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Arya, Priyanka, Nabi, Sayima, Bhandari, Uma. (1400). Modulatory role of atorvastatin against high-fat diet and zymosan-induced activation of TLR2/NF-ƙB signaling pathway in C57BL/6 mice. , 24(8), 1023-1032. doi: 10.22038/ijbms.2021.55460.12409
Priyanka Arya; Sayima Nabi; Uma Bhandari. "Modulatory role of atorvastatin against high-fat diet and zymosan-induced activation of TLR2/NF-ƙB signaling pathway in C57BL/6 mice". , 24, 8, 1400, 1023-1032. doi: 10.22038/ijbms.2021.55460.12409
Arya, Priyanka, Nabi, Sayima, Bhandari, Uma. (1400). 'Modulatory role of atorvastatin against high-fat diet and zymosan-induced activation of TLR2/NF-ƙB signaling pathway in C57BL/6 mice', , 24(8), pp. 1023-1032. doi: 10.22038/ijbms.2021.55460.12409
Arya, Priyanka, Nabi, Sayima, Bhandari, Uma. Modulatory role of atorvastatin against high-fat diet and zymosan-induced activation of TLR2/NF-ƙB signaling pathway in C57BL/6 mice. , 1400; 24(8): 1023-1032. doi: 10.22038/ijbms.2021.55460.12409

Modulatory role of atorvastatin against high-fat diet and zymosan-induced activation of TLR2/NF-ƙB signaling pathway in C57BL/6 mice

Iranian Journal of Basic Medical Sciences
مقاله 3، دوره 24، شماره 8، آبان 2021، صفحه 1023-1032    اصل مقاله (1.01 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.55460.12409
نویسندگان
Priyanka Arya؛ Sayima Nabi؛ Uma Bhandari*
Department of Pharmacology, School of Pharmaceutical Education & Research (SPER), Jamia Hamdard (UGC approved deemed to be University, Govt. of India), New Delhi- 110062, India
چکیده
Objective(s): Accumulated evidence provides a strong connection between the immune system and vascular inflammation. The innate immune system’s main sensors are toll-like receptors (TLRs). Zymosan (Zym), a fungal product, induces an inflammatory response via activating TLR2 of the immune system. Atorvastatin, a potent statin, possesses pleiotropic effects including immunomodulatory, lipid-lowering, and anti-inflammatory. Therefore, the current study aimed to evaluate the protective role of atorvastatin against a high-fat diet (HFD) and Zym-induced vascular inflammation in C57BL/6 mice via modulation of TLR2/NF-ƙB signaling pathway.
Materials and Methods: In silico study was conducted to confirm the binding affinity of atorvastatin against TLR2. Under in vivo study, mice were divided into four groups: Normal control: normal standard chow-diet fed for 30 days + Zym vehicle (sterile PBS, 5 mg/ml on 8th day); HFD (30 days) + Zym (80 mg/kg, IP, on 8th day); HFD/Zym + atorvastatin vehicle (0.5% CMC, p.o., from 10th to 30th day); HFD/Zym + atorvastatin (3.6 mg/kg, p.o., from 10th to 30th day).
Results: Atorvastatin treatment along with HFD and Zym inhibited vascular inflammation by suppressing the levels of aortic TLR2, cardiac NF-ƙB and decrease in serum TNF-α and IL-6. Further, there was an increase in hepatic LDLR levels, resulting in a decrease in serum LDL-C and an increase in HDL-C levels. Histopathological examination of the aorta showed a reduction in plaque accumulation with the atorvastatin-treated group as compared with HFD and Zym-treated group.
Conclusion: Atorvastatin attenuates vascular inflammation mediated by HFD and Zym through suppression of TLR2, NF-ƙB, TNF-α, IL-6, and upregulation of LDLR levels.
کلیدواژه‌ها
Atorvastatin High؛ fat diet Inflammation Low؛ density lipoprotein؛ receptor Nuclear factor؛ kappa B Toll؛ like receptor Zymosan
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