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Radmehr, Vahid, Ahangarpour, Akram, Mard, Seyyed Ali, Khorsandi, Layasadat. (1400). Crocin ameliorates MicroRNAs-associated ER stress in type 2 diabetes induced by methylglyoxal. , 25(2), 179-186. doi: 10.22038/ijbms.2022.60493.13407
Vahid Radmehr; Akram Ahangarpour; Seyyed Ali Mard; Layasadat Khorsandi. "Crocin ameliorates MicroRNAs-associated ER stress in type 2 diabetes induced by methylglyoxal". , 25, 2, 1400, 179-186. doi: 10.22038/ijbms.2022.60493.13407
Radmehr, Vahid, Ahangarpour, Akram, Mard, Seyyed Ali, Khorsandi, Layasadat. (1400). 'Crocin ameliorates MicroRNAs-associated ER stress in type 2 diabetes induced by methylglyoxal', , 25(2), pp. 179-186. doi: 10.22038/ijbms.2022.60493.13407
Radmehr, Vahid, Ahangarpour, Akram, Mard, Seyyed Ali, Khorsandi, Layasadat. Crocin ameliorates MicroRNAs-associated ER stress in type 2 diabetes induced by methylglyoxal. , 1400; 25(2): 179-186. doi: 10.22038/ijbms.2022.60493.13407

Crocin ameliorates MicroRNAs-associated ER stress in type 2 diabetes induced by methylglyoxal

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 25، شماره 2، اردیبهشت 2022، صفحه 179-186    اصل مقاله (1.13 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.60493.13407
نویسندگان
Vahid Radmehr1؛ Akram Ahangarpour* 2؛ Seyyed Ali Mard3، 4؛ Layasadat Khorsandi5
1Student Research Committee, Department of Physiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2Medical Basic Sciences Research Institute, Physiology Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3Medical Basic Sciences Research Institute, Physiology Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4Alimentary tract research center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
5Department of Anatomical Sciences, School of Medicine, Medical Basic Sciences Research Institute, Cellular and molecular research center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
Objective(s): Methylglyoxal (MG) provokes endoplasmic reticulum (ER) stress in β-cells and triggers pancreatic β-cell dysfunction. Crocin has anti-diabetic properties. The present study investigated whether crocin prevented pancreas damages induced by MG.
Materials and Methods: Diabetes was induced by MG administration (600 mg/kg/day, PO). On the fourteenth day, after proving hyperglycemia, crocin (15, 30, and 60 mg/kg) and metformin (MT) (150 mg/kg) were used for detoxification of MG until the end of the experiment. The animals were divided into 6 groups: 1) control, 2) diabetic by MG, 3) MG + crocin 15 mg/kg, 4) MG + crocin 30 mg/kg, 5) MG + crocin 60 mg/kg, and 6) MG + MT. The data were analyzed by one-way analysis of variance and significant differences were compared by Tukey and Bonferroni tests (P<0.05). Biochemical assays, antioxidant evaluation, and microRNAs expression associated with ER stress were assessed.
Results: MG induced hyperglycemia, insulin resistance, and dyslipidemia (P<0.001). Crocin and MT significantly ameliorated β-cell function through reduction of fasting blood glucose, malondialdehyde levels (P<0.001), and  significant elevation of anti-oxidant enzyme activity accompanied by regulation of glutathione and glyoxalase1-Nrf2 in MG induced diabetic mice. Crocin and MT significantly down-regulated microRNAs 204, 216b, 192, and 29a expression (P<0.001). Crocin (60 mg/kg) (P<0.01) and MT (P<0.001) could improve diameter of pancreatic islets in MG treated mice. 
Conclusion: Crocin prevents the progression of diabetes through modulating ER stress-associated microRNAs and GLO1 activity with the helpful effects of glutathione and Nrf2.
کلیدواژه‌ها
Crocin؛ Diabetes؛ ER stress؛ Glyoxalase 1؛ Methylglyoxal؛ MiR-204
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