سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Tian, He, Zhao, Haosen, Mei, XIfan, Li, Daoyong, Lin, Jiaquan, Lin, Sen, Song, Changwei. (1399). Resveratrol inhibits LPS-induced apoptosis in VSC4.1 motoneurons through enhancing SIRT1-mediated autophagy. , 24(1), 38-43. doi: 10.22038/ijbms.2020.44534.10416
He Tian; Haosen Zhao; XIfan Mei; Daoyong Li; Jiaquan Lin; Sen Lin; Changwei Song. "Resveratrol inhibits LPS-induced apoptosis in VSC4.1 motoneurons through enhancing SIRT1-mediated autophagy". , 24, 1, 1399, 38-43. doi: 10.22038/ijbms.2020.44534.10416
Tian, He, Zhao, Haosen, Mei, XIfan, Li, Daoyong, Lin, Jiaquan, Lin, Sen, Song, Changwei. (1399). 'Resveratrol inhibits LPS-induced apoptosis in VSC4.1 motoneurons through enhancing SIRT1-mediated autophagy', , 24(1), pp. 38-43. doi: 10.22038/ijbms.2020.44534.10416
Tian, He, Zhao, Haosen, Mei, XIfan, Li, Daoyong, Lin, Jiaquan, Lin, Sen, Song, Changwei. Resveratrol inhibits LPS-induced apoptosis in VSC4.1 motoneurons through enhancing SIRT1-mediated autophagy. , 1399; 24(1): 38-43. doi: 10.22038/ijbms.2020.44534.10416

Resveratrol inhibits LPS-induced apoptosis in VSC4.1 motoneurons through enhancing SIRT1-mediated autophagy

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 24، شماره 1، فروردین 2021، صفحه 38-43    اصل مقاله (1015.28 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2020.44534.10416
نویسندگان
He Tian1؛ Haosen Zhao2؛ XIfan Mei* 2، 3؛ Daoyong Li2؛ Jiaquan Lin2؛ Sen Lin2؛ Changwei Song2
1Department of Histology and Embryology, School of Basic Medicine, Jinzhou Medical University, Liaoning 121000, China
2Department of Orthopedics, First Affiliated Hospital of Jinzhou Medical University, Liaoning 121000, China
3Medical College of Jinzhou Medical University, Liaoning 121000, China
چکیده
Objective(s): Resveratrol has been recognized as a potential therapeutic drug in spinal cord injury (SCI). Sirtuin 1 (SIRT1) is vital in the regulation of apoptosis and cell stress response. In this research, our purpose was to explore the mechanisms of resveratrol on neuroprotection and to explore the role of SIRT1.
Materials and Methods: We used lipopolysaccharide (LPS) in the VSC4.1 spinal cord neuron cell line to mimic the micro-environment of the injured spinal cord. The apoptosis of VSC4.1 motoneurons was assessed by TUNEL staining, Western blot, and RT-PCR. Immunofluorescence staining was used to observe the expression site of SIRT1, LC3-B, and Beclin-1, and their protein levels were measured by Western blot and RT-PCR.
Results: Our results showed that resveratrol inhibits LPS-induced apoptosis in VSC4.1 motoneurons. Levels of LC3-B, beclin-1, and SIRT1 indicated a significant increase after resveratrol treatment. But, if autophagy was inhibited, apoptosis in VSC4.1 motoneurons significantly increased. When the cells were treated with EX527, a SIRT1 inhibitor, the protein contents of LC3-B and Beclin-1 were suppressed.
Conclusion: Resveratrol inhibits apoptosis through promoting autophagy in VSC4.1 motoneurons. SIRT1 was involved in autophagy activated by resveratrol in VSC4.1 motoneurons.
کلیدواژه‌ها
Apoptosis؛ Autophagy؛ Lipopolysaccharide؛ Resveratrol؛ Sirtuin 1؛ Spinal cord injury
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