سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

GULER, Mustafa, Tanyeli, Ayhan, GUZEL ERDOGAN, Derya, Eraslan, Ersen, Comakli, Selim, Polat, Elif, DOGANAY, Songul. (1400). Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation. , 24(7), 935-942. doi: 10.22038/ijbms.2021.57196.12736
Mustafa Can GULER; Ayhan Tanyeli; Derya GUZEL ERDOGAN; Ersen Eraslan; Selim Comakli; Elif Polat; Songul DOGANAY. "Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation". , 24, 7, 1400, 935-942. doi: 10.22038/ijbms.2021.57196.12736
GULER, Mustafa, Tanyeli, Ayhan, GUZEL ERDOGAN, Derya, Eraslan, Ersen, Comakli, Selim, Polat, Elif, DOGANAY, Songul. (1400). 'Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation', , 24(7), pp. 935-942. doi: 10.22038/ijbms.2021.57196.12736
GULER, Mustafa, Tanyeli, Ayhan, GUZEL ERDOGAN, Derya, Eraslan, Ersen, Comakli, Selim, Polat, Elif, DOGANAY, Songul. Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation. , 1400; 24(7): 935-942. doi: 10.22038/ijbms.2021.57196.12736

Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 24، شماره 7، مهر 2021، صفحه 935-942    اصل مقاله (875.66 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.57196.12736
نویسندگان
Mustafa Can GULER1؛ Ayhan Tanyeli* 1؛ Derya GUZEL ERDOGAN2؛ Ersen Eraslan3؛ Selim Comakli4؛ Elif Polat5؛ Songul DOGANAY2
1Department of Physiology, Atatürk University, Faculty of Medicine, Erzurum, Turkey
2Department of Physiology, Sakarya University, Faculty of Medicine, Sakarya, Turkey
3Department of Physiology, Yozgat Bozok University, Faculty of Medicine, Yozgat, Turkey
4Department of Pathology, Atatürk University, Veterinary Faculty, Erzurum, Turkey
5Department of Nutrition and Dietetics, Faculty of Health Sciences, Erzurum Technical University, Erzurum, Turkey
چکیده
Objective(s): This study aimed to determine anti-inflammatory, antioxidant, and antiapoptotic properties of urapidil (Ura) against ovarian torsion detorsion (T/D) injury in rats.
Materials and Methods: 40 female Wistar albino rats were grouped as sham, T/D, T/D+dimethyl sulfoxide (DMSO), T/D+Urapidil (Ura) 0.5 mg/kg (low dose), and T/D+Urapidil (Ura) 5 mg/kg (high dose) groups. In treatment groups, Ura was administered intraperitoneally just before detorsion. Biochemical parameters (TAS, TOS, MDA, MPO, and SOD) and immunohistochemical (IL-1β, TNF-α, NF-κB, LC3B, and Caspase-3) analyzes were performed.
Results: In the T/D group, OSI and MPO levels were elevated significantly while TAS values decreased compared with the sham group. A significant difference occurred in the low dose treatment group in TAS and OSI levels compared with the T/D group. In the high dose treatment group, significant elevation in TAS but  reduction in OSI and MDA levels were observed compared with the T/D group. Immunohistochemical staining resulted in IL-1β, TNF-α, NF-κB, LC3B, and caspase-3 immunopositivity in the T/D group, while Ura treatment decreased those parameters. Intensive congestion and hemorrhage were observed in the T/D group, but contrary to this, treatment groups had alleviated congestion and hemorrhage.
Conclusion: These results suggest that Ura demonstrated protective effects against ovarian T/D injury via anti-oxidative, anti-inflammatory, and anti-apoptotic features. 
کلیدواژه‌ها
Autophagy؛ Caspase-3؛ Detorsion؛ NF-κB؛ Ovarian؛ Torsion؛ Urapidil
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