Antioxidant, anti-inflammatory and protective potential of gallic acid against paraquat-induced liver toxicity in male rats | ||
| Avicenna Journal of Phytomedicine | ||
| دوره 11، شماره 6، بهمن و اسفند 2021، صفحه 633-644 اصل مقاله (800.51 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2021.18581 | ||
| نویسندگان | ||
| Ali Nouri1؛ Najmeh Salehi-Vanani2؛ Esfandiar Heidarian* 2 | ||
| 1Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 2Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran | ||
| چکیده | ||
| Objective: As a herbicide, paraquat is a toxic agent that has devastating effects on human health. Gallic acid, on the other hand, is a natural compound that its anti-oxidant values have been reported in previous studies. Given these, this study was designed to evaluate whether gallic acid could reduce the toxic effects of paraquat in the liver of rats. Materials and Methods: Six groups of rats were considered in this study. Group 1 (control group), group 2 (25 mg/kg of paraquat), group 3 (paraquat-plus-silymarin), and groups 4, 5, and 6 (paraquat together with gallic acid at the doses of 25, 50, and 100 mg/kg, respectively). After treatment, biochemical, oxidative, and histopathological parameters were evaluated in the rats. Results: We found that as compared to the control group, while paraquat reduced the hepatic levels of anti-oxidative compounds such as vitamin C (p<0.001), superoxide dismutase (SOD) (p<0.001), and catalase (CAT) (p<0.001), the toxic agent increased the serum levels of protein carbonyl (PC) (p<0.001), malondialdehyde (MDA) (p<0.05), and IL-1β (p<0.001). Paraquat also increased (p<0.05) both serum lipid profile and liver-associated markers in the rats. Nevertheless, gallic acid not only enhanced (p<0.05) the activity of vitamin C, SOD, and CAT but also remarkably reduced (p<0.05) the serum lipid profile, as well as the oxidative and inflammatory markers in the paraquat-treated rats. Gallic acid had also ameliorating effects on the damaged morphology of hepatocytes upon paraquat treatment. Conclusion: The results of this study suggested that gallic acid possesses reinforcing effects on the antioxidant defense system and could be administered to reduce the toxicity of paraquat. | ||
| کلیدواژهها | ||
| Paraquat؛ Gallic acid؛ Inflammation؛ Liver | ||
| مراجع | ||
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Abd El-Rahman N, Kamal El-Dein E, Abd ElHady A, Soliman SM. 2016. Effect of hesperidin on γ-radiation-and/or paraquat herbicide-induced biochemical, hematological and histopathological changes in rats. Pakistan J Zool, 48: 1407- 1415. Ahmad I, Shukla S, Kumar A, Singh BK, Kumar V, Chauhan AK, Singh D, Pandey HP, Singh C. 2013. Biochemical and molecular mechanisms of N-acetyl cysteine and silymarin-mediated protection against maneb-and paraquat-induced hepatotoxicity in rats. Chem Biol Interact, 201: 9-18. Akinloye O, Abioye O, Olaojoyetan O, Awosika O, Akinloye D. 2013. Dosedependent effects of paraquat on c-reactive protein, some lipid profile parameters and histology of tissues in male albino rats. Ife J Sci, 15: 189-195. Amin F, Memarzia A, Kazerani HR, Boskabady MH. 2020. Carvacrol and Zataria multiflora influenced the PPARγ agonist effects on systemic inflammation and oxidative stress induced by inhaled paraquat in rat. Iran J Basic Med Sci, 23: 930-939. Amin F, Memarzia A, Rad HK, Kazerani HR, Boskabady MH. 2021a. Carvacrol and PPARγ agonist, pioglitazone, affects inhaled paraquat-induced lung injury in rats. Sci Rep, 11: 1-15. Amin F, Roohbakhsh A, Memarzia A, Kazerani HR, Boskabady MH. 2021b. Immediate and late systemic and lung effects of inhaled paraquat in rats. J Hazard Mater, 415: 125- 136. Effects of gallic acid on paraquat-induced liver toxicity AJP, Vol. 11, No. 6, Nov-Dec 2021 643 Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72: 248-254. Carleton H, Drury R, Wallington E, 1980. Carleton's histological technique: Oxford University Press. USA. El-Rahman A, Kamal El-Dein E, AM AE-H, Soliman SM. 2016. Effect of hesperidin on γ-radiation-and/or paraquat herbicideinduced biochemical, hematological and histopathological changes in rats. Pakistan J Zool, 48: 1407-1415. Flohe L, 1984. Superoxide dismutase assays, Methods in enzymology. Elsevier, pp. 93- 104. Friedewald WT, Levy RI, Fredrickson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem, 18: 499-502. Gawarammana IB, Buckley NA. 2011. Medical management of paraquat ingestion. Br J Clin Pharmacol, 72: 745-757. Gaweł S, Wardas M, Niedworok E, Wardas P. 2004. Malondialdehyde (MDA) as a lipid peroxidation marker. Wiad Lek, 57: 453- 455. Hafez AM. 2009. Antigenotoxic activity of melatonin and selenium against genetic damage induced by paraquat. Aust J Basic & Appl Sci, 3: 2130-2143. Heidarian E, Nouri A. 2019. Hepatoprotective effects of silymarin against diclofenacinduced liver toxicity in male rats based on biochemical parameters and histological study. Arch Physiol Biochem, 29: 1-7. Heidarian E, Rafieian-Kopaei M, Khoshdel A, Bakhshesh M. 2014. Metabolic effects of berberine on liver phosphatidate phosphohydrolase in rats fed on high lipogenic diet: an additional mechanism for the hypolipidemic effects of berberine. Asian Pac J Trop Biomed, 4: 429-435. Heidarian E, Soofiniya Y. 2011. Hypolipidemic and hypoglycemic effects of aerial part of Cynara scolymus in streptozotocin-induced diabetic rats. J Med Plant Res, 5: 2717-2723. Jadon A, Bhadauria M, Shukla S. 2007. Protective effect of Terminalia belerica Roxb. and gallic acid against carbon tetrachloride induced damage in albino rats. J Ethnopharm, 109: 214-218. Karimi-Khouzani O, Heidarian E, Amini SA. 2017. Anti-inflammatory and ameliorative effects of gallic acid on fluoxetine-induced oxidative stress and liver damage in rats. Pharmacol Rep, 69: 830-835. Koekkoek W, van Zanten AR. 2016. Antioxidant vitamins and trace elements in critical illness. Nutr Clin Pract, 31: 457-474. Nouri A, Heidarian E, Amini-Khoei H, Abbaszadeh S, Basati G. 2019. Quercetin through mitigation of inflammatory response and oxidative stress exerts protective effects in rat model of diclofenacinduced liver toxicity. J Pharm Pharmacogn Res, 7: 200-212. Omaye ST, Turnbull JD, Sauberlich HE, 1979. Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids, Methods in enzymology. Elsevier, pp. 3-11. Omobowale TO, Oyagbemi AA, Ajufo UE, Adejumobi OA, Ola-Davies OE, Adedapo AA, Yakubu MA. 2018. Ameliorative effect of gallic acid in doxorubicin-induced hepatotoxicity in Wistar rats through antioxidant defense system. J Diet Suppl, 15: 183-196. Padma VV, Sowmya P, Felix TA, Baskaran R, Poornima P. 2011. Protective effect of gallic acid against lindane induced toxicity in experimental rats. Food Chem Toxicol, 49: 991-998. Priscilla DH, Prince PSM. 2009. Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats. Chem Biol Interact, 179: 118-124. Ramezannezhad P, Nouri A, Heidarian E. 2019. Silymarin mitigates diclofenac-induced liver toxicity through inhibition of inflammation and oxidative stress in male rats. J HerbMed Pharmacol, 8: 231-237. Reznick AZ, Packer L, 1994. Oxidative damage to proteins: spectrophotometric method for carbonyl assay, Methods in enzymology. Elsevier, pp. 357-363. Sharifi-Rigi A, Heidarian E, Amini SA. 2019. Protective and anti-inflammatory effects of hydroalcoholic leaf extract of Origanum vulgare on oxidative stress, TNF-α gene expression and liver histological changes in paraquat-induced hepatotoxicity in rats. Arch Physiol Biochem, 125: 56-63. Sultan M, Ben-Ari Z, Masoud R, Pappo O, Nouri et al. AJP, Vol. 11, No. 6, Nov-Dec 2021 644 Harats D, Kamari Y, Safran M. 2017. Interleukin-1α and Interleukin-1β play a central role in the pathogenesis of fulminant hepatic failure in mice. PLoS One, 12: e0184084. Wei T, Tian W, Liu F, Xie G. 2014. Protective effects of exogenous β-hydroxybutyrate on paraquat toxicity in rat kidney. Biochem Biophys Res Commun, 447: 666-671. Wellington K, Jarvis B. 2001. Silymarin: a review of its clinical properties in the management of hepatic disorders. BioDrugs, 15: 465-489. Wesseling C, De Joode BVW, Ruepert C, León C, Monge P, Hermosillo H, Partanen LJ. 2001. Paraquat in developing countries. Int J Occup Environ Health, 7: 275-286. Winek C. 1986. Drug and chemic | ||
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