Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathway | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 11، دوره 24، شماره 9، آذر 2021، صفحه 1247-1253 اصل مقاله (1.19 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2021.56781.12678 | ||
| نویسندگان | ||
| Xi Jiang* 1، 2؛ Ying Hu2؛ Yingjie Zhou2؛ Jin Chen1؛ Chonglu Sun2؛ Ziwei Chen2؛ Changfeng Jing1؛ Lexing Xu2؛ Fuhe Liu2؛ Wenjuan Ni2؛ Xuefeng Yu2؛ Lei Chen2 | ||
| 1Zhejiang University Mingzhou Hospital, Ningbo, 315000, China | ||
| 2Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China | ||
| چکیده | ||
| Objective(s): This research was designed to determine the role of irisin in lipopolysaccharide (LPS)-induced endometritis in female mice. Materials and Methods: Animals were randomly assigned into sham, sham + irisin, LPS, LPS + irisin (0.1, 1, 10 μg/kg), and LPS + irisin + compound C groups. Histological features and expression of AMPK, NF-κB, inflammatory mediators, and oxidative stress markers were compared among different groups. Results: The results showed that LPS resulted in obvious uterus damage, meanwhile, the inflammatory mediators (COX-2, iNOS, IL-1β, IL-6, and TNF-α), as well as NF-κB in the uterine tissue, were significantly increased and the level of adenosine monophosphate-activated protein kinase (AMPK) was reduced. Nevertheless, pretreatment with irisin reversed the phenomena caused by LPS. Interestingly, compound C (AMPK inhibitor) abolished irisin’s effects on the uterus, which suggested that irisin’s beneficial function was achieved through regulating the AMPK-NF-κB pathway. Moreover, LPS-induced alterations of oxidative factors (MnSOD, GSH, and MDA) were reversed significantly by pretreatment with irisin. This data indicated irisin’s beneficial function was also related to antioxidation besides anti-inflammation. Conclusion: Our study implies that irisin is a potential therapeutic agent for endometritis. | ||
| کلیدواژهها | ||
| AMP-activated protein kinases؛ Endometritis؛ Inflammation؛ Irisin؛ Lipopolysaccharide NF-κB | ||
| مراجع | ||
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