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Sisakht, Mohsen, Khoshdel, Zahra, Mahmoodzadeh, Amir, Shafiee, Sayed Mohammad, Takhshid, Mohammad Ali. (1400). Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons. , 24(7), 978-985. doi: 10.22038/ijbms.2021.54796.12289
Mohsen Sisakht; Zahra Khoshdel; Amir Mahmoodzadeh; Sayed Mohammad Shafiee; Mohammad Ali Takhshid. "Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons". , 24, 7, 1400, 978-985. doi: 10.22038/ijbms.2021.54796.12289
Sisakht, Mohsen, Khoshdel, Zahra, Mahmoodzadeh, Amir, Shafiee, Sayed Mohammad, Takhshid, Mohammad Ali. (1400). 'Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons', , 24(7), pp. 978-985. doi: 10.22038/ijbms.2021.54796.12289
Sisakht, Mohsen, Khoshdel, Zahra, Mahmoodzadeh, Amir, Shafiee, Sayed Mohammad, Takhshid, Mohammad Ali. Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons. , 1400; 24(7): 978-985. doi: 10.22038/ijbms.2021.54796.12289

Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons

Iranian Journal of Basic Medical Sciences
مقاله 15، دوره 24، شماره 7، مهر 2021، صفحه 978-985    اصل مقاله (953.45 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.54796.12289
نویسندگان
Mohsen Sisakht1؛ Zahra Khoshdel1؛ Amir Mahmoodzadeh1؛ Sayed Mohammad Shafiee1؛ Mohammad Ali Takhshid* 2، 3
1Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of Medical Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
3Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Objective(s): Adrenomedullin (AM) has high expression in the spinal cord. In this study, we investigated the expression of AM and its receptor components, including calcitonin receptor-like receptor (CLR) and receptor activity modifying proteins (RAMPs) in dorsal root ganglion (DRG) and spinal motor (SM) neurons. Furthermore, the effects of AM on cAMP/cAMP response element-binding protein (CREB), AKT/glycogen synthase kinase-3 beta (GSK-3β) signaling pathways, and expressions of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) were evaluated.
Materials and Methods: Rat embryonic DRG and SM neurons were isolated, purified, and cultured. Real-time PCR was used to assess expressions of AM, CLR, and RAMPs. cAMP levels, p-CREB, BDNF, and NT-3 were determined using an enzyme-linked immunosorbent assay. p-AKT and p-GSK-3β levels were determined by western blotting. Real-time PCR showed expressions of AM, CLR, RAMP2, and RAMP3 in both DRG and SM neurons.
Results: AM increased cAMP accumulation and p-CREB levels in DRG and SM neurons. AM increased p-AKT and p-GSK-3β in DRG, but not SM neurons. AM significantly increased BDNF expression in both DRG and SM neurons. There was also an increase in NT-3 level in both DRG and SM neurons, which is statistically significant in SM neurons.
Conclusion: These results showed both DRG and SM neurons are targets of AM actions in the spinal cord. An increase in BDNF expression by AM in both DRG and SM neurons suggests the possible beneficial role of AM in protecting, survival, and regeneration of sensory and motor neurons.
کلیدواژه‌ها
Adrenomedullin؛ Cyclic AMP؛ Nerve growth factors؛ Signaling transduction؛ Spinal cord
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