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Zhang, Jichen, Zhao, Xuemei, Zhu, Hongling, Wang, Jingnan, Ma, Junhua, Gu, Mingjun. (1400). Crocin protects the renal tubular epithelial cells against high glucose-induced injury and oxidative stress via regulation of the SIRT1/Nrf2 pathway. , 25(2), 193-197. doi: 10.22038/ijbms.2022.51597.11708
Jichen Zhang; Xuemei Zhao; Hongling Zhu; Jingnan Wang; Junhua Ma; Mingjun Gu. "Crocin protects the renal tubular epithelial cells against high glucose-induced injury and oxidative stress via regulation of the SIRT1/Nrf2 pathway". , 25, 2, 1400, 193-197. doi: 10.22038/ijbms.2022.51597.11708
Zhang, Jichen, Zhao, Xuemei, Zhu, Hongling, Wang, Jingnan, Ma, Junhua, Gu, Mingjun. (1400). 'Crocin protects the renal tubular epithelial cells against high glucose-induced injury and oxidative stress via regulation of the SIRT1/Nrf2 pathway', , 25(2), pp. 193-197. doi: 10.22038/ijbms.2022.51597.11708
Zhang, Jichen, Zhao, Xuemei, Zhu, Hongling, Wang, Jingnan, Ma, Junhua, Gu, Mingjun. Crocin protects the renal tubular epithelial cells against high glucose-induced injury and oxidative stress via regulation of the SIRT1/Nrf2 pathway. , 1400; 25(2): 193-197. doi: 10.22038/ijbms.2022.51597.11708

Crocin protects the renal tubular epithelial cells against high glucose-induced injury and oxidative stress via regulation of the SIRT1/Nrf2 pathway

Iranian Journal of Basic Medical Sciences
مقاله 8، دوره 25، شماره 2، اردیبهشت 2022، صفحه 193-197    اصل مقاله (662.67 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.51597.11708
نویسندگان
Jichen Zhang1، 2؛ Xuemei Zhao1؛ Hongling Zhu1؛ Jingnan Wang1؛ Junhua Ma1؛ Mingjun Gu* 1
1Department of Endocrinology, Shanghai Pudong New District Gongli Hospital, Second Military Medical University, Shanghai 200135, P. R China
2Postgraduate Education College, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
چکیده
Objective(s): Renal tubular damage is critical pathological feathers of diabetic nephropathy (DN). This study aimed to explore the protective activity and related mechanisms of crocin in renal epithelial cell injury induced by high glucose.
Materials and Methods: Renal tubular epithelial HK-2 cells were cultured with D-glucose to establish an in vitro DN model. Cell viability was evaluated by CCK-8 assay. Apoptosis was detected by Annexin V-FITC kit. Oxidative stress was evaluated by colorimetry. RT-qPCR was carried out to determine the mRNA expressions of NF-E2-related factor 2 (Nrf2) and its pathway genes. Western blot was applied to determine the protein expressions of Nrf2 and related proteins.
Results: High glucose (5.5, 30, and 50 mM D-glucose) decreased cell viability at 72 hr, which was attenuated by crocin (25 and 50 μM). Crocin also attenuated the high glucose (30 mM D-glucose) induced apoptosis of HK-2 cells,  decreased MDA content, and increased SOD activity in culture media. Crocin increased mRNA levels of Nrf2, HO-1, and NQO1. Moreover, crocin increased protein expressions of Nrf2, Sirtuin 1 (SIRT1), and p-Akt (Ser473). Inhibition of Nrf2 using siRNA, and inhibitors of SIRT1 (nicotinamide, NAM, 20 μM) and PI3K/Akt (LY294002, 50 μM) all attenuated the protective effect of crocin. Nrf2 siRNA and NAM also partially attenuated the inhibitory effect on oxidative stress and increase in the Nrf2 protein by crocin treatment.
Conclusion: Crocin protects renal epithelial cells against injury induced by high glucose, and the mechanism is associated with partial activation of the SIRT1-Nrf2 pathway.
کلیدواژه‌ها
Diabetic nephropathy؛ High glucose؛ NF-E2-related factor 2؛ Oxidative stress؛ Renal tubular epithelial cell؛ Sirtuin 1
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