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Guzel Tanoglu, Esra. (1400). Differential expressions of miR-223, miR-424, miR-145, miR-200c, miR-139 in experimental rat chronic pancreatitis model and their relationship between oxidative stress, endoplasmic reticulum stress, and apoptosis. , 24(9), 1301-1306. doi: 10.22038/ijbms.2021.57664.12823
Esra Guzel Tanoglu. "Differential expressions of miR-223, miR-424, miR-145, miR-200c, miR-139 in experimental rat chronic pancreatitis model and their relationship between oxidative stress, endoplasmic reticulum stress, and apoptosis". , 24, 9, 1400, 1301-1306. doi: 10.22038/ijbms.2021.57664.12823
Guzel Tanoglu, Esra. (1400). 'Differential expressions of miR-223, miR-424, miR-145, miR-200c, miR-139 in experimental rat chronic pancreatitis model and their relationship between oxidative stress, endoplasmic reticulum stress, and apoptosis', , 24(9), pp. 1301-1306. doi: 10.22038/ijbms.2021.57664.12823
Guzel Tanoglu, Esra. Differential expressions of miR-223, miR-424, miR-145, miR-200c, miR-139 in experimental rat chronic pancreatitis model and their relationship between oxidative stress, endoplasmic reticulum stress, and apoptosis. , 1400; 24(9): 1301-1306. doi: 10.22038/ijbms.2021.57664.12823

Differential expressions of miR-223, miR-424, miR-145, miR-200c, miR-139 in experimental rat chronic pancreatitis model and their relationship between oxidative stress, endoplasmic reticulum stress, and apoptosis

Iranian Journal of Basic Medical Sciences
مقاله 17، دوره 24، شماره 9، آذر 2021، صفحه 1301-1306    اصل مقاله (994.61 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.57664.12823
نویسنده
Esra Guzel Tanoglu* 1، 2
1University of Health Sciences Turkey, Institution of Medical Sciences, Department of Molecular Biology and Genetics, Istanbul, Turkey
2University of Health Sciences Turkey, Experimental Medicine Research and Application Center, Uskudar, 34662, Istanbul, Turkey
چکیده
Objective(s): This study aimed to research the roles of miR-139, miR-221, miR-200c, miR-145, miR-223, miR-424, and miR-377 in endoplasmic reticulum stress (ERS), oxidative stress (OS), fibrosis, and apoptosis processes in chronic pancreatitis (CP) rat model.
Materials and Methods: Fourteen rats were randomized into 2 groups (Group 1, sham group (n=7) and Group 2, CP group (n=7)). TGF-beta and malondialdehyde concentrations were measured in rat blood samples. qRT-PCR was used to investigate the expression levels of 7 miRNAs in the pancreas tissues. The correlations of mRNA undergoing significant changes with inflammation (TNF-α, IL-6), ERS (Ire1-α, Perk), apoptosis (Caspase 3, Bcl-2), OS (Cat, Gpx1), and fibrosis (α-Sma) were investigated.
Results: The biochemical results and histopathological scores in Group 1 were statistically significantly high compared with Group 2 (p <0.5). Expression levels of seven miRNAs (miR-200c, miR-145, miR-223, miR-424) were significantly higher, while miR-139 was significantly lower in CP. In our study, we found that miR-200c, miR-145, and miR-139 may contribute to CP progression and cellular processes based on the correlation between ERS, OS, apoptosis, and inflammation with miRNA expression levels.
Conclusion: miR-200c, miR-145, miR-139, miR-223, and miR-424 play roles in the CP model. They may be used as candidate biomarkers for the CP process.
کلیدواژه‌ها
Apoptosis؛ Chronic pancreatitis؛ MicroRNAs؛ Oxidative stress؛ Quantitative real-time polymerase chain reaction؛ Rat
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