Vitexin ameliorates GCDC-induced hepatocyte injury through SIRT6 and JAK2/STAT3 pathways | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 13، دوره 24، شماره 12، اسفند 2021، صفحه 1717-1725 اصل مقاله (1.18 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2021.59424.13196 | ||
| نویسندگان | ||
| Chuang Zhang؛ Suolin Li* ؛ Chi Sun؛ Lin Liu؛ Yanbin Fang؛ Xiaofeng Yang؛ Xingxin Pan؛ Ben Zhang | ||
| Department of Pediatric Surgery, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China | ||
| چکیده | ||
| Objective(s): Vitexin, a natural flavonoid, is commonly found in many foods and traditional herbal medicines and has clear health benefits. However, the role of vitexin in cholestasis is presently unclear. This study investigated whether vitexin mitigated glycochenodeoxycholate (GCDC)-induced hepatocyte injury and further elucidated the underlying mechanisms. Materials and Methods: A cell counting kit-8 (CCK-8) assay was conducted to evaluate cell viability. The mitochondrial membrane potential (MMP, Δψm), reactive oxygen species (ROS) levels, and apoptosis rate of hepatocytes exposed to GCDC were detected by flow cytometry (FCM). We then measured the cytoprotective effects of vitexin against oxidative stress. The molecular signaling pathway was further investigated by using Western blotting and signaling pathway inhibitors. Results: Here, we showed that vitexin increased cell viability and reduced cell apoptosis, necroptosis, and oxidative stress in a dose-dependent manner in GCDC-treated hepatocytes. In addition, by using selective inhibitors, we further confirmed that inhibition of the JAK2/STAT3 pathway by vitexin was mediated by prolonged activation of Sirtuin 6 (SIRT6). Conclusion: Vitexin attenuated GCDC-induced hepatocyte injury via SIRT6 and the JAK2/STAT3 pathways. | ||
| کلیدواژهها | ||
| Apoptosis؛ Cholestasis؛ Glycochenodeoxycholic acid؛ Necroptosis؛ Oxidative stress؛ SIRT6؛ Vitexin | ||
| مراجع | ||
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