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Chen, Xiao-Guang, Dou, Bing-Hua, An, Jin-Dou, Feng, Song, Liu, Na, Sheng, Guang-Yao. (1400). MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway. , 25(1), 46-52. doi: 10.22038/ijbms.2021.58963.13095
Xiao-Guang Chen; Bing-Hua Dou; Jin-Dou An; Song Feng; Na Liu; Guang-Yao Sheng. "MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway". , 25, 1, 1400, 46-52. doi: 10.22038/ijbms.2021.58963.13095
Chen, Xiao-Guang, Dou, Bing-Hua, An, Jin-Dou, Feng, Song, Liu, Na, Sheng, Guang-Yao. (1400). 'MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway', , 25(1), pp. 46-52. doi: 10.22038/ijbms.2021.58963.13095
Chen, Xiao-Guang, Dou, Bing-Hua, An, Jin-Dou, Feng, Song, Liu, Na, Sheng, Guang-Yao. MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway. , 1400; 25(1): 46-52. doi: 10.22038/ijbms.2021.58963.13095

MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway

Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 25، شماره 1، فروردین 2022، صفحه 46-52    اصل مقاله (1.05 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.58963.13095
نویسندگان
Xiao-Guang Chen؛ Bing-Hua Dou؛ Jin-Dou An؛ Song Feng؛ Na Liu؛ Guang-Yao Sheng*
Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, P.R. China
چکیده
Objective(s): MAGI2-AS3 is a cancer suppressor gene of multiple malignancies. Acute lymphoblastic leukemia (ALL) is an important type of leukemia that especially occurs in children. Our work evaluated the modulation of MAGI2-AS3 in ALL.
Materials and Methods: qPCR and Western blotting were adopted for detection of target molecular expression. Growth and apoptosis were determined by CCK8 assay and Annexin V/PI staining. Glycolysis was detected by commercial kits. The direct binding between miR-452-5p and MAGI2-AS3 or FOXN3 was assessed by luciferase reporter assay. Tumor growth was measured in nude mice in vivo.
Results: MAGI2-AS3 was down-regulated in ALL. Enforced expression of MAGI2-AS3 inhibited growth and glycolysis while promoting apoptosis of ALL cells. Moreover, MAGI2-AS3 up-regulated FOXN3 via sponging miR-452-5p. FOXN3 depletion abrogated MAGI2-AS3-mediated anti-cancer action. More importantly, MAGI2-AS3 repressed ALL cell growth in nude mice through regulation of miR-452-5p/FOXN3. 
Conclusion: MAGI2-AS3 inhibits ALL development via modulating miR-452-5p/FOXN3.
کلیدواژه‌ها
ALL؛ Apoptosis؛ FOXN3؛ Glycolysis؛ Growth؛ MAGI2-AS3؛ miR-452-5p
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