Effect of turmeric extract on bone healing in an experimental model of femoral bone fracture | ||
Avicenna Journal of Phytomedicine | ||
دوره 12، شماره 3، مرداد و شهریور 2022، صفحه 197-212 اصل مقاله (1.08 M) | ||
نوع مقاله: Short communication | ||
شناسه دیجیتال (DOI): 10.22038/ajp.2021.18561 | ||
نویسندگان | ||
Shahab Ilka1؛ Afshin Heshmati1؛ Seyed Alireza Mirabdollahi* 1؛ Abdollah Jafarzadeh* 2؛ Farnaz Sedghy3؛ Fatemeh Bagheri4؛ Omid Azari5؛ Mohammad Ali Mohammadi6؛ Fatemeh Jafari Dareh Dar7؛ Moein Arabnadvi1 | ||
1Department of Orthopedics, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran | ||
2Department of Immunology, Medical School, Kerman University of Medical Sciences, Kerman, Iran. | ||
3Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran | ||
4Pathology and Stem Cell Research Center, Pathology Department, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran | ||
5Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran | ||
6Department of Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran | ||
7Department of Cardiology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran | ||
چکیده | ||
Objective: Following bone trauma, several factors participate in making a balance between the activity of osteoblasts and osteoclasts. The receptor activator of nuclear factor kappa B ligand (RANKL), receptor activator of nuclear factor kappa B (RANK), and osteoprotegerin (OPG) molecules play critical roles in the healing process via regulation of osteoclasts function. Turmeric is suggested to have an anti-osteogenic potential; however, its effect on accelerating bone healing has not been adequately studied. Here, we used a rat model of femur fracture to explore the effect of treatment with turmeric extract on the bone repair and the expression of RANK, RANKL, and OPG molecules. Materials and methods: Eight rats were subjected to surgery, randomly divided into two groups, and treated orally with turmeric (200 mg/kg), or olive oil. Four oil-treated rats without bone fracture were used as control group. After six weeks of treatment, the femurs of animals were examined for radiological, histological, and gene expression analysis. Results: X-ray radiography showed thicker callus and a more obscure fracture line in the turmeric group. Furthermore, higher osteoblast percentages but no osteoclasts were observed in turmeric-treated animals, representing better repair of bone in the fracture site. Also, real-time analyses showed that treatment with turmeric reduced RANK and RANKL expression (p <0.0001) and lowered RANKL/OPG ratio (p=0.01) in femoral bone tissue. Conclusion: Our findings indicated the turmeric ability to facilitate bone hemostasis and optimize the expression of key markers involved in the bone metabolism. | ||
کلیدواژهها | ||
Femur fracture؛ Bone healing؛ Turmeric extract؛ Curcumin؛ Experimental model | ||
مراجع | ||
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