سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Duman, Yucel, Ersoy, Yasemin, Tanriverdi, Elif, Otlu, Baris, Altunisik Toplu, Sibel, Gozukara Bag, Harika, Tekerekoglu, Sait, Bulam, Nazire, Kaplan Canturk, Elif, Parmaksiz, Nalan. (1400). Oral colonization of Acinetobacter baumannii in intensive care units: Risk factors, incidence, molecular epidemiology, association with the occur of pneumonia and sepsis, and infection control measures. , 25(2), 239-244. doi: 10.22038/ijbms.2022.59713.13243
Yucel Duman; Yasemin Ersoy; Elif Seren Tanriverdi; Baris Otlu; Sibel Altunisik Toplu; Harika Gozde Gozukara Bag; Sait Mehmet Tekerekoglu; Nazire Bulam; Elif Kaplan Canturk; Nalan Parmaksiz. "Oral colonization of Acinetobacter baumannii in intensive care units: Risk factors, incidence, molecular epidemiology, association with the occur of pneumonia and sepsis, and infection control measures". , 25, 2, 1400, 239-244. doi: 10.22038/ijbms.2022.59713.13243
Duman, Yucel, Ersoy, Yasemin, Tanriverdi, Elif, Otlu, Baris, Altunisik Toplu, Sibel, Gozukara Bag, Harika, Tekerekoglu, Sait, Bulam, Nazire, Kaplan Canturk, Elif, Parmaksiz, Nalan. (1400). 'Oral colonization of Acinetobacter baumannii in intensive care units: Risk factors, incidence, molecular epidemiology, association with the occur of pneumonia and sepsis, and infection control measures', , 25(2), pp. 239-244. doi: 10.22038/ijbms.2022.59713.13243
Duman, Yucel, Ersoy, Yasemin, Tanriverdi, Elif, Otlu, Baris, Altunisik Toplu, Sibel, Gozukara Bag, Harika, Tekerekoglu, Sait, Bulam, Nazire, Kaplan Canturk, Elif, Parmaksiz, Nalan. Oral colonization of Acinetobacter baumannii in intensive care units: Risk factors, incidence, molecular epidemiology, association with the occur of pneumonia and sepsis, and infection control measures. , 1400; 25(2): 239-244. doi: 10.22038/ijbms.2022.59713.13243

Oral colonization of Acinetobacter baumannii in intensive care units: Risk factors, incidence, molecular epidemiology, association with the occur of pneumonia and sepsis, and infection control measures

Iranian Journal of Basic Medical Sciences
مقاله 14، دوره 25، شماره 2، اردیبهشت 2022، صفحه 239-244    اصل مقاله (453.85 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.59713.13243
نویسندگان
Yucel Duman* 1؛ Yasemin Ersoy2؛ Elif Seren Tanriverdi1؛ Baris Otlu1؛ Sibel Altunisik Toplu2؛ Harika Gozde Gozukara Bag3؛ Sait Mehmet Tekerekoglu1؛ Nazire Bulam4؛ Elif Kaplan Canturk4؛ Nalan Parmaksiz4
1Medical Microbiology Department. Inonu University Medical Faculty, Malatya, Turkey
2Infection Disease Department. Inonu University Medical Faculty, Malatya, Turkey
3Biostatistics and Medical Informatics Department. Inonu University Medical Faculty, Malatya, Turkey
4Infection Control Committee, Turgut Ozal Medical Center, Malatya, Turkey
چکیده
Objective(s): Oral colonization of Acinetobacter baumannii can lead to infections such as pneumonia and sepsis. We aimed to evaluate oral colonization of hospitalized patients in ICUs and to examine risk factors for oral colonization, molecular epidemiology, and incidence of pneumonia and sepsis. 
Materials and Methods: The study began in February 2021. Oral cultures were taken. The microorganisms were identified by a Maldi-tof MS mass spectrometry device. Colistin resistance genes were investigated by polymerase chain reaction. Clonal relationships were determined by pulsed-field gel electrophoresis. 
Results: A. baumannii was found in 21 of 96 patients’ oral cultures. Pneumonia and sepsis due to A. baumannii were detected in 14 and 5 patients, respectively. The mean growth time of A. baumannii from oral cultures was 11.8 days, and the meantime for the occurrence of pneumonia after oral growth was 5.2 days. We determined a plasmid mediated mcr-2 colistin resistance gene in a colistin susceptible A. baumannii strain. It is the first report of the plasmid mediated mcr-2 colistin resistance gene in our country. In total, fourteen different A. baumannii genotypes were determined in PFGE. It was determined that the effects of antibiotic use, oral motor dysfunction, mechanical ventilation, intubation, orogastric tube use, and total parenteral nutrition intake on oral colonization were statistically significant.
Conclusion: Oral colonization of A. baumannii is a significant concern in ICUs. We believe that it is important to take oral cultures and follow the risk factors and take infection control measures to prevent oral colonization of resistant isolates in ICUs.
کلیدواژه‌ها
Acinetobacter baumannii؛ Infection control measures؛ Intensive care units؛ mcr-2؛ Oral colonization
مراجع
1. Alp E, Coruh A, Gunay GK, Yontar Y, Doganay M. Risk factors for nosocomial infection and mortality in burn patients: 10 years of experience at a university hospital. J Burn Care Res 2012; 33:379e85.
2. Gao L, Xu T, Huang G, Jiang S, GuChen YF. Oral microbiomes: more and more importance in oral cavity and whole body. Protein Cell 2018; 9:488–500. 
3. Zaatout N. Presence of non-oral bacteria in the oral cavity. Arch Microbiol 2021; 31:1–14. 
4. Tacconelli E, Magrini N. Global priority list of antibiotic resistant bacteria to guide research, discovery and development of new antibiotics. World Health Organization 2017.
5. Duman Y, Tekerekoglu MS. Colistin MICs and resistance genes of Acinetobacter baumannii isolated in intensive care units. Turk J Intensive Care 2021; 19:71-75. 
6. Duman Y, Ersoy Y, Gursoy NC, Altunisik Toplu S, Otlu B. A silent outbreak due to Klebsiella pneumoniae that co-produced NDM-1 and OXA-48 carbapenemases, and infection control measures. Iran J Basic Med Sci 2020; 23:46-50.
7. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 2016; 16: 161-168.
8. Xavier BB, Lammens C, Ruhal R, Kumar-Singh S, Butaye P, Goossens H, et al. Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016. Euro Surveill 2016; 21: 30280.
9. Nasr P. Genetics, epidemiology, and clinical manifestations of multidrug-resistant Acinetobacter baumannii. J Hosp Infect 2020; 104:4-11. 
10. Karageorgopoulos DE, Falagas ME. Current control and treatment of multidrug-resistant Acinetobacter baumannii infections. Lancet 2008; 8: 751-762
11. Duman Y, Kuzucu C, Ersoy Y, Otlu B. The Effect of sodium dichloroisocyanurate dihydrate to prevent the environmental transmission of multidrug-resistant Acinetobacter Baumannii in hospital settings. Fresenius Environmental Bull 2020;29: 7191-7197. 
12. European Committee on Antimicrobial Susceptibility Testing. Available from: https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_11.0_Breakpoint_Tables.pdf Last, Accessed Date: 22.05.2021.
13. Rebelo AR, Bortolaia V, Kjeldgaard JS, Pedersen SK, Leekitcharoenphon P, Hansen IM, et al. Multiplex PCR for detection of plasmid-mediated colistin resistance determinants, mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 for surveillance purposes. Euro Surveill 2018; 23:17-00672.
14. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33:2233–2239.
15. Thatrimontrichai A, Pannaraj PS, Janjindamai W, Dissaneevate S, Maneenil G, Apisarnthanarak A. Intervention to reduce carbapenem-resistant Acinetobacter baumannii in a neonatal intensive care unit. Infect Control Hosp Epidemiol 2020; 41:710-5. 
16. Johnstone L, Spence S, Koziol-McClain J. Oral hygiene care in the pediatric intensive care unit: practise recommendations. Pediatr Nurs 2010;36: 85–97.
17. Nutman A, Temkin E, Lellouche J, Ben David D, Schwartz D, Carmeli Y. Detecting carbapenem-resistant Acinetobacter baumannii (CRAB) carriage: Which body site should be cultured? Infect Control Hosp Epidemiol 2020; 41:965-967. 
18. Da Collina GA, Tempestini-Horliana ACR, da Silva DFT, Longo PL, Makabe MLF, Pavani C. Oral hygiene in intensive care unit patients with photodynamic therapy: Study protocol for randomised controlled trial. Trials 2017;18:385-393. 
19. Silva E, Junior LD, Da Silveira FH, Moreno R, Vincent JL. Prevalence and outcomes of infections in Brazilian ICUs: a subanalysis of EPIC II study. Rev Bras Ter Intensiva 2012; 24:143–150.
20. Garcia R, Jendresky L, Colbert L, Bailey A, Zaman M, Majumder M. Reducing ventilator-associated pneumonia through advanced oral-dental care: a 48- month study. Am J Crit Care 2009; 18:523–534.
21. Azim A, Dwivedi M, Rao PB, Baronia AK, Singh RK, Prasad KN, et al. Epidemiology of bacterial colonization at intensive care unit admission with emphasis on extended-spectrum beta-lactamase- and metallo-beta-lactamase-producing Gram-negative bacteria--an Indian experience. J Med Microbiol 2010; 59:955-960. 
22. Chaari A, Mnif B, Bahloul M, Mahjoubi F, Chtara K, Turki O, et al. Acinetobacter baumannii ventilator-associated pneumonia: epidemiology, clinical characteristics, and prognosis factors. Int J Infect Dis 2013;17: e1225-1228.
23. Sunenshine RH, Wright MO, Maragakis LL, Harris AD, Song X, Hebden J, et al. Multidrug-resistant Acinetobacter infection mortality rate and length of hospitalization. Emerg Infect Dis 2007; 13:97–103.
24. Kwon KT, Oh WS, Song JH, Chang HH, Jung SI, Kim SW, et al. Impact of imipenem resistance on mortality in patients with Acinetobacter bacteraemia. J Antimicrob Chemother 2007; 59:525–530.
25. Central Asian and Eastern European Surveillance of Antimicrobial Resistance, Annual report 2018. World Health Organization, Denmark: 2019. 
26. Central Asian and Eastern European Surveillance of Antimicrobial Resistance, Annual report 2017. World Health Organization, Denmark: 2018. 
27. Umezawa K, Asai S, Ohshima T, Iwashita H, Ohashi M, Sasaki M, et al. Outbreak of drug-resistant Acinetobacter baumannii ST219 caused by oral care using tap water from contaminated hand hygiene sinks as a reservoir. Am J Infect Control 2015; 43:1249-1251. 

آمار
تعداد مشاهده مقاله: 907
تعداد دریافت فایل اصل مقاله: 633