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Alshinnawy, Ameera, El-Sayed, Wael, Sayed, Ahmed, Salem, Ahmed, Taha, AlShaimaa. (1400). Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved. , 24(6), 842-850. doi: 10.22038/ijbms.2021.56670.12655
Ameera Saeed Alshinnawy; Wael Mohamed El-Sayed; Ahmed AbdelAziz Sayed; Ahmed Mohamed Salem; AlShaimaa Mohamed Taha. "Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved". , 24, 6, 1400, 842-850. doi: 10.22038/ijbms.2021.56670.12655
Alshinnawy, Ameera, El-Sayed, Wael, Sayed, Ahmed, Salem, Ahmed, Taha, AlShaimaa. (1400). 'Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved', , 24(6), pp. 842-850. doi: 10.22038/ijbms.2021.56670.12655
Alshinnawy, Ameera, El-Sayed, Wael, Sayed, Ahmed, Salem, Ahmed, Taha, AlShaimaa. Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved. , 1400; 24(6): 842-850. doi: 10.22038/ijbms.2021.56670.12655

Telomerase activator-65 and pomegranate peel improved the health status of the liver in aged rats; multi-targets involved

Iranian Journal of Basic Medical Sciences
مقاله 16، دوره 24، شماره 6، شهریور 2021، صفحه 842-850    اصل مقاله (792.49 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.56670.12655
نویسندگان
Ameera Saeed Alshinnawy1؛ Wael Mohamed El-Sayed* 2؛ Ahmed AbdelAziz Sayed1، 3؛ Ahmed Mohamed Salem1؛ AlShaimaa Mohamed Taha1
1Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt. 11566
2Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt. 11566
3Children’s Cancer Hospital 57357, Cairo, Egypt
چکیده
Objective(s): This study was undertaken to investigate the efficacy of telomerase activator-65 (Ta-65) and pomegranate peel against aging-induced deteriorations in the liver.
Materials and Methods: The rats were divided into four groups: control, aged, aged rats treated with Ta-65, and pomegranate orally for two months.
Results: Aging significantly increased the serum levels of total protein, globulins, and protein carbonyl and reduced the insulin-like growth factor 1 (IGF-1). It also elevated the hepatic malondialdehyde and decreased the hepatic glutathione S-transferase (GST) activity. Aging elevated the expression of thioredoxin reductase1, telomerase reverse transcriptase, and cytochrome 3a1 in the liver; it increased the p53 protein level and elevated the activity of caspase-3 in the liver indicating the occurrence of apoptosis. The architecture of the liver deteriorated in the aged rats, as shown by both light and electron microscopy examinations. The liver of the aged rats had many apoptotic hepatocytes with shrunken nuclei. Many hepatocytes had dilated rough endoplasmic reticulum, many lysosomes, and many fat droplets. Administration of Ta-65 and pomegranate to the aged rats normalized most of the previous biochemical parameters and improved the liver architecture.
Conclusion: Ta-65 and pomegranate have anti-aging activity through targeting multiple cellular pathways. It is also noteworthy that Ta-65 was superior to pomegranate in its alleviative effects.
کلیدواژه‌ها
Aging؛ Apoptosis؛ IGF1؛ Oxidative stress؛ Telomerase؛ Thioredoxin reductase
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