Muddassir, Maria, Munir, Sadaf, Raza, Almas, Basirat, Ahmad, Ahmed, Muddassir, Farooq, Umar, Ahmed, Syed Shoaib, Naqvi, Syed Zeeshan Haider. (1400). Epidemiology and high incidence of Metallo-β-lactamase and AmpC-β-lactamases in nosocomial Pseudomonas aeruginosa. , 24(10), 1373-1379. doi: 10.22038/ijbms.2021.57293.12748
Maria Muddassir; Sadaf Munir; Almas Raza; Ahmad Basirat; Muddassir Ahmed; Umar Farooq; Syed Shoaib Ahmed; Syed Zeeshan Haider Naqvi. "Epidemiology and high incidence of Metallo-β-lactamase and AmpC-β-lactamases in nosocomial Pseudomonas aeruginosa". , 24, 10, 1400, 1373-1379. doi: 10.22038/ijbms.2021.57293.12748
Muddassir, Maria, Munir, Sadaf, Raza, Almas, Basirat, Ahmad, Ahmed, Muddassir, Farooq, Umar, Ahmed, Syed Shoaib, Naqvi, Syed Zeeshan Haider. (1400). 'Epidemiology and high incidence of Metallo-β-lactamase and AmpC-β-lactamases in nosocomial Pseudomonas aeruginosa', , 24(10), pp. 1373-1379. doi: 10.22038/ijbms.2021.57293.12748
Muddassir, Maria, Munir, Sadaf, Raza, Almas, Basirat, Ahmad, Ahmed, Muddassir, Farooq, Umar, Ahmed, Syed Shoaib, Naqvi, Syed Zeeshan Haider. Epidemiology and high incidence of Metallo-β-lactamase and AmpC-β-lactamases in nosocomial Pseudomonas aeruginosa. , 1400; 24(10): 1373-1379. doi: 10.22038/ijbms.2021.57293.12748

Epidemiology and high incidence of Metallo-β-lactamase and AmpC-β-lactamases in nosocomial Pseudomonas aeruginosa

Iranian Journal of Basic Medical Sciences
دوره 24، شماره 10، دی 2021، صفحه 1373-1379    اصل مقاله (706.47 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.57293.12748
نویسندگان
Maria Muddassir1؛ Sadaf Munir2؛ Almas Raza1؛ Ahmad Basirat3؛ Muddassir Ahmed4؛ Umar Farooq1؛ Syed Shoaib Ahmed5؛ Syed Zeeshan Haider Naqvi* 1
1Institute of Molecular Biology & Biotechnology (IMBB), The University of Lahore, Defence road campus, Lahore, Pakistan
2Department of Pathology, Combined Military Hospital, Lahore Cantt, Pakistan
3Department of Respiratory Medicine, Tallaght University Hospital, Dublin, Ireland
4M. Islam Medical College, Gujranwala, Pakistan
5Al-Aleem Centre for Advanced Studies and Research, Gulab Devi Educational Complex, Lahore, Pakistan
چکیده
Objective(s): Isolates producing Metallo-β-lactamase (MBL) have a significant impact on therapeutic and diagnostic layouts, plus their increased frequency has been reported globally. Determination of incidence of clinical isolates of Pseudomonas aeruginosa that are capable of producing MBL and AmpC-β-lactamases making them resistant to imipenem and cefoxitin.
Materials and Methods: Out of 1159 collected samples of urine, wound swabs, blood, tissue, and pus, the isolation rate of P. aeruginosa in the period of March 2020 to February 2021 was 22.0% (255/1159). Bacterial strains that were resistant towards imipenem were further processed for detecting the β-lactamase group of genes followed by statistical analysis of risk factors done based on clinical sample, gender, plus department of sample collection.
Results: The percentage of resistance against imipenem was found to be 53%. Out of 135 strains, phenotypic tests revealed MBLs incidence to be 61.5% by combination disc test and 81.5% by Modified Hodge test (MHT). Frequencies of blaIMP-1, blaVIM, blaSHV, blaTEM, and blaOXA genes were calculated to be 13%, 15%, 32%, 43%, and 21%, respectively. Co-expressions of blaMBLs (blaVIM and blaIMP-1) plus blaESBL (blaSHV, blaOXA, blaTEM) were detected using simplex and multiplex PCR. blaTEM, blaSHV, and blaOXA co-existed in 7.5% of clinical isolates. 5.5% of the isolates exhibited simultaneous expression of MBL/ESBL genes. 15% of the isolates resistant to cefoxitin were positive for the blaAmpC gene (17/114).
Conclusion: This is a pioneer report from Pakistan that concomitantly presents expression of blaVIM and blaIMP-1 with blaTEM, blaOXA, blaSHV, and blaAmpC in isolates of P. aeruginosa.
کلیدواژه‌ها
Antibiotic resistance؛ beta-Lactamases؛ Infections؛ MDR genes؛ Pseudomonas aeruginosa
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