Azadpour, Mojgan, Farajollahi, Mohammad Morad, Dariushnejad, Hassan, Varzi, Ali Mohammad, Varezardi, Amir, Barati, Mitra. (1400). Effects of synthetic silymarin-PLGA nanoparticles on M2 polarization and inflammatory cytokines in LPS-treated murine peritoneal macrophages. , 24(10), 1446-1454. doi: 10.22038/ijbms.2021.59312.13161
Mojgan Azadpour; Mohammad Morad Farajollahi; Hassan Dariushnejad; Ali Mohammad Varzi; Amir Varezardi; Mitra Barati. "Effects of synthetic silymarin-PLGA nanoparticles on M2 polarization and inflammatory cytokines in LPS-treated murine peritoneal macrophages". , 24, 10, 1400, 1446-1454. doi: 10.22038/ijbms.2021.59312.13161
Azadpour, Mojgan, Farajollahi, Mohammad Morad, Dariushnejad, Hassan, Varzi, Ali Mohammad, Varezardi, Amir, Barati, Mitra. (1400). 'Effects of synthetic silymarin-PLGA nanoparticles on M2 polarization and inflammatory cytokines in LPS-treated murine peritoneal macrophages', , 24(10), pp. 1446-1454. doi: 10.22038/ijbms.2021.59312.13161
Azadpour, Mojgan, Farajollahi, Mohammad Morad, Dariushnejad, Hassan, Varzi, Ali Mohammad, Varezardi, Amir, Barati, Mitra. Effects of synthetic silymarin-PLGA nanoparticles on M2 polarization and inflammatory cytokines in LPS-treated murine peritoneal macrophages. , 1400; 24(10): 1446-1454. doi: 10.22038/ijbms.2021.59312.13161

Effects of synthetic silymarin-PLGA nanoparticles on M2 polarization and inflammatory cytokines in LPS-treated murine peritoneal macrophages

Iranian Journal of Basic Medical Sciences
دوره 24، شماره 10، دی 2021، صفحه 1446-1454    اصل مقاله (1.08 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.59312.13161
نویسندگان
Mojgan Azadpour1؛ Mohammad Morad Farajollahi2؛ Hassan Dariushnejad3، 4؛ Ali Mohammad Varzi4؛ Amir Varezardi4؛ Mitra Barati* 1
1Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
2Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences,Tehran, Iran
3Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
4Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
چکیده
Objective(s): Silymarin (SM) is a natural antioxidant compound with good anti-inflammatory effects, but its poor water solubility restricts its usage. Today, nanomaterial compounds (such as PLGA Poly D, L-lactic-co-glycolic acid) can provide a proper drug delivery system and help improve the accessibility of bioactive compounds to cells and tissues.
Materials and Methods: In this study, PLGA nanoparticles (NPs) containing SM (SM-PLGA) were synthesized and characterized and their biological effects were evaluated on M2 macrophage polarization to regulate inflammation. SM-PLGA NPs were fabricated by the oil in water emulsion (O/W) method. Macrophages (MQs) were isolated from mouse peritoneum by the cold RPMI lavage protocol. Primary mouse MQ cells were treated by SM and SM-PLGA NPs and then stimulated with lipopolysaccharide (LPS). M2 polarization was evaluated by measurements of cytokine secretion levels (TNF-α, IL1-β, and IL-10), flow cytometry markers (F4/80, CD11b, CD38, and CD206), and the expression of specific proteins (M2 Ym1 and Fizz1).
Results: SM-PLGA characterization showed that NPs were fabricated in the desired form. SM and SM-PLGA decreased pro-inflammatory cytokines (TNF-α and IL1-β) and increased IL10 as an anti-inflammatory cytokine. On the other hand, the M2-associated markers and proteins increased following treatment with SM and SM-PLGA. Post-hoc analysis indicated that these changes were more pronounced in the SM-PLGA group.
Conclusion: This study revealed that SM-PLGA could markedly promote M2 polarization, thereby providing a valuable medical approach against sepsis and septic shock.
کلیدواژه‌ها
Cytokines؛ Nanoparticles؛ PLGA compound؛ Peritoneal macrophages؛ Silymarin
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