سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Widowati, Wahyu, Kusuma, Hanna, Arumwardana, Seila, Afifah, Ervi, Wahyuni, Cintani, Wijayanti, Cahyaning, Maulana, Muhamad, Rizal, Rizal. (1400). Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7). , 24(12), 1656-1665. doi: 10.22038/ijbms.2021.59348.13174
Wahyu Widowati; Hanna Sari Widya Kusuma; Seila Arumwardana; Ervi Afifah; Cintani Dewi Wahyuni; Cahyaning Riski Wijayanti; Muhamad Aldi Maulana; Rizal Rizal. "Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7)". , 24, 12, 1400, 1656-1665. doi: 10.22038/ijbms.2021.59348.13174
Widowati, Wahyu, Kusuma, Hanna, Arumwardana, Seila, Afifah, Ervi, Wahyuni, Cintani, Wijayanti, Cahyaning, Maulana, Muhamad, Rizal, Rizal. (1400). 'Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7)', , 24(12), pp. 1656-1665. doi: 10.22038/ijbms.2021.59348.13174
Widowati, Wahyu, Kusuma, Hanna, Arumwardana, Seila, Afifah, Ervi, Wahyuni, Cintani, Wijayanti, Cahyaning, Maulana, Muhamad, Rizal, Rizal. Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7). , 1400; 24(12): 1656-1665. doi: 10.22038/ijbms.2021.59348.13174

Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7)

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 24، شماره 12، اسفند 2021، صفحه 1656-1665    اصل مقاله (1.17 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.59348.13174
نویسندگان
Wahyu Widowati* 1؛ Hanna Sari Widya Kusuma2؛ Seila Arumwardana2؛ Ervi Afifah2؛ Cintani Dewi Wahyuni2؛ Cahyaning Riski Wijayanti2؛ Muhamad Aldi Maulana2؛ Rizal Rizal2، 3
1Faculty of Medicine, Maranatha Christian University, Jl. Surya Sumantri No. 65, Bandung 40164, West Java, Indonesia
2Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia
3Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16426, West Java, Indonesia 2 Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas
چکیده
Objective(s): Inflammation is thought to be the common pathophysiological basis for several disorders. Corilagin is one of the major active compounds which showed broad-spectrum biological and therapeutic activities, such as antitumor, hepatoprotective, anti-oxidant, and anti-inflammatory. This study aimed to evaluate the anti-oxidant and anti-inflammatory activities of corilagin in LPS-induced RAW264.7 cells.
Materials and Methods: Anti-oxidant activities were examined by free radical scavenging of H2O2, NO, and *OH. The safe concentrations of corilagin on RAW264.7 were determined by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay on RAW264.7 cell lines. The inflammation cells model was induced with LPS. The anti-inflammatory activities measured IL-6, TNF-α, NO, IL-1β, PGE-2, iNOS, and COX-2 levels using ELISA assay.
Results: The results showed that corilagin had a significant inhibition activity dose-dependently in scavenging activities toward H2O2, *OH, and NO with IC50 values 76.85 µg/ml, 26.68 µg/ml, and 66.64 µg/ml, respectively. The anti-inflammatory activity of corilagin also showed a significant decrease toward IL-6, TNF-α, NO, IL-1β, PGE-2, iNOS, and COX-2 levels at the highest concentration (75 µM) compared with others concentration (50 and 25 µM) with the highest inhibition activities being 48.09%, 42.37%, 65.69%, 26.47%, 46.88%, 56.22%, 59.99%, respectively (P<0.05).
Conclusion: Corilagin has potential as anti-oxidant and anti-inflammatory in LPS-induced RAW 264.7 cell lines by its ability to scavenge free radical NO, *OH, and H2O2 and also suppress the production of proinflammatory mediators including COX-2, IL-6, IL-1β, and TNF-α in RAW 264.7 murine macrophage cell lines.
کلیدواژه‌ها
Anti-inflammatory؛ Anti-oxidant؛ Corilagin؛ LPS؛ RAW 264.7
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