Rahmani, Behnaz, Astani, Akram, Zarei Jaliani, Hossein, Kheirandish, Mohammad Hassan, Mosadegh, Ahmad. (1400). Evaluation of kinetic stability and anti-staphylococcal activity of recombinant LasA protein produced in Escherichia coli. , 24(6), 851-855. doi: 10.22038/ijbms.2021.54563.12250
Behnaz Rahmani; Akram Astani; Hossein Zarei Jaliani; Mohammad Hassan Kheirandish; Ahmad Mosadegh. "Evaluation of kinetic stability and anti-staphylococcal activity of recombinant LasA protein produced in Escherichia coli". , 24, 6, 1400, 851-855. doi: 10.22038/ijbms.2021.54563.12250
Rahmani, Behnaz, Astani, Akram, Zarei Jaliani, Hossein, Kheirandish, Mohammad Hassan, Mosadegh, Ahmad. (1400). 'Evaluation of kinetic stability and anti-staphylococcal activity of recombinant LasA protein produced in Escherichia coli', , 24(6), pp. 851-855. doi: 10.22038/ijbms.2021.54563.12250
Rahmani, Behnaz, Astani, Akram, Zarei Jaliani, Hossein, Kheirandish, Mohammad Hassan, Mosadegh, Ahmad. Evaluation of kinetic stability and anti-staphylococcal activity of recombinant LasA protein produced in Escherichia coli. , 1400; 24(6): 851-855. doi: 10.22038/ijbms.2021.54563.12250

Evaluation of kinetic stability and anti-staphylococcal activity of recombinant LasA protein produced in Escherichia coli

Iranian Journal of Basic Medical Sciences
مقاله 17، دوره 24، شماره 6، شهریور 2021، صفحه 851-855    اصل مقاله (558.7 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.54563.12250
نویسندگان
Behnaz Rahmani1، 2؛ Akram Astani1، 3؛ Hossein Zarei Jaliani* 2؛ Mohammad Hassan Kheirandish2؛ Ahmad Mosadegh1
1Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2Department of Medical Biotechnology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3Zoonotic Diseases Research Center, Department of Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
چکیده
Objective(s): Staphylococcus aureus has become a major clinical concern due to the growing prevalence of multi-drug resistant (MDR) strains. Enzybioticts are peptidoglycan hydrolases that are recently introduced as an alternative agent to confront the MDR strains with a more effective mechanism than conventional antibiotics.  In this regard, our study aimed to evaluate the kinetic stability of LasA protease as a potent enzybiotic in the specific destruction of the S. aureus cell wall.
Materials and Methods: The catalytic domain of the Codon-optimized LasA gene was sub-cloned into pET28a vector, and BL21 DE3 cells were used for protein expression. Recombinant LasA protein was affinity purified by Ni-NTA column and staphylolytic activity of the LasA protein against methicillin-resistant strains was evaluated by disk diffusion and MIC test. The kinetic stability was evaluated in different temperatures during 48 hr.
Results: Our results revealed that LasA protein can completely prevent the growth of Methicillin-resistant S. aureus (MRSA) strain and inhibit the examined strain at the amount of 4 µg. furthermore, the catalytic domain of LasA protein can tolerate higher temperatures as well.
Conclusion: With regard to the failure of conventional antibiotics in treatment of MRSA infections, novel agents to combat multidrug-resistant strains are needed. The present study shows that LasA protein can eradicate MRSA strains, so it can be promising for the treatment of antibiotic-resistant staphylococci infection. The kinetic stability of LasA has also confirmed the possibility of industrial-scale manufacturing for the subsequent use of the enzyme clinically.
کلیدواژه‌ها
Antibiotic؛ LasA protease؛ MRSA؛ Stability؛ Staphylolysin
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