سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Nazir, Humera, Aziz, Mubashar, Mirani, Zulfiqar, Sheikh, Ahsan, Saeed, Muhammad, Hussain, Masroor, Khan, Aleem, Ruby, Tahira, Rauf, Naseem. (1399). Correlation between antibiotic resistance and phylogenetic types among multidrug-resistant Escherichia coli isolated from urinary tract infections. , 24(3), 400-407. doi: 10.22038/ijbms.2021.47095.10865
Humera Nazir; Mubashar Aziz; Zulfiqar Ali Mirani; Ahsan Sattar Sheikh; Muhammad Qamar Saeed; Masroor Hussain; Aleem Ahmed Khan; Tahira Ruby; Naseem Rauf. "Correlation between antibiotic resistance and phylogenetic types among multidrug-resistant Escherichia coli isolated from urinary tract infections". , 24, 3, 1399, 400-407. doi: 10.22038/ijbms.2021.47095.10865
Nazir, Humera, Aziz, Mubashar, Mirani, Zulfiqar, Sheikh, Ahsan, Saeed, Muhammad, Hussain, Masroor, Khan, Aleem, Ruby, Tahira, Rauf, Naseem. (1399). 'Correlation between antibiotic resistance and phylogenetic types among multidrug-resistant Escherichia coli isolated from urinary tract infections', , 24(3), pp. 400-407. doi: 10.22038/ijbms.2021.47095.10865
Nazir, Humera, Aziz, Mubashar, Mirani, Zulfiqar, Sheikh, Ahsan, Saeed, Muhammad, Hussain, Masroor, Khan, Aleem, Ruby, Tahira, Rauf, Naseem. Correlation between antibiotic resistance and phylogenetic types among multidrug-resistant Escherichia coli isolated from urinary tract infections. , 1399; 24(3): 400-407. doi: 10.22038/ijbms.2021.47095.10865

Correlation between antibiotic resistance and phylogenetic types among multidrug-resistant Escherichia coli isolated from urinary tract infections

Iranian Journal of Basic Medical Sciences
مقاله 16، دوره 24، شماره 3، خرداد 2021، صفحه 400-407    اصل مقاله (556.38 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2021.47095.10865
نویسندگان
Humera Nazir1، 2؛ Mubashar Aziz* 2؛ Zulfiqar Ali Mirani3؛ Ahsan Sattar Sheikh4؛ Muhammad Qamar Saeed2؛ Masroor Hussain5؛ Aleem Ahmed Khan2؛ Tahira Ruby6؛ Naseem Rauf7
1Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan
2Institute of Pure and Applied Biology, (Microbiology division) Bahauddin Zakariya University, Multan 60900, Pakistan
3Department of Microbiology, PCSIR Laboratories Complex, Karachi 74000, Pakistan
4Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
5Department of Biotechnology, University of Science & Technology Bannu, Pakistan.
6Department of Zoology, The Islamia University of Bbahawalpur,61300, Bahawalpur, Pakistan
7PCSIR Laboratories Islamabad, Pakistan
چکیده
Objective(s): Emergence of multidrug resistance has reduced the choice of antimicrobial regimens for UTIs. To understand the association of phenotype and genotype among uropathogens.
Materials and Methods: Six hundred and twenty-eight (628) urine samples were collected and analyzed. Antibiotic sensitivity pattern was determined by the Kirby-Bauer Disc Diffusion Method and minimum inhibitory concentration (MIC) was tested by the E test. Fluoroquinolone resistant mutations in QRDR of gyrA and ParC, phylogenetic groups, and PAIusp subtype were detected by PCR.
Results: Most prevalent uropathogens were Escherichia coli (53.2%) followed by Klebsiella pneumoniae (21%). Multidrug- resistance was observed in > 50% cases for third-generation cephalosporins and ciprofloxacin and lowest in meropenem. E. coli (66.2%) and K. pneumonia (64.4%) were extended-spectrum β-lactamases (ESBLs) producers. MIC to trimethoprim-sulfamethoxazole was highest in E. coli (>1024 µg/ml). In 80 (24%) of the 334 E. coli isolates analyzed in detail, 54 fluoroquinolones (FQ) resistant isolates carried mutations (S83L, D87N, S80I, E84V) in QRDR of gyrA and ParC. Out of 54 FQ-resistant isolates, 43 (79.6%) isolates belonged to the phylogenetic group B2, and 11(20.4%) belonged to group D. Isolates belonged to group B2, 38 (88.4%) of the 43 isolates carried PAIusp subtype IIa and high frequency of mutation E84V in ParC was detected in 37 (97.4%). Other mutations, such as S80I, S83L in gyrA and D87N in ParC were found in all resistant isolates.
Conclusion: Correlations between phenotype and genotype provided a basis to understand the resistance development in uropathogens, and PAIusp subtyping indicated that E. coli belonged to the B2 group.
کلیدواژه‌ها
Escherichia coli؛ ESBL؛ MDR؛ PCR؛ UTI
سایر فایل های مرتبط با مقاله
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