Immunogenicity evaluation of the HIV-1 Tat containing polyepitope DNA vaccine adjuvanted with CpG-ODNs in mice | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 13، دوره 24، شماره 3، خرداد 2021، صفحه 377-382 اصل مقاله (678.06 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2021.52890.11923 | ||
| نویسندگان | ||
| Ehsan Ollah Jazaeri1؛ Atiyeh Mahdavi* 1؛ Asghar Abdoli2 | ||
| 1Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran | ||
| 2Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran | ||
| چکیده | ||
| Objective(s): HIV-1 is still considered a serious threat to human health, and accessibility of a suitable and efficient vaccine is urgently needed to address the disease burden. DNA vaccines employ the cells of the vaccinated hosts for in situ production of the vaccines. This strategy is an alternative and effective approach for traditional vaccination against high-risk pathogens, e.g., HIV-1. On the other hand, polyepitope vaccines, containing several immunogenic and conserved epitopes from virus vital regulatory and structural proteins, could more efficiently induce cellular and humoral immune responses against different clades of the virus. Materials and Methods: Herein, we comparatively investigated the immunogenic potency of the HIV-1 polytope DNA vaccine containing CpG oligodeoxynucleotides (CpG-ODNs) in BALB/c mice. To this end, after verifying the expression of the recombinant sequence in the eukaryotic HEK 293 cell line, it was amplified and extracted in the prokaryotic host cells (E. coli DH5α)) and then formulated and administered intramuscularly (IM) to the experimental mice (on days 0, 14, and 28) with and without CpG-ODNs adjuvant. Results: Taken together, the results demonstrated that CpG-ODNs adjuvanted DNA vaccine could significantly elicit cellular and humoral immune responses in the immunized animals in comparison with the control ones (p <0.05). Conclusion: Regarding the obtained results and also considering the advantages of polytopic and DNA vaccines, this approach might be considered a new regimen in HIV-1/AIDS vaccination. | ||
| کلیدواژهها | ||
| Cellular immunity CpG oligodeoxynucleotides DNA vaccine HIV؛ 1 Humoral immunity | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
1. Girard MP, Plotkin SA. HIV vaccine development at the turn of the 21st century. Curr Opin HIV AIDS 2012; 7:4-9. 2. Habibzadeh N, Bolhassani A, Vahabpour R, Sadat SM. How can improve DNA vaccine modalities as a therapeutic approach against HIV infections? J AIDS Clin Res 2015; 6:1-8. 3. Barouch DH. Challenges in the development of an HIV-1 vaccine. Nature 2008; 455:613-619. 4. Lavanya J, Saxena S, Jais M, Dutta R. DNA Vaccines-A Review. Jeevanu Times 2013; 13:12-16. 5. Liu M. DNA vaccines: A review. J Inter Med 2003; 253:402-410. 6. Tan L, Zhang Y, Liu F, Yuan Y, Zhan Y, Sun Y, et al. Infectious bronchitis virus poly-epitope-based vaccine protects chickens from acute infection. Vaccine 2016; 34:5209-5216. 7. Jazaeri EO, Mahdavi A, Abdoli A. Formulation of chitosan with the polyepitope HIV-1 protein candidate vaccine efficiently boosts cellular immune responses in mice. Pathog Dis 2017; 75:1-9. 8. Sun J, Hou J, Li D, Liu Y, Hu N, Hao Y, et al. Enhancement of HIV-1 DNA vaccine immunogenicity by BCG-PSN, a novel adjuvant. Vaccine 2013; 31:472-479. 9. Jafarpour N, Memarnejadian A, Aghasadeghi MR, Kohram F, Aghababa H, Khoramabadi N, et al. Clustered epitopes within a new poly-epitopic HIV-1 DNA vaccine shows immunogenicity in BALB/c mice. Mol Biol Rep 2014; 41:5207-5214. 10. Panahi Z, Abdoli A, Mosayebi G, Mahdavi M, Bahrami F. Subcutaneous administration CpG-ODNs acts as a potent adjuvant for an HIV-1-tat-based vaccine candidate to elicit cellular immunity in BALB/c mice. Biotechnol Lett 2018; 40:527-533. 11. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: Cold spring harbor laboratory press New York; 1989. 12. Kopycinski J, Cheeseman H, Ashraf A, Gill D, Hayes P, Hannaman D, et al. A DNA-based candidate HIV vaccine delivered via in vivo electroporation induces CD4 responses toward the α4β7-binding V2 loop of HIV gp120 in healthy volunteers. Clin Vaccine Immunol 2012; 19:1557-1559. 13. Cafaro A, Tripiciano A, Sgadari C, Bellino S, Picconi O, Longo O, et al. Development of a novel AIDS vaccine: The HIV-1 transactivator of transcription protein vaccine. Expert Opin Biol Ther 2015; 15:13-29. 14. Cicala C, Nawaz F, Jelicic K, Arthos J, S Fauci A. HIV-1 gp120: A target for therapeutics and vaccine design. Curr Drug Targets 2016; 17:122-135. 15. Yang Y, Zhu Q, Sun W, Guo J, Ning X, Li Q, et al. A recombinant multi-epitope protein MEP1 elicits efficient long-term immune responses against HIV-1 infection. Hum Vaccin Immunother 2017; 13:1-9. 16. Reguzova A, Antonets D, Karpenko L, Ilyichev A, Maksyutov R, Bazhan S. Design and evaluation of optimized artificial HIV-1 poly-T cell-epitope immunogens. PLoS one 2015; 10:e0116412. 17. Mahdavi M, Ebtekar M, Azadmanesh K, Mahboudi F, Khorramkhorshid H, Rahbarizadeh F, et al. HIV-1 Gag p24-Nef fusion peptide induces cellular and humoral immune response in a mouse model. Acta virologica 2010; 54:131-136. 18. Ahmed T, Borthwick NJ, Gilmour J, Hayes P, Dorrell L, Hanke T. Control of HIV-1 replication in vitro by vaccine-induced human CD8+ T cells through conserved subdominant Pol epitopes. Vaccine 2016; 34:1215-1224. 19. Alipour S, Mahdavi A. Boosting Tat DNA vaccine with Tat protein stimulates strong cellular and humoral immune responses in mice. Biotechnol Lett 2020; 42:505-517. 20.Paul S, Planque SA, Nishiyama Y, Hanson CV. A covalent HIV vaccine: is there hope for the future? Future Virol 2009;4:7-10. 21. Falahati Z, Mahdavi A, Hassani L. Physicochemical studies on the structural stability of the HIV-1 vaccine candidate recombinant Tat protein. Int J Biol Macromol 2020; 164:403-414. 22. Kadkhodayan S, Jafarzade BS, Sadat SM, Motevalli F, Agi E, Bolhassani A. Combination of cell penetrating peptides and heterologous DNA prime/protein boost strategy enhances immune responses against HIV-1 Nef antigen in BALB/c mouse model. Immunol Lett 2017; 188:38-45. 23. Arabi S, Aghasadeghi MR, Memarnejadian A, Kohram F, Aghababa H, Khoramabadi N, et al. Cloning, expression and purification of a novel multi-epitopic HIV-1 vaccine candidate: A preliminary study on immunoreactivity. 2014; ???: ???-???. 24. Agrawal S, Kandimalla ER. Medicinal chemistry and therapeutic potential of CpG DNA. Trends Mol Med 2002; 8:114-121. 25. Bode C, Zhao G, Steinhagen F, Kinjo T, Klinman DM. CpG DNA as a vaccine adjuvant. Expert Rev Vaccines 2011; 10:499-511. 26. Marciani DJ. Vaccine adjuvants: Role and mechanisms of action in vaccine immunogenicity. Drug discovery today 2003; 8:934-943. 27. Mutwiri G, Babiuk LA. Approaches to enhancing immune responses stimulated by CpG oligodeoxynucleotides. Adv Drug Deliv Rev 2009; 61:226-232. 28. Rees DC, Hartley MG, Green M, Lukaszewski RA, Griffin KF, Atkins HS, et al. The ability of CpG oligonucleotides to protect mice against Francisella tularensis live vaccine strain but not fully virulent F. tularensis subspecies holarctica is reflected in cell-based assays. Microb Pathog 2013; 63:16-18. 29.Caputo A, Gavioli R, Bellino S, Longo O, Tripiciano A, Francavilla V, et al. HIV-1 Tat-based vaccines: an overview and perspectives in the field of HIV/AIDS vaccine development. Int Rev Immunol 2009; 28:285-334. 30. Ballas ZK, Rasmussen WL, Krieg AM. Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA. J Immunol 1996; 157:1840-1845. 31. Alipour S, Mahdavi A, Abdoli A. The effects of CpG-ODNs and Chitosan adjuvants on the elicitation of immune responses induced by the HIV-1-Tat based candidate vaccines in mice. Pathog Dis 2017. 32.Kojima Y, Xin K-Q, Ooki T, Hamajima K, Oikawa T, Shinoda K, et al. Adjuvant effect of multi-CpG motifs on an HIV-1 DNA vaccine. Vaccine 2002; 20:2857-2865. | ||
|
آمار تعداد مشاهده مقاله: 603 تعداد دریافت فایل اصل مقاله: 300 |
||
