against hepatorenal damage induced by carbon tetrachloride (CCl4) in mice were evaluated. 
Materials and Methods: Thirty male mice were randomly divided into five groups: Control, CaD 100 mg/kg, CCl4, CCl4+CaD 50 mg/kg, and CCl4+CaD 100 mg/kg. CaD (50 and 100 mg/kg) was administered orally once a day for 4 weeks. The liver and kidney indices (serum creatinine, blood urine nitrogen, alanine aminotransferase, and aspartate aminotransferase levels) were determined. Also, liver and kidney tissue oxidant/anti-oxidant markers (glutathione peroxidase, malondialdehyde, total anti-oxidant capacity, and superoxide dismutase) were measured. Cleaved caspase-3, Bax, cytochrome-c, and Bcl-2 protein levels were measured by immunoblotting method in the liver and kidney tissues. The liver and kidney histopathological changes were evaluated by the Hematoxylin and Eosin (H&E) staining method.
Results: CCl4 induced significant oxidative stress and apoptosis in kidney and liver tissues that was concomitant with histopathological abnormalities in these organs in the CCl4 group versus the control (P<0.05). However, CaD (100 mg/kg) could significantly improve the histopathological change in the liver and kidney tissues of CCl4+CaD 100 mg/kg mice versus the CCl4 group (P<0.05). In addition, CaD (100 mg/kg) attenuated the pro and anti-apoptotic markers in the liver and kidney tissues of CCl4+CaD 100 mg/kg mice versus the CCl4 group (P<0.05).
Conclusion: CaD (100 mg/kg) has a protective effect against hepatorenal injury induced by CCl4 at least via its anti-apoptotic and anti-oxidant properties.
کلیدواژه‌ها
Apoptosis؛ Calcium dobesilate؛ Carbon tetrachloride؛ Mice؛ Oxidative stress
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