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Bayat, Samaneh, Rabbani Zabihi, Akram, Amel Farzad, Sara, Movafagh, Jebreel, Hashemi, Ezzat, Arabzadeh, Sepideh, Hashemi, Maryam. (1400). Evaluation of debridement effects of bromelain-loaded sodium alginate nanoparticles incorporated into chitosan hydrogel in animal models. , 24(10), 1404-1412. doi: 10.22038/ijbms.2021.58798.13060
Samaneh Bayat; Akram Rabbani Zabihi; Sara Amel Farzad; Jebreel Movafagh; Ezzat Hashemi; Sepideh Arabzadeh; Maryam Hashemi. "Evaluation of debridement effects of bromelain-loaded sodium alginate nanoparticles incorporated into chitosan hydrogel in animal models". , 24, 10, 1400, 1404-1412. doi: 10.22038/ijbms.2021.58798.13060
Bayat, Samaneh, Rabbani Zabihi, Akram, Amel Farzad, Sara, Movafagh, Jebreel, Hashemi, Ezzat, Arabzadeh, Sepideh, Hashemi, Maryam. (1400). 'Evaluation of debridement effects of bromelain-loaded sodium alginate nanoparticles incorporated into chitosan hydrogel in animal models', , 24(10), pp. 1404-1412. doi: 10.22038/ijbms.2021.58798.13060
Bayat, Samaneh, Rabbani Zabihi, Akram, Amel Farzad, Sara, Movafagh, Jebreel, Hashemi, Ezzat, Arabzadeh, Sepideh, Hashemi, Maryam. Evaluation of debridement effects of bromelain-loaded sodium alginate nanoparticles incorporated into chitosan hydrogel in animal models. , 1400; 24(10): 1404-1412. doi: 10.22038/ijbms.2021.58798.13060

Evaluation of debridement effects of bromelain-loaded sodium alginate nanoparticles incorporated into chitosan hydrogel in animal models

Iranian Journal of Basic Medical Sciences
دوره 24، شماره 10، دی 2021، صفحه 1404-1412    اصل مقاله (1.21 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.58798.13060
نویسندگان
Samaneh Bayat1؛ Akram Rabbani Zabihi1؛ Sara Amel Farzad2؛ Jebreel Movafagh3؛ Ezzat Hashemi4؛ Sepideh Arabzadeh5؛ Maryam Hashemi* 6، 7
1Scool of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
3Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Neurology and Neurological Science, Stanford University, Stanford, CA, USA
5Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
6Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
7Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Bromelain, a mixture of proteolytic enzymes from pineapple (Ananas comosus) is known as a potential debriding agent in burn treatment. In this research, the debridement efficiency of chitosan hydrogel loaded by sodium alginate-chitosan nanoparticles (NPs) containing bromelain (Br 10%-AG-CS NPs) was evaluated in animal models. 
Materials and Methods: The NPs were prepared using the ionic gelation technique and their properties were identified. Then, the debridement effect of bromelain NPs incorporated into chitosan hydrogel was evaluated 4 hr after wound treatment in animal models. 
Results: The particle size of positively charged Br-AG-Cs NPs was about 390±25 nm. The encapsulation efficiency of bromelain into AG-CS NPs was about 92%. The in vitro release profile showed that the maximum release of bromelain from NPs occurred during the first 4 hr (70%). The hydrogel structure did not significantly affect the profile release of bromelain in the formulation. After 6 months of storage at 4 and 25 °C, the synthesized NPs indicated no significant changes in bromelain activity. It was found that Br 10%-Ag-Cs NPs-CS hydrogel had the most beneficial effects on reducing necrotic tissues and resulted in re-epithelialization compared with other treated groups (negative and positive control, CS hydrogel, and Br 10%-CS hydrogel). 
Conclusion: Therefore, using this novel formulation can be considered a potential debridement agent.
کلیدواژه‌ها
Bromelain؛ Chitosan؛ Debridement؛ Nanoparticles؛ Sodium alginate
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