Verbascoside alleviates renal fibrosis in unilateral ureteral obstruction rats by inhibiting macrophage infiltration | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 6، دوره 24، شماره 6، شهریور 2021، صفحه 752-759 اصل مقاله (808.44 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2021.52759.11903 | ||
| نویسندگان | ||
| Guihua Zhang1؛ Fuxun Yu2؛ Rong Dong1، 3؛ Jiali Yu1، 3؛ Meng Luo1، 4؛ Yan Zha* 1، 3 | ||
| 1Guizhou University School of Medicine, Guizhou University, Gui Yang, Gui Zhou, China | ||
| 2The NHC Key Laboratory of Pulmonary Immunological Disease, Guizhou Provincial People’s Hospital, Gui Yang, Gui Zhou, China | ||
| 3Department of Nephrology, Guizhou Provincial People’s Hospital, Gui Yang, Gui Zhou, China | ||
| 4Department of Thoracic Surgery, Guizhou Provincial People’s Hospital, Gui Yang, Gui Zhou, China | ||
| چکیده | ||
| Objective(s): To explore the effect of verbascoside on renal fibrosis in unilateral ureteral obstruction (UUO) rats. Materials and Methods: Twenty Sprague-Dawley rats were randomly distributed into sham-operated, UUO, and UUO+Verbascoside groups. After two weeks of rat model construction, urine and blood samples were collected for biochemical analysis while kidney tissues were harvested for hematoxylin and eosin (H&E), Masson’s Trichrome, and immunohistochemistry staining. Pearson coefficient was used to analyze the correlation between the two proteins. Results: Verbascoside improved UUO-induced renal dysfunction as detected by decreased serum creatinine, urea nitrogen, and urinary protein excretion rate. In UUO rats, H&E staining result revealed increased total nucleated cell number, and Masson’s Trichrome staining results showed tubular interstitial fibrosis with the deposition of collagen fibrils. Besides, expressions of fibrosis-related proteins including collagen type I (COL-I), α-smooth muscle actin (a-SMA), and tissue inhibitor of metalloproteinase 2 (TIMP2) expressed higher in the UUO group. Moreover, macrophage infiltration-related factors such as CD68+, F4/80+ cells, and suppressor of cytokine signaling-3 (SOCS3) were significantly higher in the UUO group than in sham-operated rats. However, after administration with verbascoside, the accumulation of collagen fibrils and total nucleated cell numbers were mitigated. Likewise, macrophage infiltration was extenuated and fibrosis-related proteins were down-regulated in the UUO+Verbascoside rats. Correlation analysis indicated that macrophage infiltration-related markers were related to fibrosis-related factors. Conclusion: Verbascoside could alleviate renal fibrosis in UUO rats probably through ameliorating macrophage infiltration. | ||
| کلیدواژهها | ||
| Fibrosis؛ Macrophage infiltration؛ Obstructive nephropathy؛ Verbascoside؛ Renal fibrosis؛ Unilateral ureteral obstruction | ||
| مراجع | ||
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