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Elgohary, Rania, Abdelsalam, Rania, Abdel-Salam, Omar, khattab, Mahmoud, Salem, Neveen, El‐Khyat, Zakaria, Morsy, Fatma. (1400). Protective effect of cannabinoids on gastric mucosal lesions induced by water immersion restrain stress in rats. , 24(9), 1182-1189. doi: 10.22038/ijbms.2021.54338.12213
Rania Salama Elgohary; Rania M. Abdelsalam; Omar ME Abdel-Salam; Mahmoud M. khattab; Neveen A. Salem; Zakaria A. El‐Khyat; Fatma A. Morsy. "Protective effect of cannabinoids on gastric mucosal lesions induced by water immersion restrain stress in rats". , 24, 9, 1400, 1182-1189. doi: 10.22038/ijbms.2021.54338.12213
Elgohary, Rania, Abdelsalam, Rania, Abdel-Salam, Omar, khattab, Mahmoud, Salem, Neveen, El‐Khyat, Zakaria, Morsy, Fatma. (1400). 'Protective effect of cannabinoids on gastric mucosal lesions induced by water immersion restrain stress in rats', , 24(9), pp. 1182-1189. doi: 10.22038/ijbms.2021.54338.12213
Elgohary, Rania, Abdelsalam, Rania, Abdel-Salam, Omar, khattab, Mahmoud, Salem, Neveen, El‐Khyat, Zakaria, Morsy, Fatma. Protective effect of cannabinoids on gastric mucosal lesions induced by water immersion restrain stress in rats. , 1400; 24(9): 1182-1189. doi: 10.22038/ijbms.2021.54338.12213

Protective effect of cannabinoids on gastric mucosal lesions induced by water immersion restrain stress in rats

Iranian Journal of Basic Medical Sciences
مقاله 3، دوره 24، شماره 9، آذر 2021، صفحه 1182-1189    اصل مقاله (1.17 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.54338.12213
نویسندگان
Rania Salama Elgohary* 1؛ Rania M. Abdelsalam2؛ Omar ME Abdel-Salam3؛ Mahmoud M. khattab2؛ Neveen A. Salem1؛ Zakaria A. El‐Khyat3؛ Fatma A. Morsy4
1Narcotics, Ergogenics and Poisons Department, National Research Centre (NRC), Cairo, Egypt
2Pharmacology and Toxicology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
3Medical Biochemistry Department, National Research Centre (NRC), Cairo, Egypt
4Clinical Pathology Department, National Research Centre (NRC), Cairo, Egypt
چکیده
Objective(s): This study aimed to determine the impact of cannabinoid agonists and antagonists on the mucosal lesion progress in the stomach induced by water-immersion restraint stress (WIRS).   
Materials and Methods: Rats subjected to WIRS for 4 hr were treated with Dimethyl sulfoxide (DMSO), CBR1 agonist (NADA 1 mg/kg), CBR1 antagonist (Rimonabant 1 mg/kg), CBR2 agonist (GW405833 1 mg/kg) or CBR2 antagonist (AM630 1 mg/kg SC) 30 min before WIRS. Microscopic lesions, oxidative stress, inflammatory cytokines biomarkers, and (Myeloperoxidase) MPO in gastric tissues were determined.
Results: Results indicated development of severe gastric lesions with a substantial increase in the contents of (nitric oxide) NO, (malondialdehyde) MDA,  (interleukin-1 beta) IL-1β, MPO, (tumor necrosis factor-alpha) TNF-α, and a significant fall in the content of GSH and the activity of  PON-1 after WIRS.
Conclusion: Treatment with NADA and AM630 protected gastric tissues against ulcers as demonstrated by a decrease in the contents of MDA, TNF-α, MPO, and IL-1β along with an increase in the content of PON-1 activity and GSH in the stomach tissues. On the other hand, treatment with SR141716A or GW405833 showed no protective effects on ulcers development. It seems that cannabinoids exert their antioxidant potential and anti-inflammatory effects against  WIRS-induced gastric ulcers by activation of CB1R.
کلیدواژه‌ها
Anti-oxidant؛ Cannabinoid receptor؛ Stress؛ TNF-α؛ Ulcer
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