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Rasoulinejad, Seyed Ahmad, Akbari, Abolfazl, Nasiri, Khadijeh. (1400). Interaction of miR-146a-5p with oxidative stress and inflammation in complications of type 2 diabetes mellitus in male rats: antioxidant and anti-inflammatory protection strategies in type 2 diabetic retinopathy. , 24(8), 1078-1086. doi: 10.22038/ijbms.2021.56958.12706
Seyed Ahmad Rasoulinejad; Abolfazl Akbari; Khadijeh Nasiri. "Interaction of miR-146a-5p with oxidative stress and inflammation in complications of type 2 diabetes mellitus in male rats: antioxidant and anti-inflammatory protection strategies in type 2 diabetic retinopathy". , 24, 8, 1400, 1078-1086. doi: 10.22038/ijbms.2021.56958.12706
Rasoulinejad, Seyed Ahmad, Akbari, Abolfazl, Nasiri, Khadijeh. (1400). 'Interaction of miR-146a-5p with oxidative stress and inflammation in complications of type 2 diabetes mellitus in male rats: antioxidant and anti-inflammatory protection strategies in type 2 diabetic retinopathy', , 24(8), pp. 1078-1086. doi: 10.22038/ijbms.2021.56958.12706
Rasoulinejad, Seyed Ahmad, Akbari, Abolfazl, Nasiri, Khadijeh. Interaction of miR-146a-5p with oxidative stress and inflammation in complications of type 2 diabetes mellitus in male rats: antioxidant and anti-inflammatory protection strategies in type 2 diabetic retinopathy. , 1400; 24(8): 1078-1086. doi: 10.22038/ijbms.2021.56958.12706

Interaction of miR-146a-5p with oxidative stress and inflammation in complications of type 2 diabetes mellitus in male rats: antioxidant and anti-inflammatory protection strategies in type 2 diabetic retinopathy

Iranian Journal of Basic Medical Sciences
مقاله 9، دوره 24، شماره 8، آبان 2021، صفحه 1078-1086    اصل مقاله (724.91 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.56958.12706
نویسندگان
Seyed Ahmad Rasoulinejad1؛ Abolfazl Akbari* 2؛ Khadijeh Nasiri3
1Department of Ophthalmology, Ayatollah Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran
2Department of Physiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
3Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran
چکیده
Objective(s): This study aimed to evaluate the role of miR-146a-5p in the pathogenesis of diabetic retinopathy and its interaction with oxidative stress and inflammation in the ocular tissue of rats with type 2 diabetes mellitus (T2DM).
Materials and Methods: Twenty adult male Sprague Dawley rats (220 ±20 g) were randomly assigned to control and diabetic groups. A high-fat diet was used for three months to induce T2DM which was confirmed by the HOMA-IR index. After that, the levels of glucose and insulin in serum, HOMA-IR as an indicator of insulin resistance, the ocular level of oxidative markers, TNF‐α, IL-1β, MIPs, and MCP-1 along with ocular gene expression of NF-κB, Nrf2, and miR-146a-5p were evaluated.
Results: The level of lipid peroxidation along with metabolic and inflammatory factors significantly increased and the antioxidant enzyme activity significantly decreased in diabetic rats (p <0.05). The ocular expression of NF-κB and TNF-α increased and Nrf2, HO-1, and miR-146a-5p expression decreased in diabetic rats (p <0.05). In addition, a negative correlation between miR-146a-5p expression with NF-κB and HOMA-IR and a positive correlation between miR-146a-5p with Nrf2 were observed.
Conclusion: It can be concluded that miR-146a-5p may regulate Nrf2 and NF-κB expression and inflammation and oxidative stress in the ocular tissue of diabetic rats.
کلیدواژه‌ها
Diabetic retinopathy؛ Inflammation؛ MicroRNA؛ Oxidative stress؛ Type 2 diabetes
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