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Fardsanei, Fatemeh, Soltan Dallal, Mohammad Mehdi, Zahraei Salehi, Taghi, douraghi, masoumeh, Memariani, Mojtaba, Memariani, Hamed. (1400). Antimicrobial resistance patterns, virulence gene profiles, and genetic diversity of Salmonella enterica serotype Enteritidis isolated from patients with gastroenteritis in various Iranian cities. , 24(7), 914-921. doi: 10.22038/ijbms.2021.54019.12142
Fatemeh Fardsanei; Mohammad Mehdi Soltan Dallal; Taghi Zahraei Salehi; masoumeh douraghi; Mojtaba Memariani; Hamed Memariani. "Antimicrobial resistance patterns, virulence gene profiles, and genetic diversity of Salmonella enterica serotype Enteritidis isolated from patients with gastroenteritis in various Iranian cities". , 24, 7, 1400, 914-921. doi: 10.22038/ijbms.2021.54019.12142
Fardsanei, Fatemeh, Soltan Dallal, Mohammad Mehdi, Zahraei Salehi, Taghi, douraghi, masoumeh, Memariani, Mojtaba, Memariani, Hamed. (1400). 'Antimicrobial resistance patterns, virulence gene profiles, and genetic diversity of Salmonella enterica serotype Enteritidis isolated from patients with gastroenteritis in various Iranian cities', , 24(7), pp. 914-921. doi: 10.22038/ijbms.2021.54019.12142
Fardsanei, Fatemeh, Soltan Dallal, Mohammad Mehdi, Zahraei Salehi, Taghi, douraghi, masoumeh, Memariani, Mojtaba, Memariani, Hamed. Antimicrobial resistance patterns, virulence gene profiles, and genetic diversity of Salmonella enterica serotype Enteritidis isolated from patients with gastroenteritis in various Iranian cities. , 1400; 24(7): 914-921. doi: 10.22038/ijbms.2021.54019.12142

Antimicrobial resistance patterns, virulence gene profiles, and genetic diversity of Salmonella enterica serotype Enteritidis isolated from patients with gastroenteritis in various Iranian cities

Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 24، شماره 7، مهر 2021، صفحه 914-921    اصل مقاله (1.14 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.54019.12142
نویسندگان
Fatemeh Fardsanei1؛ Mohammad Mehdi Soltan Dallal* 2، 1؛ Taghi Zahraei Salehi3؛ masoumeh douraghi1؛ Mojtaba Memariani4، 5؛ Hamed Memariani4، 6
1Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran
2Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
3Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Iran
4Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
6Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
چکیده
Objective(s): This study aimed to evaluate antibiotic resistance profiles and presence of virulence genes among Salmonella enterica serovar Enteritidis (S. Enteritidis) isolated from patients with gastroenteritis in various regions of Iran. Moreover, genetic relatedness among the strains was assessed by pulsed-field gel electrophoresis (PFGE).
Materials and Methods: From April through September 2017, 59 Salmonella strains were isolated from 2116 stool samples. Of these strains, 27 S. Enteritidis were recovered. These strains were subjected to disk diffusion tests, polymerase chain reaction (PCR) for detection of virulence genes (invA, hilA, pefA, rck, stn, ssrA, ssaR, sefA, spvC, sipA, sipC, sopB, sopE, and sopE2), and PFGE.
Results: High prevalence of resistance towards cefuroxime (n = 20, 74.1%) and ciprofloxacin (n = 13, 48.2%) were demonstrated. All tested strains possessed invA, hilA, sefA, sipA, sopB, and sopE. The least prevalent virulence gene was rck (n = 6; 22.2%). Based on combinations of virulence genes, 12 virulotypes were observed. The most common virulotype was VP2 (n = 12; 44.4%), harboring all of the virulence genes except for rck. PFGE typing showed only two distinct fingerprints among tested strains. Each fingerprint had completely different virulotypes. Notably, VP4 (harboring all genes except for rck and spvC) was only presented in pulsotype A, while VP2 was confined to pulsotype B.
Conclusion: S. Enteritidis strains were derived from a limited number of clones, suggesting that it is highly homogenous. Future works should consider combinations of other genotyping methods together with larger sample sizes from more diverse sources.
کلیدواژه‌ها
Antibiotic resistance؛ Gastroenteritis؛ PFGE؛ Salmonella enterica serotype Enteritidis؛ Virulence genes
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