Hidayat, Meilinah, Prahastuti, Sijani, Yusuf, Muhammad, Hassan, Khomaini. (1400). Nutrition profile and potency of RGD motif in protein hydrolysate of green peas as an antifibrosis in chronic kidney disease. , 24(6), 734-743. doi: 10.22038/ijbms.2021.50291.11459
Meilinah Hidayat; Sijani Prahastuti; Muhammad Yusuf; Khomaini Hassan. "Nutrition profile and potency of RGD motif in protein hydrolysate of green peas as an antifibrosis in chronic kidney disease". , 24, 6, 1400, 734-743. doi: 10.22038/ijbms.2021.50291.11459
Hidayat, Meilinah, Prahastuti, Sijani, Yusuf, Muhammad, Hassan, Khomaini. (1400). 'Nutrition profile and potency of RGD motif in protein hydrolysate of green peas as an antifibrosis in chronic kidney disease', , 24(6), pp. 734-743. doi: 10.22038/ijbms.2021.50291.11459
Hidayat, Meilinah, Prahastuti, Sijani, Yusuf, Muhammad, Hassan, Khomaini. Nutrition profile and potency of RGD motif in protein hydrolysate of green peas as an antifibrosis in chronic kidney disease. , 1400; 24(6): 734-743. doi: 10.22038/ijbms.2021.50291.11459

Nutrition profile and potency of RGD motif in protein hydrolysate of green peas as an antifibrosis in chronic kidney disease

Iranian Journal of Basic Medical Sciences
مقاله 4، دوره 24، شماره 6، شهریور 2021، صفحه 734-743    اصل مقاله (792.67 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.50291.11459
نویسندگان
Meilinah Hidayat* 1؛ Sijani Prahastuti1؛ Muhammad Yusuf2؛ Khomaini Hassan3
1Faculty of Medicine, Universitas Kristen Maranatha, Jalan Prof. Drg. Suria Sumantri 65 Bandung 40163, Indonesia
2Research Center for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, Jalan Singaperbangsa No. 2, Bandung 40133, Indonesia
3Faculty of Medicine, Universitas Jenderal Achmad Yani, Jalan Terusan Jenderal Sudirman, Cibeber, Kec. Cimahi Selatan, Cimahi 40531, Indonesia
چکیده
Objective(s): Fibrosis is the major cause of chronic kidney injury and the primary etiology in diabetic glomerulosclerosis. The initial study of protein hydrolysate of green peas hydrolyzed by bromelain (PHGPB) considered it to improve kidney function parameters and showed no fibrosis in histopathology features in gentamicin-induced nephrotoxicity rats. In the current study, we aimed to assess the nutrition profile and potency of RGD in PHGPB as antifibrosis in chronic kidney disease (CKD).
Materials and Methods: Green peas (Pisum sativum) were hydrolyzed by bromelain from pineapple juice to obtain PHGPB. The amino acid content of PHGPB was measured using the UPLC method, while the primary structure used LC-MS/MS. Bioinformatic analysis was conducted using the Protease Specificity Predictive Server (PROSPER). The potency of RGD in PHGPB was characterized by determining the levels of Fibronectin (FN) and TGF-β1 in mesangial SV40 MES 13 cell lines of diabetic glomerulosclerosis.
Results: The level of lysine was 364.85 mg/l. The LC-MS/MS data showed two proteins with 4–15 kDa molecular weight originated from convicilin (P13915 and P13919) which were predicted by PROSPER proteolytic cleavage, resulted in RGD in the LERGDT sequence peptide. PHGPB increased SV40 MES 13 mesangial cell proliferation that died from high-glucose levels (diabetic glomerulosclerosis model). PHGPB and RGD reduced the levels of FN and TGF-β1 in mesangial cell lines of diabetic glomerulosclerosis.
Conclusion: The nutrition profile and RGD motif in PHGPB show great potential as antifibrosis in CKD.
کلیدواژه‌ها
Convicilin protein؛ Fibronectin؛ Fibrosis؛ Peas؛ Pisum sativum؛ Protein hydrolysates؛ RGD motif؛ TGF-Beta 1
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