Allogeneic tumor cell line-based vaccines: A good alternative to autologous and cancer stem cell vaccines in colorectal cancer | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 9، دوره 24، شماره 9، آذر 2021، صفحه 1231-1239 اصل مقاله (991.98 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2021.56732.12671 | ||
| نویسندگان | ||
| Fatemeh Rafieenia1؛ Elham Nikkhah2؛ Fatemeh Nourmohammadi3؛ Sousan Hosseini1؛ Abbas Abdollahi4؛ Nourieh Sharifi5؛ Mohsen Aliakbarian4؛ Mohammad Mahdi Forghani Fard3؛ Mehran Gholamin6؛ Mohammad Reza Abbaszadegan* 1، 7 | ||
| 1Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 2Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3Department of Biology, Damghan branch, Islamic Azad University, Damghan, Iran | ||
| 4Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 5Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 6Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 7Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| چکیده | ||
| Objective(s): Besides the uncertainty about colorectal cancer stem cell (CCSC) markers, isolating, purifying, and enriching CCSCs to produce CCSC vaccines is highly challenging. However, allogeneic vaccines developed from CRC cell lines can provide universal, comprehensive, inexpensive, simple, and fast approach to cancer treatment. Materials and Methods: CCSCs were isolated from human CRC tissue using the in vitro sphere formation assay and then characterized through gene expression analysis, in vivo and in vitro tumor formation assay, karyotyping, and surface marker detection. Subsequently, CCSCs and two CRC cell lines (HT-29 and SW-480) were inactivated with cisplatin (CDDP) and administrated as vaccines to the three groups of athymic C57BL/6 nude mice. Afterward, tumorigenesis was challenged with HT-29 cells. The antitumor effect of vaccines was evaluated by tumor and spleen examination and immune response analysis. The cytotoxic activity of splenocytes and serum levels of TGF-β and IFN-γ were measured by Calcein-AM cytotoxicity assay and enzyme-linked immunosorbent assay (ELISA), respectively. Results: The results of gene expression analysis showed that CCSCs are CD44+CD133-LGR5-. All vaccinations resulted in decreased tumor growth, spleen enlargement, enhanced serum level of IFN-γ and TGF-β, and increased cytotoxic activity of natural killer (NK) cells. The antitumor efficacy of the CCSC vaccine was not more than CRC cell line-based vaccines. Interestingly, the allogeneic SW-480 vaccine could effectively inhibit tumorigenesis. Conclusion: Despite the great challenge in developing CCSC vaccines, allogeneic vaccines based on CRC cell lines can efficiently induce antitumor immunity in CRC. | ||
| کلیدواژهها | ||
| Allogeneic؛ Autologous؛ Cancer stem cell؛ Colorectal cancer؛ Vaccine | ||
| مراجع | ||
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1. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 2018;68:394-424. 2. Nguyen M, Tipping Smith S, Lam M, Liow E, Davies A, Prenen H, et al. An update on the use of immunotherapy in patients with colorectal cancer. Expert Rev Gastroenterol Hepatol 2021;15:291-304. 3. Butterfield LH. Cancer vaccines. Bmj 2015;350:h988. 4. Chiang CL-L, Coukos G, Kandalaft LE. Whole tumor antigen vaccines: where are we? Vaccines 2015;3:344-372. 5. Khan ST, Montroy J, Forbes N, Bastin D, Kennedy MA, Diallo J-S, et al. Safety and efficacy of autologous tumour cell vaccines as a cancer therapeutic to treat solid tumours and haematological malignancies: a meta-analysis protocol for two systematic reviews. BMJ Open 2020;10: e034714. 6. Yarchoan M, Huang CY, Zhu Q, Ferguson AK, Durham JN, Anders RA, et al. A phase 2 study of GVAX colon vaccine with cyclophosphamide and pembrolizumab in patients with mismatch repair proficient advanced colorectal cancer. Cancer Med 2020;9:1485-1494. 7. Wu AA, Bever KM, Ho WJ, Fertig EJ, Niu N, Zheng L, et al. A phase II study of allogeneic GM-CSF–transfected pancreatic tumor vaccine (GVAX) with Ipilimumab as maintenance treatment for metastatic pancreatic cancer. Clin Cancer Res 2020;26:5129-5139. 8. Kim VM, Pan X, Soares KC, Azad NS, Ahuja N, Gamper CJ, et al. Neoantigen-based EpiGVAX vaccine initiates antitumor immunity in colorectal cancer. JCI Insight 2020;5:e136368. 9. Srivatsan S, Patel JM, Bozeman EN, Imasuen IE, He S, Daniels D, et al. Allogeneic tumor cell vaccines: the promise and limitations in clinical trials. Hum Vaccin Immunother 2014;10:52-63. 10. Zheng L, Edil BH, Soares KC, El-Shami K, Uram JN, Judkins C, et al. A safety and feasibility study of an allogeneic colon cancer cell vaccine administered with a granulocyte–macrophage colony stimulating factor–producing bystander cell line in patients with metastatic colorectal cancer. Ann Surg Oncol 2014;21:3931-3937. 11. Lei X, He Q, Li Z, Zou Q, Xu P, Yu H, et al. Cancer stem cells in colorectal cancer and the association with chemotherapy resistance. Med Oncol 2021;38:1-13. 12. O’Brien CA, Kreso A, Jamieson CH. Cancer stem cells and self-renewal. Clin Cancer Res 2010;16:3113-3120. 13. Li Y-f, Xiao B, Tu S-f, Wang Y-y, Zhang X-l. Cultivation and identification of colon cancer stem cell-derived spheres from the Colo205 cell line. Braz J Med Biol Res 2012;45:197-204. 14. Abbaszadegan MR, Bagheri V, Razavi MS, Momtazi AA, Sahebkar A, Gholamin M. Isolation, identification, and characterization of cancer stem cells: A review. J Cell Physiol 2017;232:2008-2018. 15. Ghazaey S, Mirzaei F, Ahadian M, Keifi F, Semiramis T, Abbaszadegan MR. Pattern of chromosomal aberrations in patients from north East iran. Cell J 2013;15:258-265. 16. Jacobsen AV, Yemaneab BT, Jass J, Scherbak N. Reference gene selection for qPCR is dependent on cell type rather than treatment in colonic and vaginal human epithelial cell lines. PloS one 2014;9:e115592. 17. Dowling CM, Walsh D, Coffey JC, Kiely PA. The importance of selecting the appropriate reference genes for quantitative real time PCR as illustrated using colon cancer cells and tissue. F1000Res 2016;5:99-114. 18. Wu D, Wang J, Cai Y, Ren M, Zhang Y, Shi F, et al. Effect of targeted ovarian cancer immunotherapy using ovarian cancer stem cell vaccine. J Ovarian Res 2015;8:1-10. 19. Monga SP, Wadleigh R, Sharma A, Adib H, Strader D, Singh G, et al. Intratumoral therapy of cisplatin/epinephrine injectable gel for palliation in patients with obstructive esophageal cancer. Am J Clin Oncol 2000;23:386-392. 20. Bagheri V, Abbaszadegan MR, Memar B, Motie MR, Asadi M, Mahmoudian RA, et al. Induction of T cell-mediated immune response by dendritic cells pulsed with mRNA of sphere-forming cells isolated from patients with gastric cancer. Life Sci 2019;219:136-143. 21. Sheikhi A, Jafarzadeh A, Kokhaei P, Hojjat-Farsangi M. Whole tumor cell vaccine adjuvants: comparing IL-12 to IL-2 and IL-15. Iran J Immunol 2016;13:148-166. 22. Neller MA, López JA, Schmidt CW, editors. Antigens for cancer immunotherapy. Semin Immunol 2008;20:286-295. 23. Network CGAR, Bell D. Berchuck a., Birrer M, Chien J, Cramer DW, et al. Integrated genomic analyses of ovarian carcinoma. Nature 2011;474:609-615. 24. Belderbos RA, Baas P, Berardi R, Cornelissen R, Fennell DA, van Meerbeeck JP, et al. A multicenter, randomized, phase II/III study of dendritic cells loaded with allogeneic tumor cell lysate (MesoPher) in subjects with mesothelioma as maintenance therapy after chemotherapy: dendritic cell Immunotherapy for mesothelioma (DENIM) trial. Transl Lung Cancer Res 2019;8:280-285. 25. Ghafar MTA, Morad MA, El-Zamarany EA, Ziada D, Soliman H, Abd-Elsalam S, et al. Autologous dendritic cells pulsed with lysate from an allogeneic hepatic cancer cell line as a treatment for patients with advanced hepatocellular carcinoma: a pilot study. Int Immunopharmacol 2020;82:106375. 26. van de Loosdrecht AA, van Wetering S, Santegoets SJ, Singh SK, Eeltink CM, den Hartog Y et al. A novel allogeneic off-the-shelf dendritic cell vaccine for post-remission treatment of elderly patients with acute myeloid leukemia. Cancer Immunol Immunother 2018;67:1505-1518. 27. Kajihara M, Takakura K, Ohkusa T, Koido S. The impact of dendritic cell–tumor fusion cells on cancer vaccines-past progress and future strategies. Immunotherapy 2015;7:1111-1122. 28. Rafiq S, Hackett CS, Brentjens RJ. Engineering strategies to overcome the current roadblocks in CAR T cell therapy. Nat rev Clin oncol 2020;17:147-167. 29. Chan JD, Lai J, Slaney CY, Kallies A, Beavis PA, Darcy PK. Cellular networks controlling T cell persistence in adoptive cell therapy. Nat Rev Immunol 2021;15:1-16. 30. Umut Ö, Gottschlich A, Endres S, Kobold S. CAR T cell therapy in solid tumors: a short review. Memo- Mag Eur Med Oncol 2021;14:1-7. 31. Schoenborn JR, Wilson CB. Regulation of interferon‐γ during innate and adaptive immune responses. Adv Immunol 2007;96:41-101. 32. Fan X-S, Wu H-Y, Yu H-P, Zhou Q, Zhang Y-F, Huang Q. Expression of Lgr5 in human colorectal carcinogenesis and its potential correlation with β-catenin. Int J Colorectal Dis 2010;25:583-590. 33. He S, Zhou H, Zhu X, Hu S, Fei M, Wan D, et al. Expression of Lgr5, a marker of intestinal stem cells, in colorectal cancer and its clinicopathological significance. Biomed Pharmacother 2014;68:507-513. 34. Kim YJ, Kang DH, Song GJ, Ahn TS, Son MW, Lee MS, et al. Clinical relevance of Lgr5 expression in colorectal cancer patients. Korean J Clin Oncol 2018;14:76-82. 35. Zhou X, Geng L, Wang D, Yi H, Talmon G, Wang J. R-Spondin1/LGR5 activates TGFβ signaling and suppresses colon cancer metastasis. Cancer Res 2017;77:6589-6602. 36. Horst D, Scheel SK, Liebmann S, Neumann J, Maatz S, Kirchner T, et al. The cancer stem cell marker CD133 has high prognostic impact but unknown functional relevance for the metastasis of human colon cancer. J Pathol 2009;219:427-434. 37. Szaryńska M, Olejniczak A, Kobiela J, Łaski D, Śledziński Z, Kmieć Z. Cancer stem cells as targets for DC-based immunotherapy of colorectal cancer. Sci Rep 2018;8:1-22. | ||
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